We narrowed to 67 results for: Geobacillus
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Plasmid#44009DepositorInsertsurT-PsurP-agaN
ExpressionBacterialMutationDeleted EcoRI site (synonymous change, C to T at …Available SinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pG1AK-PheB
Plasmid#71738PurposeModular shuttle vector for Geobacillus and E. coliDepositorInsertPheB
UseSynthetic Biology; Shuttle vectorExpressionBacterialPromoterpRplsWTAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-Geo_st
Plasmid#87700PurposeExpression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBPDepositorInsertGeoCas9
Tags10xHis-MBP-TEVExpressionBacterialAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-NLS-Geo_st
Plasmid#87703PurposeExpression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLSDepositorInsertGeoCas9
Tags10xHis-MBP-TEVExpressionBacterialAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK131
Plasmid#71548PurposeProduces Geobacillus stearothermophilus (Bacillus stearothermophilus) alanine racemase (alr)DepositorInsertAlanine racemase
ExpressionBacterialPromoterT7Available SinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pG1K
Plasmid#71741PurposeModular shuttle vector for Geobacillus and E. coliDepositorTypeEmpty backboneUseSynthetic Biology; Shuttle vectorExpressionBacterialAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pG1C
Plasmid#71740PurposeModular shuttle vector for Geobacillus and E. coliDepositorTypeEmpty backboneUseSynthetic Biology; Shuttle vectorExpressionBacterialAvailable SinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pG2K
Plasmid#71742PurposeModular shuttle vector for Geobacillus and E. coliDepositorTypeEmpty backboneUseSynthetic Biology; Shuttle vectorExpressionBacterialAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIM1773-PRHIII-sfGFP-pNW33N
Plasmid#52217PurposeConstitutive sfGFP expression plasmid for use in Geobacillus and E. coliDepositorInsertPRHIII-sfGFP
ExpressionBacterialPromoterPRHIIIAvailable SinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pG1AK
Plasmid#71736PurposeModular shuttle vector for Geobacillus and E. coliDepositorTypeEmpty backboneUseSynthetic Biology; Shuttle vectorExpressionBacterialAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pG1AK-sfGFP
Plasmid#71739PurposeModular shuttle vector for Geobacillus and E. coliDepositorInsertsfGFP
UseSynthetic Biology; Shuttle vectorExpressionBacterialPromoterpRplsWTAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pG1AK-mCherry
Plasmid#71737PurposeModular shuttle vector for Geobacillus and E. coliDepositorInsertmCherry
UseSynthetic Biology; Shuttle vectorExpressionBacterialPromoterpRplsWTAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSL5200 (pDonor_ISGst3)
Plasmid#210875PurposeHarbors a native ISGst3 transposon derived form Geobacillus stearothermophilus DSM 458DepositorInsertISGst3
ExpressionBacterialAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL0_169 [pTHT15 ORI]
Plasmid#210688PurposeLevel 0 partDepositorInsertpTHT15 ORI
UseSynthetic BiologyAvailable SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVSL10
Plasmid#201182PurposeBst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavableDepositorInsertBst polymerase large fragment (BF) F710Y/D598A
TagsHRV 3C protease cleavage site and His6ExpressionBacterialMutationF710Y D598APromoterT5-lacOAvailable SinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pThermoCas9_ctrl
Plasmid#100981PurposeExpresses ThermoCas9 and its sgRNA moduleDepositorInsertsCas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module
TagsNoneExpressionBacterialPromoterB. coagulans DSM 1 pta promoter and B. smithii xy…Available SinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTTCC
Plasmid#164264PurposeExpresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilusDepositorInsertsCas9
gRNA scaffold
ExpressionBacterialPromoterPcel and trcAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512g3.1
Plasmid#171200PurposeExpresses Br512g3.1 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
Tags8x His tag; Villin Headpiece 47 amino acid (vHP47…ExpressionBacterialPromoterT7Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512g3.2
Plasmid#171201PurposeExpresses Br512g3.2 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
Tags8x His tag; Villin Headpiece 47 amino acid (vHP47…ExpressionBacterialPromoterT7Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-FPPS
Plasmid#108952Purposeheterologous expression of FPPS of G. stearothermophilus in E. coliDepositorInsertFarnesyldiphosphat synthase (FPPS)
TagsHis6-TagExpressionBacterialAvailable SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
S7SZ03
Plasmid#163199PurposeInducible expression of UniProt identifier S7SZ03DepositorInsertS7SZ03
Tags10xHis and T7ExpressionBacterialPromoterT7Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pThermoCas9i_ctrl
Plasmid#100982PurposeExpresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA moduleDepositorInsertsCatalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module
TagsNoneExpressionBacterialMutationchanged aspartate 8 to alanine and histidine 582 …PromoterB. coagulans DSM 1 pta promoter and B. smithii xy…Available SinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-Bst LF-6xHis
Plasmid#159148Purposeexpresses His-tagged large fragment of Bst polymeraseDepositorInsertlarge fragment of Geobacillus stearothermophilus DNA polymerase I
TagsHisExpressionBacterialAvailable SinceSept. 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSL4369 (pEffector_TnpB2,ωRNA)
Plasmid#210871PurposeExpresses GstTnpB2 and native ωRNA from constitutive promotersDepositorInsertGstTnpB2
ExpressionBacterialAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512
Plasmid#161875PurposeExpresses Br512 polymerase in E.coliDepositorArticleInsertModified DNA Polymerase I Large Fragment (Br512)
Tags8x His tag; Villin Headpiece 47 amino acid (HP47)…ExpressionBacterialPromoterT7, tetPAAvailable SinceNov. 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSL4164 (pEffector_IscB,ωRNA)
Plasmid#210874PurposeExpresses GstIscB and native ωRNA from constitutive promotersDepositorInsertGstIscB
ExpressionBacterialAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL4672 (pEffector_TnpB3,ωRNA)
Plasmid#210872PurposeExpresses GstTnpB3 and native ωRNA from constitutive promotersDepositorInsertGstTnpB3
ExpressionBacterialAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLU_81
Plasmid#225782PurposeRS421 library variant 13401DepositorInsert13401_[abr]
UseSynthetic BiologyMutationN/AAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLU_99
Plasmid#225812PurposeRS421 library variant 13401DepositorInsert13401_[Himar]
UseSynthetic BiologyMutationN/AAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLU_117
Plasmid#225797PurposeRS421 library variant 13401DepositorInsert13401_[Tn5]
UseSynthetic BiologyMutationN/AAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL4673 (pEffector_TnpB4,ωRNA)
Plasmid#210873PurposeExpresses GstTnpB4 and native ωRNA from constitutive promotersDepositorInsertGstTnpB4
ExpressionBacterialAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512_Mut235
Plasmid#179278PurposeExpresses Br512_Mut235 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
Tags8x His tag; Villin Headpiece 47 amino acid (HP47)…ExpressionBacterialPromoterT7Available SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAtetO_6xHis-Bst-LF
Plasmid#145799PurposePlasmid for the aTc-inducible expression of the large-fragment of Bst DNAPDepositorInsertBst-LF
Tags6xHisExpressionBacterialPromotertet PAAvailable SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIntegrase_7
Plasmid#60578Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_7
ExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
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