7,935 results

1. pJZC593

   Plasmid

   #62319

   Purpose

   sgRNA with MS2-PP7 for yeast cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   sgRNA + MS2-PP7 RNA binding module
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHUM

   Plasmid

   #64170

   Purpose

   harbours marker genes HIS3, URA3 and MET17

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. JME4393

   Plasmid

   #129657

   Purpose

   LYS5ex_CrisprCas9-yl_RFP: CAS9 vector with LYS5ex marker for gRNA cloning using GoldenGate

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNB813

   Plasmid

   #60161

   Purpose

   Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   yEVenus-PEST
   

   Use

   Tags

   PEST degron

   Expression

   Yeast

   Mutation

   Promoter

   SIC1pr

   Available since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLK89 - Edit-directing plasmid cloning insert

   Plasmid

   #98846

   Purpose

   Contains the insert used for step 2 of edit-directed plasmid cloning

   Depositor

   Leonid Kruglyak

   Insert

   KanR
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Changed sequence to remove a MluI cut site

   Promoter

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUB23-L-beta-gal

   Plasmid

   #8820

   Depositor

   Alexander Varshavsky

   Article

   Bachmair et al Science 1986 Oct 10;234(4773):179-86.

   Insert

   beta-gal
   

   Use

   Tags

   Ubiquitin

   Expression

   Yeast

   Mutation

   L (leucine) at amino terminus of beta-gal

   Promoter

   Available since

   Dec. 28, 2005

   Availability

   Academic Institutions and Nonprofits only

7. pAV0543

   Plasmid

   #133493

   Purpose

   Expression of nucleo-cytoplasmic mCherry in P-cells

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pLys3(BstZ17I)-p(map3)-mCherry-terminator(ScADH1)-natMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGBW-m4046332

   Plasmid

   #145413

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pAV0479

   Plasmid

   #133499

   Purpose

   Expression of sfGFP excluded from the nucleus with two NES signals

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pHis5(StuI)-p(tdh1)-NESwis1-sfGFP-NESmia1-natMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EZ-L767

    Plasmid

    #131773

    Purpose

    PADH1_sFUS_Fusion Red_PixD_TACT1

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    PADH1_sFUS_Fusion Red_PixD_TACT1
    

    Use

    Integration into δ-sites

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PADH1

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA6a-link-yoPS-CFP2-Kan

    Plasmid

    #44948

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with Kan selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoPS-CFP2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCfB4132

    Plasmid

    #106127

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFA6a-link-yoPS-CFP2-SpHis5

    Plasmid

    #44853

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoPS-CFP2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ZJOM108

    Plasmid

    #133665

    Purpose

    Integration of mTagBFP2-Tlg1 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-mTagBFP2-TLG1 (TLG1 Budding Yeast)
    

    Use

    Tags

    mTagBFP2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCLASP_Crz1(19A)_CLASP_RGS2membrane

    Plasmid

    #133085

    Purpose

    Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    Crz1*-CLASP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    pADH1

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGWKS12

    Plasmid

    #139419

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Superfolder GFP marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    Superfolder GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDR-GW FLIPglu-600μΔ13V

    Plasmid

    #28001

    Depositor

    Wolf Frommer

    Article

    Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

    Insert

    FLIPglu-600μΔ13V
    

    Use

    Tags

    eCFP and eYFP

    Expression

    Yeast

    Mutation

    F16A in mglB

    Promoter

    Available since

    March 31, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pVD_85

    Plasmid

    #149604

    Purpose

    (parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - XylR

    Depositor

    Michael Jensen

    Article

    Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

    Insert

    XylR
    

    Use

    Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pITI1

    Plasmid

    #166094

    Purpose

    Gal inducible expression of dCas9-FokI

    Depositor

    Rebecca Burgess

    Insert

    dCas9-FokI
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9-D10A, H840A

    Promoter

    Gal-inducible

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYS104

    Plasmid

    #1190

    Depositor

    Susan Lindquist

    Article

    Patino et al Science 1996 Aug 2;273(5275):622-6.

    Insert

    Hsp104 (HSP104 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wild-type Hsp104 sequence with 588bp of 5' p…

    Promoter

    Available since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

21. pUDP003

    Plasmid

    #101164

    Purpose

    E. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LHP462 - Ub-HA

    Plasmid

    #32169

    Depositor

    Linda Hicke

    Insert

    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

23. pVG48

    Plasmid

    #164706

    Purpose

    rtTA under the control of PGK1 promoter

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

    Insert

    PGK1pr - rtTA - CYC1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCfB2989

    Plasmid

    #63636

    Purpose

    pCfB2989 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJZC625

    Plasmid

    #62321

    Purpose

    sgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA +1x MS2, Pol II promoter with ribozyme cleavage
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pAdh

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDZ514 pURA MET25p PP7-PS-2x-yeGFP

    Plasmid

    #72230

    Purpose

    Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    PP7-PS-2x-yeGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pXP822

    Plasmid

    #46064

    Depositor

    Nancy DaSilva

    Article

    Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Osa-pCAMBIA-1301-STTM159

    Plasmid

    #84195

    Purpose

    Disturb the expression of miR159 in rice

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR159
    

    Use

    Tags

    Expression

    Bacterial, Plant, and Yeast…

    Mutation

    Promoter

    2x35S

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

29. pCfB3050(gRNA XII-5)

    Plasmid

    #73292

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. YCPlac33-sGFP-VRG4

    Plasmid

    #25447

    Depositor

    Benjamin Glick

    Article

    Losev et al Nature. 2006 Jun 22. 441(7096):1002-6.

    Insert

    Synthetic Green Fluorescent Protein - VRG4
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGBT9-Hook2

    Plasmid

    #198524

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    Hook2 (HOOK2 Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    His488Gln substitution

    Promoter

    ADH1

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJW1673

    Plasmid

    #112047

    Purpose

    CEN/ARS plasmid for GAL-inducible SCT1-V5-TEV-EGFP expression marked with URA

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    SCT1
    

    Use

    Tags

    V5-TEV-EGFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRS415-SOH1

    Plasmid

    #8622

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics 2004 Mar;166(3):1215-27.

    Insert

    SOH1 (SOH1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Wild type

    Promoter

    Available since

    Nov. 15, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCri-15a

    Plasmid

    #61323

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSK247

    Plasmid

    #131148

    Purpose

    dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mNeonGreen

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pSUP35, min pCYC1

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFA6a-hphMX6-P41nmt1-3FLAG

    Plasmid

    #19349

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Aug 26. ():.

    Insert

    hphMX6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pI3-MTU-DO-HPH

    Plasmid

    #160207

    Purpose

    Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Hygromycin selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pI3-MTU-DO-LEU

    Plasmid

    #160176

    Purpose

    Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Leucine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pI4-MTU-DO-LEU

    Plasmid

    #160029

    Purpose

    Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Leucine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pI3-MTU-DO-HIS

    Plasmid

    #160021

    Purpose

    Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Histidine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pI3-MTU-DO-URA

    Plasmid

    #160020

    Purpose

    Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Uracil selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pMTU-DO-LEU

    Plasmid

    #160006

    Purpose

    Low-copy expression vector for K. marxianus, leucine selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pZ_P-GAP-ACS1

    Plasmid

    #126712

    Purpose

    yeast plasmid complementing ACS1 from Komagataella phaffii

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    acs1
    

    Use

    Tags

    6xHis,Myc

    Expression

    Yeast

    Mutation

    Promoter

    GAP

    Available since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pFA6a-link-mKO1-SpHis5

    Plasmid

    #44642

    Purpose

    Yeast tagging vector to create gene fusion with mKO1 with SpHis5 selection

    Depositor

    Kurt Thorn

    Insert

    mKO1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pFA6a-bleMX6-Purg1-3FLAG

    Plasmid

    #19357

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Aug 26. ():.

    Insert

    urg1 promoter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. YIplac211-iGFP-YPT31

    Plasmid

    #105263

    Purpose

    Plasmid for pop-in/pop-out replacement of Ypt31 with iGFP-Ypt31.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Ypt31 (YPT31 )
    

    Use

    Tags

    iGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pJT112_GalL_nCDA1-xBE3

    Plasmid

    #141318

    Purpose

    Expressing base editor nCDA1-xBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    nCDA1-xBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

    Promoter

    GalL

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pRPR1_c3gRNA_RPR1t

    Plasmid

    #64381

    Purpose

    encodes c3 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    c3 gRNA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

49. HsCERK

    Plasmid

    #118600

    Purpose

    Expresses ceramide kinase, which catalyzes the phosphorylation of ceramide to form ceramide 1-phosphate

    Depositor

    Kevin Lynch

    Article

    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

    Insert

    Homo sapiens ceramide kinase
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    GAL1, 10

    Available since

    Feb. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. RPL25NLS-GFP (101)

    Plasmid

    #24038

    Depositor

    Michael Rout

    Insert

    RPL25 (RPL25 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Only bp 1-135 (aa 1-45) of RPL25

    Promoter

    Available since

    March 10, 2010

    Availability

    Academic Institutions and Nonprofits only