8,350 results
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Plasmid#185886PurposeIn vivo gene amplification (HapAmp) of limonene synthetic module at RPL25 locus (14 copy).DepositorInsertsee comments
ExpressionYeastMutationERG20 F96W N127WAvailable SinceSept. 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNS1
Plasmid#131769PurposePADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sitesDepositorInsertFUSN_Fusion Red_Cry2WT
UseIntegration into δ-sitesExpressionYeastPromoterPADH1Available SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYC121
Plasmid#63917PurposeRecipient overexpressing vector to construct MYC tagged fusionsDepositorTypeEmpty backboneTagsMYCExpressionYeastPromoterTEF1 (Saccharomyces cerevisiae)Available SinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS315Nab3
Plasmid#110532PurposeExpresses yeast NAB3 using the endogenous promoterDepositorInsertNAB3 (NAB3 Budding Yeast)
ExpressionYeastAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
POM42-mTFP1
Plasmid#69852PurposeN-terminal tagging with mTFP1 using URA3 as a selection marker in S. cerevisiae.DepositorInsertmTFP1 minus start and stop codon
ExpressionYeastAvailable SinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG414GAL-ccdB-EGFP
Plasmid#14191DepositorTypeEmpty backboneUseGateway CloningTagsEGFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pIM572
Plasmid#74640Purposealias pFA6a-yTagRFP-T-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagRFP-T and G418 selection markerDepositorInsertyTagRFP-T
ExpressionYeastPromoternoneAvailable SinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV114
Plasmid#63181PurposepRS414 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pL
Plasmid#64177Purposeharbours marker gene LEU2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal TDP43 M337V
Plasmid#27460DepositorAvailable SinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoLSS-mKate2-CaURA3
Plasmid#44886PurposeYeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with CaUra3 selectionDepositorInsertyoLSS-mKate2
ExpressionYeastAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pV1329
Plasmid#111438PurposeSolo vector pV1326 +sgCgADE2DepositorInsertCaCas9/sgCgADE2
UseCRISPRExpressionYeastAvailable SinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pFA6a-hphMX6-P81nmt1-3FLAG
Plasmid#19350DepositorInserthphMX6
ExpressionYeastAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAG306GAL-ccdB-HA
Plasmid#14235DepositorTypeEmpty backboneUseGateway CloningTags3HAExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCYC1pr-GNSI (LEU)
Plasmid#111450PurposeGNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.DepositorInsertsCYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator
TagsmGFP5ExpressionYeastMutationM109I (Naturally occurring SNP) and The first 21 …Available SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZc-Ub-R-HsACTB*-thymosinB-8His
Plasmid#111149PurposeExpresses arginylated human beta-actin fused with thymosin beta and a His tag.DepositorInsertACTB (ACTB Human)
UsePichia pastoris integration plasmidTagsThymosin beta and a His-tagExpressionYeastMutationN-terminal arginylationPromoterAOX1Available SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pROS14
Plasmid#107928PurposeLEU2 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-nLuc
Plasmid#199756PurposeElongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stallingDepositorInsertsynYFP[TTG/AGA]-nLuc
ExpressionYeastPromoterTetO7Available SinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
LD211
Plasmid#69183PurposeIntegration of 2GFP tag at Atg11 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg11-2EGFP
Tags2xEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
M3499 ura3::ADE2 Disruptor Converter
Plasmid#51674PurposeYeast marker swap plasmid for converting URA3 to ADE2DepositorAvailable SinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICXC3H
Plasmid#49022PurposeFX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site and 10x His tagDepositorTypeEmpty backboneTags3C protease site (PreScission site) and decaHis (…ExpressionYeastPromoterAOX1Available SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-RPL13A-FKBPx2
Plasmid#104473PurposePlasmid for pop-in/pop-out replacement of RPL13A with an FKBPx2-tagged version.DepositorInsertRPL13Ap (RPL13A Budding Yeast)
TagsFKBPx2-HAExpressionYeastMutationincludes the last 354 bp of RPL13A onlyAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXP414
Plasmid#26841DepositorTypeEmpty backboneExpressionYeastAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP42
Plasmid#65518PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-Cit-attR1 and attR2-mCer-cMycExpressionYeastPromoterpPMA1Available SinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP35
Plasmid#65513PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-TFPt9-cMycExpressionYeastPromoterpPMA1Available SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP32
Plasmid#65511PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-t7CFPt9-cMycExpressionYeastPromoterpPMA1Available SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo
Plasmid#115420PurposeExpresses a regulatable secretory cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
ExpressionYeastPromoterTPI1Available SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSCupGFP
Plasmid#1086DepositorInsertGFP
ExpressionYeastAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFA CRU HISMX
Plasmid#131164PurposePCR template for genomic C-terminal Cub-R-URA3 tag, HISMX complements the histidine auxotrophy of S. cerevisiae his mutantDepositorTypeEmpty backboneTagsCRUExpressionYeastAvailable SinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA NuiHA-loxP-nat-CUP1p
Plasmid#131170PurposePCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to NourseothricinDepositorTypeEmpty backboneTagsNui-HAExpressionYeastAvailable SinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETCON-DnvLB16
Plasmid#45124DepositorInsertDnvLB16
TagsAga2p for yeast surface display and c-myc epitope…ExpressionYeastPromoterGALAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDH18
Plasmid#83803PurposeYFP-CFP positive FRET control; his selectionDepositorInsertYFP-CFP
ExpressionYeastAvailable SinceOct. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2190
Plasmid#67535PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSc-His6-Sem1Sc
Plasmid#83235PurposepGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeastDepositorAvailable SinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-ANP1-msGFP2
Plasmid#177100PurposeExpresses Anp1-msGFP2 in yeast cellsDepositorInsertANP1 (ANP1 Budding Yeast)
ExpressionYeastAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTY24
Plasmid#83794PurposeUseful for tagging yeast proteins with C-terminal DsRed tag; G418 selectionDepositorInsertDsRed
TagsDsRedExpressionYeastAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
1000.pCCLsin.cPPT.hPGK.Myt1L.MIRT124_x4 Xma_lost.WPRE
Plasmid#67293PurposeInduce human iN conversion from fibroblastDepositorAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG416GALL-ccdB-6stop
Plasmid#203511PurposeGateway-compatible destination vector for yeast expression with GALL promoter/6stop mutation/URA3 markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-3x-uC-ccdB-6stop
Plasmid#203514PurposeGateway-compatible destination vector for yeast expression with GAL1 promoter (3x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-ccdB-6stop
Plasmid#203512PurposeGateway-compatible destination vector for yeast expression with GAL10 promoter/6stop mutation/URA3 markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-1x-ccdB-6stop
Plasmid#203519PurposeGateway-compatible destination vector for yeast expression with GAL1 promoter (1x GAL4 binding sites)/6stop mutation/URA3 markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-2x-uA-ccdB-6stop
Plasmid#203517PurposeGateway-compatible destination vector for yeast expression with GAL1 promoter (2x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-DENV_NS3-GS-NS2B
Plasmid#201940PurposeExpresses DENV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 markerDepositorAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GALL-HIV-1_PR
Plasmid#203493PurposeExpresses HIV-1 protease from a GALL promoter with a URA3 markerDepositorInsertHIV-1 protease
ExpressionYeastPromoterGALLAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-WNV_NS2B-GS-NS3
Plasmid#203475PurposeExpresses WNV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 markerDepositorAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-SARS-CoV-2_PLP
Plasmid#203509PurposeExpresses SARS-CoV-2 papain-like protease from a GAL promoter with a URA3 markerDepositorAvailable SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-KUNV_NS2B-GS-NS3
Plasmid#203506PurposeExpresses KUNV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 markerDepositorInsertKUNV NS2B-GS-NS3
ExpressionYeastPromoterGAL1Available SinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-iGFP-TLG2
Plasmid#105262PurposePlasmid for pop-in/pop-out replacement of Tlg2 with iGFP-Tlg2.DepositorAvailable SinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pROS13
Plasmid#107927Purposekan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only