8,173 results
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Plasmid#27277DepositorAvailable SinceFeb. 1, 2011AvailabilityAcademic Institutions and Nonprofits only
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pRS403-PGAL1-weakSC_GFP
Plasmid#22315DepositorInsertGFP-rad9
UseIntegrating plasmidExpressionYeastAvailable SinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pXRhMX
Plasmid#63146PurposeHPH marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxExpressionBacterial and YeastAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAV0756
Plasmid#133508PurposeExpression of 3mCherry tagged CRIB domain to monitor active Cdc42DepositorInsertpHis5(StuI)-p(act1)-CRIB(gic2aa2-181)-3mCherry-bsdMX
ExpressionYeastAvailable SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pH3F
Plasmid#85773PurposeN-ICE plasmid pH3FDepositorInsertloxP-HphMX-loxP-3FLAG
UseCre/LoxExpressionYeastAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ube2d2
Plasmid#31410DepositorAvailable SinceOct. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-3nmt1-mMaple3
Plasmid#105167PurposeYeast gene targetingDepositorInsertkanMX6-3nmt1-mMaple3
Tags3nmt1-mMaple3ExpressionBacterial and YeastPromoter3nmt1Available SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-3xHA-LINKER
Plasmid#52686PurposeSame as 41024, with additional linkerDepositorInserttetO7-Pcyc1-3xHA+LINKER
UsePromoter replacementExpressionYeastAvailable SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-APL5-iGFPx6
Plasmid#105252PurposePlasmid for pop-in/pop-out replacement of Apl5 with Apl5-iGFPx6.DepositorAvailable SinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRP1454
Plasmid#64510PurposeExpresses destabilized Citrine in yeast.DepositorInsertCitrine-ADH1-tail
ExpressionYeastPromoterTDH3Available SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
FRP1671
Plasmid#64538PurposeInducible MET15 geneDepositorAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
nuc-yHS1-M7A,H102A
Plasmid#159168PurposeNuclear labile heme reporterDepositorInsertnuc-yHS1-M7A,H102A
TagsSV40 NLSExpressionYeastMutationMutated both Met 7 and His 102 of the cyt b562 moā¦PromoterGPDAvailable SinceSept. 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
mito-yHS1-M7A,H102A
Plasmid#159165PurposeMitochondrial labile heme reporterDepositorInsertmito-yHS1-M7A,H102A
TagsCOX4 pre-sequenceExpressionYeastMutationMutated both Met 7 and His 102 of the cyt b562 moā¦PromoterGPDAvailable SinceSept. 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
HisSumoOphP
Plasmid#189672PurposeHisSUMO tagged OphP to express OphP in pichia pastorias.DepositorInsertHisSumoOphP
TagsHisSumo taggedExpressionYeastAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
GPD 104QDProGFP p416
Plasmid#15569DepositorInserthtt 104Q Delta Pro (HTT Human)
TagsGFPExpressionYeastMutationProline-rich region removedAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNB777
Plasmid#60153PurposeRed click beetle luciferase (Promega) driven by LEU1 promoter.DepositorInsertCBR
ExpressionYeastPromoterLEU1prAvailable SinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-vps10-104
Plasmid#115426PurposeGenerate the vps10-104 allele via pop-in pop-out mutagenesisDepositorInsertvps10-104
ExpressionYeastMutationdomain 1 deletionPromoterEndogenousAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNC951
Plasmid#20400DepositorInsertCFP-reporter
ExpressionYeastMutationX=UbiY (see schematic) linker=deltakAvailable SinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pXRLy2
Plasmid#63149PurposeLys2 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxExpressionBacterial and YeastAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG414GAL-EGFP-ccdB
Plasmid#14311DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
LHP556 - UBC4
Plasmid#32440DepositorAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pZEN
Plasmid#129113PurposeZ. rouxii episomal vector containing ClonNAT resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZCAG
Plasmid#129114PurposeZ. rouxii centromeric vector containing G418 resistanceDepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-ccdB-EGFP
Plasmid#14192DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAV0764
Plasmid#133520PurposeExpression of mCherry targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)DepositorInsertpLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-mCherry-AHDL-bsdMX
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAH233
Plasmid#121144PurposeExpression of gRNA with BbsI placeholder for target cloningDepositorInsertgRNA cassette
UseCRISPRExpressionYeastPromoterS.pombe rrk1Available SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2
Plasmid#60388PurposeExpression of yeast beta tubulin (TUB2) using a GAL promoterDepositorInsertTUB2
ExpressionYeastPromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
7859 pRS305-pUG72
Plasmid#10897DepositorAvailable SinceApril 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pXP122
Plasmid#26827DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCfB2990
Plasmid#63645PurposepCfB2990 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastPromoternoneAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPS2169
Plasmid#1364DepositorAvailable SinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pUDP044
Plasmid#101168PurposepUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)DepositorInsertpolycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
UseCRISPRExpressionYeastPromoterScTDH3Available SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJT10R
Plasmid#98307PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3AcNES1>TRPL41B
ExpressionYeastMutationERG20 N127WAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDIV490
Plasmid#177698PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRHR2p (Debaryomyces hansenii ) and TDH3p (Candidaā¦Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKT0211
Plasmid#8734DepositorInsertyECitrine A206R
TagsCodon optimized linkerExpressionYeastMutationyEGFP with S65G, V68L, Q69M, S72A, T203Y, A206RAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pICE TRP1-KanMx
Plasmid#122902PurposeTRP1-KanMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pATP416-ĀpluxO5-ĀptetO7-ĀcrtYBI
Plasmid#165975PurposeExpresses carotenoid biosynthesis gene in response to homoserine lactone and doxycycline in yeast expressing LuxTA and rtTADepositorInsertsXanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
BTS1 (BTS1 Budding Yeast)
UseSynthetic BiologyExpressionYeastMutationC1281A, G1698A and C66A, C1359TPromoterSynthetic promoter (pluxO5), Synthetic promoter (ā¦Available SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK415-ĀphlTA2-Ā1E
Plasmid#165970PurposeExpresses phlTA(2-Ā1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of Vā¦ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimiā¦PromoterpPGK1 mutantAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
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GAL FLAG72QDProCFPp303
Plasmid#15586DepositorInserthtt 72Q Delta Pro (HTT Human)
TagsCFP and FLAGExpressionYeastMutationProline-rich region removedAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
UASGAL-CU2-Pleum
Plasmid#60328PurposeContains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promoteā¦ExpressionYeastAvailable SinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
GTU-g
Plasmid#81094PurposeGene Tagging vector URA/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)DepositorInsertsfGFP
UseCre/LoxTagssfGFPExpressionYeastAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRG221
Plasmid#64520PurposeEpisomal E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB6679
Plasmid#106158PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6638, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4I
Plasmid#104504PurposeUsed to evaluate the expression output of Saccharomyces kudriavzevii GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSkGAL1 promoter-yEGFP
ExpressionYeastAvailable SinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4Q
Plasmid#104512PurposeUsed to evaluate the expression output of Saccharomyces kudriavzevii GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSkGAL2 promoter-yEGFP
ExpressionYeastAvailable SinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV0586
Plasmid#133473PurposeIntegration into fission yeast genome at lys3 locus with bsdMX selection cassetteDepositorTypeEmpty backboneExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW mScarlet-T2A-mTurquoise2
Plasmid#118431PurposeProduces equimolar expression of mScarlet and mTq2DepositorInsertmScarlet-T2A-mTurquoise2
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKT-mCitrineV163A-HIS
Plasmid#64685PurposepKT vector encoding yeast-codon optimized yellow fluorescent protein mCitrine V163A. The V163A mutation confers fast protein maturation.DepositorInsertmCitrine V163A
ExpressionYeastMutationValine 163 changed to alanineAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal-RNQ1
Plasmid#18684DepositorInsertRnq1 (RNQ1 Budding Yeast)
ExpressionYeastAvailable SinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only