2,569 results

1. pRL-SV40P

   Plasmid

   #27163

   Purpose

   Renilla SV40 promoter (no enhancer) reporter gene for normalization of luciferase experiments

   Depositor

   Ron Prywes

   Article

   Chen et al Mol Cell Biol. 1999 Jul . 19(7):4695-702

   Insert

   SV40 promoter
   

   Use

   Luciferase

   Tags

   Renilla Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. M50 Super 8x TOPFlash

   Plasmid

   #12456

   Purpose

   Beta-catenin reporter. TCF/LEF sites upstream of a luciferase reporter.

   Depositor

   Randall Moon

   Article

   Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

   Insert

   TCF/LEF binding sites (Ctnnb1 )
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. M51 Super 8x FOPFlash (TOPFlash mutant)

   Plasmid

   #12457

   Purpose

   Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

   Depositor

   Randall Moon

   Article

   Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

   Insert

   mutant TCF/LEF binding sites (Ctnnb1 )
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   mutant TCF/LEF binding sites

   Promoter

   Available since

   March 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3 Basic Vector

   Plasmid

   #212936

   Purpose

   Firefly luciferase vector for investigating regions controlling transcription

   Depositor

   Debrya Groskreutz

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Jan. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pSF3-ultraID

   Plasmid

   #172878

   Purpose

   expresses ultraID in mammalian cells with the tet-on or tet-off system

   Depositor

   Julien Béthune

   Article

   Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

   Insert

   ultraID
   

   Use

   Luciferase and Retroviral; To be used in a tet-on…

   Tags

   myc

   Expression

   Mammalian

   Mutation

   fragment ([aa2-171]) of aquifex aeolicus BirA wit…

   Promoter

   TRE-cmv

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3X ERE TATA luc

   Plasmid

   #11354

   Purpose

   Luciferase reporter containing three copies of vitellogenin Estrogen Response Element

   Depositor

   Donald McDonnell

   Article

   Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

   Insert

   ERE
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. PPRE X3-TK-luc

   Plasmid

   #1015

   Purpose

   PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

   Depositor

   Bruce Spiegelman

   Article

   Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

   Insert

   PPRE
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. MI-Luciferase-IRES-mCherry

   Plasmid

   #75020

   Purpose

   Encodes the expression of Luciferase and mCherry

   Depositor

   Xiaoping Sun

   Article

   Mallampati et al Blood. 2014 Jun 26;123(26):4064-76. doi:

   Inserts

   firefly Luciferase
   mCherry
   

   Use

   Luciferase and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-FLuc

   Plasmid

   #170575

   Purpose

   Express firefly luciferase. Used in MLV-based SARS-CoV-2 pseudovirus assay.

   Depositor

   David Nemazee

   Article

   Rogers et al Science. 2020 Aug 21;369(6506):956-963.

   Insert

   Luc
   

   Use

   Luciferase and Retroviral

   Tags

   NA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTXTL-T7max-FLuc

    Plasmid

    #188311

    Purpose

    Expression of Firefly luciferase via a T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    Firefly luciferase
    

    Use

    Luciferase and Synthetic Biology

    Tags

    8x His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7Max

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. circRNA-synIRES-R25-NanoLuc

    Plasmid

    #188116

    Purpose

    Template plasmid to transcribe circular RNA encoding NanoLuc

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    NanoLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. p10xUAS-luc2

    Plasmid

    #173006

    Purpose

    firefly luciferase reporter vector with 10x clustered UAS binding sites

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    10xUAS-luc2
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. 4xCSL-luciferase

    Plasmid

    #41726

    Depositor

    Raphael Kopan

    Article

    Saxena et al J Biol Chem. 2001 Oct 26;276(43):40268-7

    Insert

    4xCSL binding sites
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains a multimerized high affinity CSL binding…

    Promoter

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. 4xM67 pTATA TK-Luc

    Plasmid

    #8688

    Depositor

    Jim Darnell

    Article

    Besser et al Mol Cell Biol 1999 Feb;19(2):1401-9.

    Insert

    4 repeated M67 sites
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4.23-5xE-box

    Plasmid

    #206841

    Purpose

    The plasmid contains a 312 bp DNA fragment that contained 5 canonical E-boxes (GCCACGTGCA) spaced by 50 nucleotides and cloned into pGL4.23[luc2/minP] (XhoI/HindIII).

    Depositor

    Tassula Proikas-Cezanne

    Article

    Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

    Insert

    5x E-box ((GCCACGTGCA)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pF CAG luc IRES neo

    Plasmid

    #98294

    Purpose

    For constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance gene

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Inserts

    Firefly luciferase
    IRES
    Neomycin resistance gene (NeoR)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL4.10-Claudin-1

    Plasmid

    #46387

    Purpose

    Claudin-1 promoter reporter assay

    Depositor

    David Mu

    Article

    Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

    Insert

    CLDN1 Promoter (CLDN1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    CLDN1 promoter

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pMPRAdonor2

    Plasmid

    #49353

    Purpose

    minP-luc2 donor plasmid for MPRA library construction

    Depositor

    Tarjei Mikkelsen

    Article

    Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

    Insert

    luc2
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    minP

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMPRAdonor1

    Plasmid

    #49352

    Purpose

    Promoterless luc2 donor plasmid for MPRA library construction

    Depositor

    Tarjei Mikkelsen

    Article

    Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

    Insert

    luc2
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL3-E-cadherin promoter

    Plasmid

    #61798

    Purpose

    Luciferase reporter containing the mouse E-cadherin promoter

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    -178/+92 fragment of the mouse Cdh1 promoter (Cdh1 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pMPRAdonor3

    Plasmid

    #49354

    Purpose

    pSV40mod-luc2 donor plasmid for MPRA library construction

    Depositor

    Tarjei Mikkelsen

    Article

    Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

    Insert

    luc2
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    SV40mod

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. pME-Luciferase-P2A-H2A-mCherry (JDW 926)

    Plasmid

    #224488

    Purpose

    Gateway compatible middle entry clone containing a Luciferase P2A H2A-mCherry dual reporter (Luciferase/nuclear red fluorescent reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase-P2A-H2A-mCherry
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HRE-luciferase

    Plasmid

    #26731

    Purpose

    Luciferase reporter construct containing three hypoxia response elements (24-mers) from the Pgk-1 gene.

    Depositor

    Navdeep Chandel

    Article

    Emerling et al Proc Natl Acad Sci U S A. 2008 Feb 19. 1

    Insert

    Hypoxia Responsive Elements (3)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

    Plasmid

    #119816

    Purpose

    3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells

    Depositor

    Ethan Abel

    Article

    Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:

    Inserts

    Luciferase
    Puromycin
    

    Use

    Lentiviral and Luciferase

    Tags

    EGFP and V5

    Expression

    Mammalian

    Mutation

    Stop codon removed in the frame of the V5 frame v…

    Promoter

    CMV/TO and Human Phosphoglycerate kinase

    Available since

    Jan. 2, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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25. pGL4.27_ARE/NRF2-SPE

    Plasmid

    #177775

    Purpose

    Luciferase reporter for ARE/NRF2

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    NRF2 (NFE2L2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 8xGTIIC-luciferase

    Plasmid

    #34615

    Purpose

    YAP/TAZ-responsive synthetic promoter driving luciferase expression

    Depositor

    Stefano Piccolo

    Article

    Dupont et al Nature. 2011 Jun 8;474(7350):179-83. doi

    Insert

    synthetic TEAD luciferase reporter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGL3-NFAT luciferase

    Plasmid

    #17870

    Purpose

    3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue

    Depositor

    Jerry Crabtree

    Article

    Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.

    Insert

    3x NFAT binding sequence (IL2 )
    

    Use

    Luciferase

    Tags

    IL2 promoter and Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    IL-2 promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLumi-LgBiT

    Plasmid

    #188319

    Purpose

    Expression of LgBiT via sigma 70 promoter in E. coli

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    LgBiT
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    sigma 70 promoter

    Available since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLEX-307_LgBiT

    Plasmid

    #191211

    Purpose

    Lentiviral-mediated stable expression of LgBiT for slit nanoluciferase complementation assays in mammalian cells

    Depositor

    Mohan Babu

    Insert

    LgBiT (large bit)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    AB030246.1 (mutated in PMID 26569370)

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pIS1

    Plasmid

    #12179

    Depositor

    David Bartel

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge