2,226 results

1. pMRX-IP-GFP-LC3-RFP

   Plasmid

   #84573

   Purpose

   Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Retroviral

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRX-IP-GFP-LC3-RFP-LC3ΔG

   Plasmid

   #84572

   Purpose

   Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Retroviral

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pXL010-Wnt dual (GFP-Fire) reporter

   Plasmid

   #40588

   Depositor

   Sean Palecek

   Article

   Azarin et al Biomaterials. 2012 Mar;33(7):2041-9. Epu

   Insert

   Wnt reporter sequences
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Wnt promoter

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAC-ie1-DsRed-ObirOrco-QF2-15xQUAS-GCaMP6s

   Plasmid

   #200400

   Purpose

   Expresses DsRed broadly, expresses QF2 and GCaMP6s in OSNs of the clonal raider ant

   Depositor

   Daniel Kronauer

   Article

   Hart et al Cell. 2023 Jul 6;186(14):3079-3094.e17.

   Inserts

   Discosoma red fluorescent protein
   Q factor 2
   GCaMP6s
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   15x QUAS, ObirOrco (Oocearaea biroi Orco promoter…

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-FLAG-S6K1

   Plasmid

   #100509

   Purpose

   Expresses FLAG tagged S6K1 in mammalian cells

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   S6K1 (Rps6kb1 Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA 3.1 AnxA6

   Plasmid

   #20407

   Depositor

   Carlos Enrich

   Article

   Vila de Muga et al Oncogene. 2009 Jan 22. 28(3):363-77.

   Insert

   AnxA6 (Anxa6 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2009

   Availability

   Academic Institutions and Nonprofits only

7. CaMKII-pHluorin-Synaptotagmin1

   Plasmid

   #224430

   Purpose

   Expression of Synaptotagmin1-pHluorin for optical monitoring of synaptic vesicle cycling in neurons

   Depositor

   Jaime de Juan-Sanz

   Article

   Pascual-Caro et al PLoS Biol. 2024 Oct 28;22(10):e3002860.

   Insert

   Synaptotagmin1-pHluorin
   

   Use

   Lentiviral

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available since

   July 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-GFP-LC3-RFP-LC3ΔG

   Plasmid

   #168997

   Purpose

   Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ptfLC3

   Plasmid

   #21074

   Purpose

   Mammalian expression of rat LC3 fused to mRFP and EGFP

   Depositor

   Tamotsu Yoshimori

   Article

   Kimura et al Autophagy. 2007;3(5):452-60.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   EGFP and mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LAMP1-SBP-GFP

    Plasmid

    #120172

    Purpose

    Expresses a chimera of LAMP1, SBP tag and GFP (fluorescent tag)

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    Lysosomal-associated membrane protein 1 (Lamp1 Rat)
    

    Use

    Tags

    SBP and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-C1-PKCgamma-C1A

    Plasmid

    #21205

    Depositor

    Tobias Meyer

    Article

    Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

    Insert

    PKCgamma-C1A (Prkcg Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PKCgamma-C1A domain. (Lab plasmid WC 0018)

    Promoter

    Available since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-SypHluorin 4x (S4x)

    Plasmid

    #37005

    Depositor

    Yongling Zhu, Stephen Heinemann

    Article

    Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

    Insert

    SypHluorin 4x (Syp Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Four copies of pHluorin inserted at site 184-185

    Promoter

    CMV

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1-mTagBFP2-rMPC1 shRNA

    Plasmid

    #229016

    Purpose

    Expression of an shRNA construct that knocks down rat MPC1. This plasmid also encodes a blue fluorescent protein tag to verify transfection

    Depositor

    Ghazaleh Ashrafi

    Article

    Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

    Insert

    MPC1 (Mpc1 Rat)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-mCherry-Cre-blast

    Plasmid

    #179390

    Purpose

    Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

    Insert

    mCherry (P2A) Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. CaVα2δ1

    Plasmid

    #26575

    Depositor

    Diane Lipscombe

    Article

    Lin et al J Neurophysiol. 2004 Nov . 92(5):2820-30

    Insert

    Cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Cavb3

    Plasmid

    #26574

    Depositor

    Diane Lipscombe

    Insert

    calcium channel beta subunit-III (Cacnb3 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSEP-LAMP1-RFP

    Plasmid

    #167970

    Purpose

    Expresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFP

    Depositor

    Juan Bonifacino

    Article

    Farias et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Tags

    SEP and mRFP

    Expression

    Mammalian

    Mutation

    D50E

    Promoter

    CMV

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. CGBE1-NG (pBM1246)

    Plasmid

    #140256

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_NG-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pClneoMyc rat S-SCAM alpha

    Plasmid

    #40213

    Depositor

    Yoshimi Takai, Yutaka Hata

    Article

    Hirao et al J Biol Chem. 1998 Aug 14;273(33):21105-1

    Insert

    S-SCAM (Magi2 Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

20. LAP2 Full I pAcGFP-N1 monomeric GFP (1317)

    Plasmid

    #62044

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    LAP2beta (Tmpo Rat)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGP-CMV-NES-jRGECO1a

    Plasmid

    #61563

    Purpose

    Red fluorescent calcium sensor protein

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Insert

    NES-jRGECO1a
    

    Use

    Tags

    nuclear export signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TLCV2-APOBEC1-YTH

    Plasmid

    #178949

    Purpose

    Lentiviral vector for dox-inducible expression of APOBEC1-YTH in mammalian cells

    Depositor

    Kate Meyer

    Article

    Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

    Insert

    APOBEC1-YTH-T2A-GFP
    

    Use

    Lentiviral and Synthetic Biology; Inducible

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pORANGE GFP-Grin2a KI

    Plasmid

    #131486

    Purpose

    Endogenous tagging of GluN2a: N-terminal (amino acid position: A25)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Grin2a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pORANGE GFP-Grin2b KI

    Plasmid

    #131487

    Purpose

    Endogenous tagging of GluN2b: N-terminal (amino acid position: S34)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Grin2b Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GFP-C1-CAMKIIalpha

    Plasmid

    #21226

    Depositor

    Tobias Meyer

    Article

    Shen et al Neuron. 1998 Sep . 21(3):593-606.

    Insert

    CAMKIIalpha (Camk2a Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Lab plasmid KS 0001.

    Promoter

    Available since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3/F-tractin=mStayGold

    Plasmid

    #212019

    Purpose

    For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    F-tractin
    

    Use

    Tags

    mStayGold

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TLCV2-APOBEC1-YTHmut

    Plasmid

    #178950

    Purpose

    Lentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cells

    Depositor

    Kate Meyer

    Article

    Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

    Insert

    APOBEC1-YTHmut-T2A-GFP
    

    Use

    Lentiviral and Synthetic Biology; Inducible

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CMV-mEGFP-CREB

    Plasmid

    #137001

    Purpose

    mEGFP-CREB donor for G-CREB

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

    Insert

    CREB1 (Creb1 Rat)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CMV-mCherry-KIX-mCherry

    Plasmid

    #137004

    Purpose

    mCherry-KIX acceptor for G-CREB

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

    Insert

    KIX domain (Crebbp Rat)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Z-lock cofilin

    Plasmid

    #141137

    Purpose

    controlling the actin-severing ability of cofilin with light

    Depositor

    Klaus Hahn

    Article

    Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

    Insert

    Zdk2, Cofilin, LOV2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mCherry, Zdk2(132F), Cofilin S3A, LOV WT

    Promoter

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge