8,265 results
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Plasmid#111440PurposeN. castellii Solo CRISPR vector, marked with URA3 and NatRDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDA200
Plasmid#74248PurposepGPD1-dPSTRy(4,3)DepositorInsertpGPD1-dPSTRy(4,3)
ExpressionBacterial and YeastAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDBD-R
Plasmid#50571PurposeDouble Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572DepositorInsertDHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin
ExpressionYeastMutationM1R (B-gal)PromoterGal1Available SinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES
Plasmid#182485PurposeYeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked Free PcPTS
Plasmid#182486PurposeYeast integrative plasmid for expressing fusion protein ERG20-PcPTS, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertFPPS-PTS
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-ARF1-msGFP
Plasmid#105255PurposePlasmid for pop-in/pop-out replacement of Arf1 with Arf1-msGFP.DepositorAvailable SinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK59-ATG18-GFP
Plasmid#42529DepositorAvailable SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB2226
Plasmid#67533PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae X-4 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBJ1808 pRS416-COF1RFP
Plasmid#37103DepositorInsertCOF1-RFP
TagsRFPExpressionYeastMutationSingle RFP internal insertion at Cof147T48D with …Available SinceJune 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJW1665
Plasmid#112039PurposeIntegrating plasmid for expression of ER localized mCherry at TRP1 locusDepositorInsertER-mCherry
ExpressionYeastPromoterTPIAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TC-OM45-FKBPx2
Plasmid#104471PurposeIntegrating plasmid for overexpression of OM45-FKBPx2.DepositorAvailable SinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-RmMYB108
Plasmid#173183PurposeExpresses Rosa multiflora MYB108 for transcriptional activation studies in yeast.DepositorInsertRmMYB108
ExpressionYeastAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pROS13
Plasmid#107927Purposekan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pDIV489
Plasmid#177697PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRNR2p (Debaryomyces hansenii ) and TDH3p (Candida…Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
1000.pCCLsin.cPPT.hPGK.Myt1L.MIRT124_x4 Xma_lost.WPRE
Plasmid#67293PurposeInduce human iN conversion from fibroblastDepositorAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHES834
Plasmid#87947PurposeExpresses mKate2 under control of GAL1 promoter.DepositorInsertpGAL1-mKate2
UseSynthetic BiologyExpressionYeastPromoterpGal1Available SinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomApple-CaURA3
Plasmid#44879PurposeYeast tagging vector to create gene fusion with yeast optimized mApple with CaUra3 selectionDepositorInsertyomApple
ExpressionYeastAvailable SinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMJB1
Plasmid#160429PurposeYeast vector for expressing mCherry tagged genes under the GAL1 promoterDepositorTypeEmpty backboneTagsmCherryExpressionYeastPromoterGAL1Available SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS426-ACT_stable
Plasmid#127609Purposehigh-copy URA-selectable plasmid, Mut ACT1 intron (stable) inserted into URA3DepositorInsertStable ACT1 intron (Mut)
ExpressionYeastAvailable SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYC50
Plasmid#63906PurposeRecipient integrative vector to construct MYC tagged fusionsDepositorTypeEmpty backboneTagsMYCExpressionYeastAvailable SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAH6
Plasmid#41830PurposeUsed as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for ClonNAT resistanceDepositorTypeEmpty backboneUsePcr templateExpressionBacterial and YeastPromoterT7,TEFAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPt_Cp
Plasmid#206855PurposeA plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. The marker URA3 was integrated into the genome.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHES854
Plasmid#89197PurposeExpresses mKate2 under control of TDH3 constitutive promoter.DepositorInsertpTDH3-mKate2
ExpressionYeastPromoterTDH3Available SinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fSNAP-Kan
Plasmid#188918PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fSNAP-Kan
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Aga1p-FAPB2.3.6
Plasmid#158147PurposeYeast display reporter for non-display yeast strains (wild-type construct)DepositorInsertAga1p-FAPB2.3.6
ExpressionYeastPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOAD
Plasmid#196825PurposeYeast two-hybrid ; Gal4 activation domainDepositorTypeEmpty backboneTagsGal4 Activation domainExpressionYeastPromoterADHAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1TGt
Plasmid#44508DepositorInsertsUseSynthetic Biology; Expression regulator/reporterExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBT metaxin 2
Plasmid#12419DepositorInsertmetaxin 2 (Mtx2 Mouse)
ExpressionYeastAvailable SinceOct. 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
pXP742
Plasmid#46060DepositorTypeEmpty backboneExpressionYeastPromoterADH2Available SinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDS296
Plasmid#34985DepositorInsertePDZb1
ExpressionYeastPromoterGAL1Available SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
M4753 KanMX::ADE2 Disruptor Converter
Plasmid#51683PurposeYeast marker swap plasmid for converting KanMX3 to ADE2DepositorAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
NAT-TET-OFF -MET - 3xHA
Plasmid#182526PurposeDoxycycline-induced transcriptional regulation and 3xHA N-terminal tagging.DepositorInsert7 Copies of TetO Box
Tags3HAExpressionYeastAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pK3F-GAL1
Plasmid#85772PurposeN-ICE plasmid pK3F-GAL1DepositorInsertloxP-KanMX-loxP-GAL1p-3FLAG
UseCre/LoxExpressionYeastAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCP5
Plasmid#35397DepositorTypeEmpty backboneUseReporterExpressionYeastPromoterGDP promoterAvailable SinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pXP420 v2 (repaired Amp promoter)
Plasmid#86920PurposeShuttle vector to facilitate gene expression for metabolic engineering in S. cerevisiae. Contains TEF1 promoter and CYC1 terminator for insertion of gene of interest. Contains HIS3 selection marker.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastPromoterTEF1Available SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJT43_GalL_BE-P(AP)3
Plasmid#145036PurposeExpresses BE-P(AP)3 in yeast cellsDepositorInsertBE-(AP)3
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-mYFP
Plasmid#105144PurposeYeast gene targetingDepositorInsertkanMX6-P81nmt1-mYFP
Tags81nmt1-mYFPExpressionBacterial and YeastMutationA206KPromoter81nmt1Available SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
MtPT4
Plasmid#117096Purposeprotein expression in yeastDepositorInsertMtPT4
TagsHisExpressionYeastPromoterAOXAvailable SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT42_GalL_BE-PAPAPA
Plasmid#145035PurposeExpresses BE-PAPAPA in yeast cellsDepositorInsertBE-PAPAP
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fCLIP-URA
Plasmid#188922PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fCLIP-URA
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCT-L7.5.1
Plasmid#42900DepositorInsertL7.5.1
TagsHA and c-mycExpressionYeastPromoterGal1-10Available SinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGAN101
Plasmid#131123PurposePlasmid for overexpression of prion domains to induce prionsDepositorTypeEmpty backboneTagsmKate2ExpressionYeastAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pTH671-SUP35-H348Q
Plasmid#29732DepositorInsertSUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
ExpressionYeastMutationH348Q mutant gene of SUP35 including genomic sequ…Available SinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCfB2227
Plasmid#67536PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XI-3 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
LD101
Plasmid#69160PurposeIntegration of GFP tag at Atg1 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg1-EGFP
TagsEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMLB10
Plasmid#177273PurposeGAL1p:T7RNAP(F11L;T613A):CYC1tDepositorInsertT7RNAP(F11L;T613A)
ExpressionBacterial and YeastMutationF11L T613APromoterGAL1Available SinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomWasabi-SpHis5
Plasmid#44850PurposeYeast tagging vector to create gene fusion with yeast optimized mWasabi with SpHis5 selectionDepositorInsertyomWasabi
ExpressionYeastAvailable SinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPTK018-3b-RFP_Sec
Plasmid#84977PurposeType 3b, Red fluorescent proteinDepositorInsertRed fluorescent protein
UseSynthetic BiologyExpressionYeastMutationBsaI site removed (1677 c to g and 1794 a to g)PromoterNonAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only