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We narrowed to 278 results for: plasmid transfection

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Showing: 1 - 40 of 278 results

  4. pSFV3

    Plasmid
    #92072
    Purpose
    Empty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.
    Depositor
    Henrik Garoff
    Article
    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356
    Type
    Empty backbone
    Use
    Empty expression plasmid, for run-off transcripti…
    Available Since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92072-plasmid-map-sequence-id-181621

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  5. pONSY-mCherry

    Plasmid
    #111874
    Purpose
    This plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    mCherry
    Use
    Capsaspora owczarzaki
    Tags
    mCherry
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111874-plasmid-map-sequence-id-213482

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  6. pONSY-Venus

    Plasmid
    #111875
    Purpose
    This plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    Venus
    Use
    Capsaspora owczarzaki
    Tags
    Venus
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111875-plasmid-map-sequence-id-213485

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  7. pONSY-CoH2B:Venus

    Plasmid
    #111877
    Purpose
    This plasmid expresses Venus fluorescent protein fused to endogenous Histone H2B (H2B) gene of Capsaspora (CAOG_01818). It can be used to transfect Capsaspora cells and visualize nucleus in vivo.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    Capsaspora Histone H2B (CoH2B) fused to Venus
    Use
    Capsaspora owczarzaki
    Tags
    Venus
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111877-plasmid-map-sequence-id-213489

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  8. pHLsec-mVenus

    Plasmid
    #249358
    Purpose
    Intracellular mVenus expression, transfection control
    Depositor
    Jonathan Elegheert
    Insert
    mVenus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 25, 2026
    Availability
    Academic Institutions and Nonprofits only
    249358-plasmid-map-sequence-id-501200

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  10. pDOE20 mC VN transient

    Plasmid
    #124247
    Purpose
    Allows fluorescent (mVENUS; mCherry) co-localization of two proteins in directly-transfected plant cells.
    Depositor
    Attila Fehér
    Type
    Empty backbone
    Expression
    Plant
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    124247-plasmid-map-sequence-id-239326

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  11. pTM

    Plasmid
    #162783
    Purpose
    Protein expression in CHO cells - hTPA leader and C-terminal c-myc and 10xhis tags are coded
    Depositor
    Ivan Vorobiev
    Article
    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi
    Type
    Empty backbone
    Tags
    10xHis, c-myc, and hTPA leader
    Expression
    Mammalian
    Promoter
    CHO Elongation factor 1A
    Available Since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162783-plasmid-map-sequence-id-333802

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  12. pBD21_037

    Plasmid
    #109154
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of iRFP720
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    iRFP720
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    109154-plasmid-map-sequence-id-209237

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  13. pBD21_033

    Plasmid
    #109150
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of Sirius
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    Sirius
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    109150-plasmid-map-sequence-id-209216

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  14. pBD21_036

    Plasmid
    #109153
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of mKO2
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    mKO2
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    109153-plasmid-map-sequence-id-209235

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  15. pBD21_034

    Plasmid
    #109151
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of TagBFP
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    TagBFP
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    109151-plasmid-map-sequence-id-220104

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  16. pBD21_035

    Plasmid
    #109152
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFP
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    EGFP
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    109152-plasmid-map-sequence-id-209236

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  17. CMV-pDisplay-Halo-T2A-EGFP

    Plasmid
    #223498
    Purpose
    Co-expression of HaloTags to outer surface in mammalian cells coupled with a EGFP transfection marker
    Depositor
    James Frank
    Insert
    HaloTag and EGFP
    Expression
    Mammalian
    Available Since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    223498-plasmid-map-sequence-id-443020

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  19. hSyn-pDisplay-SNAP-T2A-EGFP

    Plasmid
    #223503
    Purpose
    Co-expression of SNAP-tags to outer surface in mammalian cells coupled with an EGFP transfection marker
    Depositor
    James Frank
    Insert
    SNAP-tag and EGFP
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    223503-plasmid-map-sequence-id-443301

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  21. p1.1-Tr2-eGFP

    Plasmid
    #162782
    Purpose
    Fluorescent reporter for protein expression studies
    Depositor
    Ivan Vorobiev
    Article
    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi
    Insert
    enhanced green fluorescent protein
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available Since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    162782-plasmid-map-sequence-id-338334

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  23. pLenti_P2A_Blast

    Plasmid
    #211365
    Purpose
    Lentiviral expression vector for an insert of interest linked to a P2A-T2A blasticidin sequence. Produces virus very efficiently. Can also be used for regular transfections
    Depositor
    Jacques Neefjes
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    211365-plasmid-map-sequence-id-437557

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  24. hSyn-pDisplay-SNAP[C145A]-T2A-EGFP

    Plasmid
    #223504
    Purpose
    Co-expression of dead-mutant SNAP-tags to outer surface in mammalian cells coupled with an EGFP transfection marker
    Depositor
    James Frank
    Insert
    dead-mutant SNAP-tag and EGFP
    Expression
    Mammalian
    Mutation
    SNAP-tag C145A
    Promoter
    hSyn
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    223504-plasmid-map-sequence-id-443304

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  27. pONSY-Lifeact:mCherry

    Plasmid
    #111876
    Purpose
    This plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    Lifeact fused to mCherry
    Use
    Capsaspora owczarzaki
    Tags
    mCherry
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available Since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111876-plasmid-map-sequence-id-213488

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  28. pTM-RBDv2

    Plasmid
    #162785
    Purpose
    SARS-CoV-2 S protein receptor binding domain (RBD) expression in mammalian cells
    Depositor
    Ivan Vorobiev
    Article
    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi
    Insert
    SARS-CoV-2 S protein receptor binding domain (RBD) (S Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
    Tags
    10xHis, c-myc, and hTPA leader
    Expression
    Mammalian
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available Since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162785-plasmid-map-sequence-id-333799

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  30. pONSY-CoNMM:mCherry

    Plasmid
    #111878
    Purpose
    This plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    Capsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
    Use
    Capsaspora owczarzaki
    Tags
    mCherry
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111878-plasmid-map-sequence-id-213490

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  31. pT2iD6Hoxa13bER4(#686)

    Plasmid
    #184101
    Purpose
    cyclofen-inducible fish Hoxa13b activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    ZfHoxa13b-ERT2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available Since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184101-plasmid-map-sequence-id-365951

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  32. pT2iD6Hoxa13aER4(#685)

    Plasmid
    #184100
    Purpose
    cyclofen-inducible fish Hoxa13a activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    ZfHoxa13a-ERT2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184100-plasmid-map-sequence-id-364020

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  34. pcDNA3.1-E-Flag

    Plasmid
    #101858
    Purpose
    Evi-1 flag tagged for transfection into mammalian cells
    Depositor
    Archibald Perkins
    Insert
    Evi-1 (Mecom Mouse)
    Tags
    Flag
    Expression
    Mammalian
    Available Since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    101858-plasmid-map-sequence-id-211082

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  35. pT2iC6Dronpa2nlsER4(#636)

    Plasmid
    #184067
    Purpose
    cyclofen-inducible Dronpa2 nuclear relocation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    Dronpa2-2nls-ERT2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184067-plasmid-map-sequence-id-363960

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  36. pT2iC6FAST1nls2ER4(#638)

    Plasmid
    #184068
    Purpose
    cyclofen-inducible YFAST nuclear relocation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    YFAST-2nls-ERT2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available Since
    June 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    184068-plasmid-map-sequence-id-365820

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  37. Clim Act MCH (CAM)

    Plasmid
    #104443
    Purpose
    Expresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.
    Depositor
    Elena Casacuberta, Inaki Ruiz-Trillo
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Promoter
    actin
    Available Since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    104443-plasmid-map-sequence-id-200869

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  38. Clim EF1 MCH (CEM)

    Plasmid
    #104444
    Purpose
    Expresses mCherry under elongation factor 1 promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.
    Depositor
    Elena Casacuberta, Inaki Ruiz-Trillo
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Promoter
    EF1
    Available Since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    104444-plasmid-map-sequence-id-200864

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  39. Clim Tub MCH (CTM)

    Plasmid
    #104445
    Purpose
    Expresses mCherry under tubulin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.
    Depositor
    Elena Casacuberta, Inaki Ruiz-Trillo
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Promoter
    tubulin promoter
    Available Since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    104445-plasmid-map-sequence-id-200865

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Showing: 1 - 40 of 278 results