8,265 results
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Plasmid#15596DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only
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pGBW-m4046475
Plasmid#145531PurposeYeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)DepositorInsertSARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
TagsCleavable thrombin;StrepIIExpressionYeastMutationwtPromoterpGAL1Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
416Gal-FUS-H517Q-YFP
Plasmid#29620DepositorAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3
Plasmid#65451PurposepILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.DepositorTypeEmpty backboneTagsyEGFP reporterExpressionYeastAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pITGFP3
Plasmid#65452PurposepITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.DepositorTypeEmpty backboneTagsyEGFP-CLN2PEST reporterExpressionYeastAvailable SinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
FRP2002
Plasmid#159311PurposeYeast tagging vector to create gene fusion with yeast codon optimized mTurquoise2 under KanMX selectionDepositorTypeEmpty backboneExpressionYeastAvailable SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG425GAL-ccdB-DsRed
Plasmid#14369DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pWS627
Plasmid#123064PurposeG___ bicistronic proteinDepositorInsertSte4-2A-Ste18 [3-4a]
ExpressionYeastAvailable SinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB5935
Plasmid#106128PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntA_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSJD001
Plasmid#158589PurposePex22(1-36)-mRuby2 peroxisome markerDepositorInsertPex22(1-36)-mRuby2
ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB305-K3L-vac-HA
Plasmid#20054DepositorInsertK3L
TagsHAExpressionYeastAvailable SinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-ECFP-ccdB
Plasmid#14299DepositorTypeEmpty backboneUseGateway destinationTagsECFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pVW284
Plasmid#139494PurposeExpression vector for the PP7-GFP protein in S. cerevisiaeDepositorInsertpADH1 PP7∆FG-GFPenvy tCYC1
ExpressionYeastMutation∆FGPromoterpADH1Available SinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVW300
Plasmid#139495PurposeExpression vector for the PP7-GFP protein in S. cerevisiaeDepositorInsertpTEF PP7∆FG-GFPenvy tCYC1
ExpressionYeastMutation∆FGPromoterpTEFAvailable SinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
GPD 25QDProGFP p416
Plasmid#15568DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAG423GPD-ccdB-DsRed
Plasmid#14366DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastAvailable SinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
AtCOPT1 upGFP
Plasmid#117899Purposeprotein expressionDepositorAvailable SinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKT-SCFP3A-HIS
Plasmid#64686PurposepKT vector encoding yeast-codon optimized and fast-folding cyan fluorescent protein SCFP3A.DepositorInsertSCFP3A
ExpressionYeastMutationNucleotide sequence has been yeast-codon optimize…Available SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD231
Plasmid#69193PurposeIntegration of 2GFP tag at Atg31 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg31-2EGFP
Tags2xEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHB4
Plasmid#53462PurposeThis plasmid contains an ADHpr driven chimeric protein with SNC1, N-terminal GFP and C-terminal SUC2. It contains flanking homology to SUC2, allowing optional integration.DepositorInsertyeGFP-Snc1-Suc2
ExpressionYeastPromoterADHAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1TCbt
Plasmid#44515DepositorInsertsUseSynthetic Biology; Expression regulator/reporterExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable SinceApril 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRG225
Plasmid#64526PurposeEpisomal E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
p42Nat 3-F GW
Plasmid#58557PurposeMulticopy with clonNat resistance marker for triple Gateway insertionDepositorTypeEmpty backboneExpressionYeastAvailable SinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG423GAL-ccdB-EYFP
Plasmid#14221DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-CaURA3
Plasmid#162461PurposeDNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaURA3DepositorInsert3FLAG-Mnase
ExpressionYeastAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-CaHIS1
Plasmid#162462PurposeDNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-Ca HIS1DepositorInsert3FLAG-Mnase
ExpressionYeastAvailable SinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
LHP424-YCplac33-STE2-WT
Plasmid#32171DepositorAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
p413GAL1-Zipp.-L-IFP-F[2] (Yeast)
Plasmid#52893PurposePlasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[2]
TagsL-IFP-F[2]ExpressionYeastPromoterGAL1Available SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pINER2R
Plasmid#185882PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (11 copy).DepositorInsertPRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B>RPL25(partial; Arm2)
ExpressionYeastMutationNoneAvailable SinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis
Plasmid#60390PurposeExpression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoterDepositorInsertTUB1 (TUB1 Budding Yeast)
ExpressionYeastMutationinternal 6xHis (see supplement figure 4)PromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-S-tag-hphMX4
Plasmid#20767DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable SinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-ccdB-TAP
Plasmid#14268DepositorTypeEmpty backboneUseGateway destinationTagsTAPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAH1
Plasmid#41825PurposeUsed as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for hygromycin resistance.DepositorTypeEmpty backboneUsePcr templateExpressionBacterial and YeastPromoterT7, TEFAvailable SinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFS118
Plasmid#12378DepositorInsertura4, thymidine kinase
ExpressionYeastAvailable SinceSept. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRS316-GFP-COF1
Plasmid#31843DepositorAvailable SinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pXP412
Plasmid#26840DepositorTypeEmpty backboneExpressionYeastAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pYC44
Plasmid#63903PurposeInitial Integrative VectorDepositorTypeEmpty backboneTagsFRT::3'UTRcta::NAT::FRTExpressionYeastAvailable SinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
416Gal-FUS-R521H-YFP
Plasmid#29624DepositorAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc-oDam_f_GFPoNLS
Plasmid#85818PurposeiDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc oDam_f_GFPoNLS
TagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A. Codon optimization compatible to work in M…Available SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-CERT PH domain
Plasmid#58725PurposeExpresses GFP-Tagged CERT PH domain in yeastDepositorInsertcollagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
TagsGFP and MycExpressionYeastPromoterPHO5Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCfB2802
Plasmid#63655PurposepCfB2802 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxExpressionYeastPromoterTEF1 promoterAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
FRP1432
Plasmid#64509PurposeExpresses destabilized Citrine in yeast.DepositorInsertCitrine-ADH1-tail
ExpressionYeastPromoterTDH3Available SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
P413TEF-PYK1
Plasmid#34605DepositorInsertPYK1
ExpressionYeastPromoterTEFAvailable SinceJan. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pML104-kanR1136
Plasmid#162040PurposeExpresses Cas9 and contains kanR1136 guide which targets the kanR geneDepositorInsertkanR1136 guide
UseCRISPRExpressionYeastAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-dCas9-SH3
Plasmid#79613PurposeExpresses dCas9-SH3 in yeast cellsDepositorInsertSpCas9
TagsSH3 domainExpressionYeastMutationD10A and H840A for nuclease deficient Cas9PromoterpGal1Available SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBDU-Atg17
Plasmid#121394PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastPromoterADH1 promoterAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
PP363
Plasmid#78984Purpose255B. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses rHGH and IFNa2b.DepositorInsertsrHGH
IFNa2b
ExpressionYeastAvailable SinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCUP1-NMGR
Plasmid#1211DepositorAvailable SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRS403-PGAL1-weakSC_GFP
Plasmid#22315DepositorInsertGFP-rad9
UseIntegrating plasmidExpressionYeastAvailable SinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHLK
Plasmid#64182Purposeharbours marker genes HIS3, LEU2 and LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only