12,973 results

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pAAV/mIba1.GFP.miR-9.T.miR-129-2-3p.T.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA

Plasmid

#226475

Purpose

Improved microglia-targeted transgene expression

Depositor

Hirokazu Hirai

Article

Aoki et al Cell Rep Methods. 2025 Jul 25:101116. do

Inserts

mIba1 promoter, 1.7-kb, microglia-specific promoter
target sequences for miR-9x4 & miR-129-2-3px4 *2
EGFP

Use

AAV

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pv6_TAF3_2xPhd

Plasmid

#179399

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of TAF3_2xPhd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

TAF3_2xPhd

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pNoROS-CAT

Plasmid

#170152

Purpose

Mammalian expression vector encoding a constitutive nucleolar targeted roGFP and a doxycycline inducible nucleolar targeted catalase. Used to study nucleolar oxidation

Depositor

Dimitri Pestov

Article

Sapio et al Front Mol Biosci. 2021 Apr 26;8:678488.

Inserts

catalase (Cat Mouse)
roGFP2

Use

Genome integration via sleeping beauty transposon…

Tags

FLAG tag and Nucleolar Localization Sequence (NoL…

Expression

Mammalian

Promoter

RPBSA and TRE promoter

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-mGSDMD

Plasmid

#111560

Purpose

Express mouse GSDMD in E.coli

Depositor

Hongbo Luo

Article

Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

Insert

Gasdermin D (Gsdmd Mouse)

Tags

His, SUMO

Expression

Bacterial

Promoter

T7

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pSCAR_Cas9-blast_GFP

Plasmid

#162074

Purpose

3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Inserts

Cas9-2a-blasticidin S deaminase
EGFP

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Mutation

codon-optimized for expression in M. musculus

Promoter

EF1a and sv40

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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mPiezo2-CMV-Sport6

Plasmid

#81073

Purpose

mouse Piezo2

Depositor

Ardem Patapoutian

Article

Coste et al Nat Commun. 2015 May 26;6:7223. doi: 10.

Insert

Piezo2 (please see depositor comments below) (Piezo2 Mouse)

Expression

Mammalian

Available Since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV FLEX Synaptophysin GFP

Plasmid

#137188

Purpose

Cre-dependent expression of synaptophysin-GFP

Depositor

Matthew Nolan

Article

Surmeli et al Neuron. 2016 Nov 23;92(4):929. doi: 10.1

Insert

Synaptophysin GFP (Syp Mouse)

Use

AAV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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G5-CD40

Plasmid

#121166

Purpose

pMP71 vector expressing GFP - P2A - G5 - myc - CD40

Depositor

Gabriel Victora

Article

Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

Insert

G5-CD40

Use

Retroviral

Tags

GFP and myc

Expression

Mammalian

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pHIV-EF1a-2A-Ovalbumin(dN)-ZsGreen

Plasmid

#231986

Purpose

Empty backbone for expression of Ovalbumin with Bi-cistronic expression ZsGreen

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

OVA-2A-ZsGreen

Use

Lentiviral

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pv6_CBX7_2xChromo

Plasmid

#179396

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromo

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_2xChromo

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

G113V in flexible linker

Promoter

CAGGS

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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sAB-K29

Plasmid

#204735

Purpose

synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin

Depositor

Minglei Zhao

Article

Yu et al Nat Chem Biol. 2021 Aug;17(8):896-905. d

Insert

synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pv6_CBX1_2xChromo

Plasmid

#179393

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX1_2xChromo

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_MBD1_MBD

Plasmid

#179404

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MBD1_MBD

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-NL1(-)

Plasmid

#15260

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Neuroligin 1 (Nlgn1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pT5_TRE-ARID1A-FLAG_PGK-H2B-RFP

Plasmid

#203965

Purpose

Dox inducible FLAG tag ARID1a in a sleeping beauty transposon construct

Depositor

Elaine Fuchs

Article

Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

Insert

ARID1A (Arid1a Mouse)

Use

Sleeping beauty transposon

Tags

FLAG

Expression

Mammalian

Promoter

TRE

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-shRab27a

Plasmid

#120930

Purpose

conditionally knockdown Rab27a in mouse cell lines

Depositor

Mien-Chie Hung

Article

Yang et al Cell Res. 2018 Aug;28(8):862-864. doi: 1

Insert

shRab27a (Rab27a Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-ms-Sox3

Plasmid

#50779

Purpose

retroviral expression of mouse Sox3

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Sox3 (Sox3 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pDB-His-MBP-mGSDMD

Plasmid

#123365

Purpose

Expression of full-length mouse GSDMD in E. coli

Depositor

Hao Wu

Article

Orning et al Science. 2018 Nov 30;362(6418):1064-1069

Insert

GSDMD (Gsdmd Mouse)

Tags

His6-MBP

Expression

Bacterial

Promoter

T7

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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Tgfb-Induced-Enhancer:Beta-globin-minimal-promoter-EGFP:pA pDestTol2pA2

Plasmid

#220508

Purpose

TGFb-inducible fluorescent reporter (TIE:EGFP)

Depositor

Leonard Zon

Inserts

TGFb inducible enhancer
beta-globin minimal promoter
SV40 PolyA signal

Use

Zebrafish expression (tol2)

Tags

EGFP

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMK451 (BSD-P2A-dTAG)

Plasmid

#214381

Purpose

dTAG for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BSD-P2A-dTAG

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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mRuby2-MannII-N-10

Plasmid

#55903

Purpose

Localization: Mannosidase/Golgi, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

MannII

Tags

mRuby2

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
tetO-HyperTRIBE-adar_deaminase-V5-t2a-mCherry

Plasmid

#241314

Purpose

inducible mouse Adar Deaminase domain (HyperTRIBE) for expression in mammalian cells

Depositor

Stavros Lomvardas

Insert

Adar deaminase domain (Adar Mouse)

Tags

V5

Expression

Mammalian

Mutation

E957Q

Promoter

tetO and CMV

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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PB-TRE-dCas9-VPR

Plasmid

#63800

Purpose

SP-dCas9-VPR with doxycycline-inducible expression

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

dCas9-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pTet-O-Ngn2-puro

Plasmid

#52047

Purpose

3rd generation lentiviral vector, expresses Ngn2 and puromycin resistent gene under control of TetON promoter

Depositor

Marius Wernig

Article

Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

Insert

Ngn2 (Neurog2 Mouse)

Use

Lentiviral

Tags

T2A-and puromycin resistant cassette

Expression

Mammalian

Promoter

tet-on

Available Since

April 11, 2014

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

Plasmid

#67974

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2ABFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PER2:Luc Plasmid

Plasmid

#206875

Purpose

Per2 promoter luciferase reporter

Depositor

Michelle Farkas

Article

Lin et al Integr Cancer Ther. 2019 Jan-Dec;18:1534

Insert

Period 2 (Per2 Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCE-mp53DD

Plasmid

#41856

Purpose

Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

Trp53 (Trp53 Mouse)

Expression

Mammalian

Mutation

Δ40-903

Promoter

CAG

Available Since

July 18, 2013

Availability

Academic Institutions and Nonprofits only

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tetO.Nfib.Hygro

Plasmid

#117271

Purpose

Expresses Nfib and hygromycin resistance gene under control of tetO sequence.

Depositor

Henrik Ahlenius

Article

Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

Insert

Nfib (Nfib Mouse)

Use

Lentiviral

Tags

IRES and hygromycin resistant cassette (C termina…

Expression

Mammalian

Promoter

tet-on

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIPZ shNS

Plasmid

#127696

Purpose

Doxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouse

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shNS

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
DLX2-hygro

Plasmid

#97330

Purpose

Generating lentiviruses to express Dlx2 and hygromycin resistance

Depositor

Marius Wernig

Article

Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

Insert

DLX2 (Dlx2 Mouse)

Use

Lentiviral

Promoter

TetO

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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12,973 results