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Plasmid#112127Purposeempty sgRNA cloning vector with MS2-AIDmonoDepositorInsertAID mono mutant (AICDA Human)
UseCRISPRTagsExpressionMammalianMutationN- mutated and C- terminal truncated, H130A/R131E…PromoterhU6,CBhAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL3 sgRNA(MS2)-MS2-AID3f
Plasmid#112129Purposeempty sgRNA cloning vector with MS2-AID3fDepositorInsertAIDmono 3f mutant (AICDA Human)
UseCRISPRTagsExpressionMammalianMutationN- mutated and C- terminal truncated, F115Y/C116F…PromoterhU6,CBhAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL2 sgRNA(MS2)-MS2-AID3c
Plasmid#112128Purposeempty sgRNA cloning vector with MS2-AID3cDepositorInsertAID mono 3c mutant (AICDA Human)
UseCRISPRTagsExpressionMammalianMutationN- mutated and C- terminal truncated, F115Y/C116F…PromoterhU6,CBhAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL4 sgRNA(MS2)-MS2-hA3a
Plasmid#112130Purposeempty sgRNA cloning vector with MS2-humanA3aDepositorInserthuman Apobec3a (APOBEC3A Human)
UseCRISPRTagsExpressionMammalianMutationPromoterhU6,CBhAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1
Plasmid#92120PurposeExpression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.DepositorInsertMS2-P65-HSF1 (HSF1 Human, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLL6 sgRNA(MS2)-MS2-hA3a D131E
Plasmid#112132Purposeempty sgRNA cloning vector with MS2-humanA3a D131EDepositorInserthuman Apobec3a D131E mutant (APOBEC3A Human)
UseCRISPRTagsExpressionMammalianMutationD131E mutant in hA3aPromoterhU6,CBhAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL5 sgRNA(MS2)-MS2-hA3a D131A
Plasmid#112131Purposeempty sgRNA cloning vector with MS2-humanA3a D131ADepositorInserthuman Apobec3a D131A mutant (APOBEC3A Human)
UseCRISPRTagsExpressionMammalianMutationD131A mutant in hA3aPromoterhU6,CBhAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC014_pLKO-U6-BsmBI-MS2sgRNA-EFS-Thy11-2A-MS2-p65-HSF1
Plasmid#192187PurposeTdgA vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationNAPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pMBP-MS2
Plasmid#65104Purposecan be used to isolate RNA binding complexesDepositorInsertMS2
UseTagsMBPExpressionBacterialMutationdouble mutation (V75Q and A81G) that prevents oli…PromotertacAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
prCAG-MS2
Plasmid#191121PurposeTranscription of 47xCAG and 12xMS2 repeats from a tetracycline-inducible promoterDepositorInsertsrCAG
MS2
UseSynthetic BiologyTagsExpressionMutationPromoterTetAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUB_FLuc100---MS2
Plasmid#210574PurposeExpresses Optimal Firefly with 3'UTR MS2 hairpinsDepositorInsertOptimal Firefly luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
MS2-Snapf
Plasmid#65860PurposeExpresses RNA-binding protein MS2 with fast SNAP fusion tagDepositorInsertsMS2
Fast SNAP
UseTagsHA and NLSExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
MS2-pegRNA
Plasmid#181811Purposems2 incorporated pegRNA-1.1DepositorInsertMS2-pegRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
MS2-ecDHFR
Plasmid#65859PurposeExpresses RNA-binding protein MS2 with eDHFR fusion tagDepositorInsertsUseTagsHA and NLSExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
LsgRNA-MS2
Plasmid#235597PurposesgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. The cloning site is BsmbI.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUB_SMFLuc0---MS2
Plasmid#210568PurposeExpresses SM(FLAG)-nonoptimal Firefly with 3'UTR MS2 hairpinsDepositorInsertSpaghetti monster (FLAG)-nonoptimal FLuc
UseLentiviral and LuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SMFLuc100---MS2
Plasmid#210570PurposeExpresses SM(FLAG)-optimal Firefly with 3'UTR MS2 hairpinsDepositorInsertSpaghetti monster (FLAG)-optimal FLuc
UseLentiviral and LuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_smHA_KDM5B_MS2
Plasmid#81085PurposeTranslation reporter: Encodes KDM5B (1544 aa) fused to smHA (Spaghetti Monster HA: 10 copies of an epitope tag HA).DepositorInsertsmHA_KDM5B_24xMS2
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGH224_sgRNA_2xMS2_Puro
Plasmid#85413PurposehU6 driven sgRNA vector with 2xMS2 vectors with puro selectable markerDepositorInsertpuromycin resistance
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZE31ms2
Plasmid#25860DepositorInsertms2 binding site (x2)
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceNov. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hPMS2
Plasmid#129425PurposeExpression human PMS2 protein using baculovirusDepositorInserthuman PMS2 (PMS2 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrin promoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
MS2_GFP
Plasmid#61764PurposeExpresses MS2 coat protein fused to GFPDepositorInsertMS2 coat protein fused to eGFP
UseLentiviralTagseGFPExpressionMammalianMutationPromoterUbc promoterAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUB_smFLAG_ActB_MS2
Plasmid#81083PurposeTranslation reporter: Encodes Beta-actin (374 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).DepositorInsertsmFLAG_ActB_24xMS2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pMC_MS2_DNA_VLP
Plasmid#179156PurposeExpression of maturation protein and his-tagged coat protein dimer from MS2 phageDepositorInsertsMaturation Protein
Coat Protein Dimer
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS2155
Plasmid#141065PurposeAAV plasmid with SYN1 promoter driving expression of EmGFP. Contains WPRE.DepositorInsertSYN1-EmGFP-WPRE
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailable sinceApril 28, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
HisMS2_VLP_RSV
Plasmid#175958PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the RSV P and M genes [GenBank: M74568.1]DepositorInsertsP gene
M gene
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUB_smFLAG_KDM5B_MS2
Plasmid#81084PurposeTranslation reporter: Encodes KDM5B (1544 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).DepositorInsertsmFLAG_KDM5B_24xMS2
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS2022
Plasmid#49129PurposeAAV plasmid with DBH promoter (PRS2/3:Con) driving expression of iCre.DepositorInsertssAAV-PRS2/3:Con-iCre
UseAAVTagsExpressionMammalianMutationPromoterDBHAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
Lenti_sgRNA_MS2_neo
Plasmid#118650PurposeTo clone sgRNA for activation dCas9DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterU6Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEMS2115
Plasmid#49140PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertssAAV-Ple155-emGFP-WPRE
UseAAVTagsExpressionMammalianMutationPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS2280
Plasmid#111901PurposessAAV genome with Ple345 (NEFL MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertPle345 (NEFL Human)
UseAAVTagsExpressionMutationPromoterPle345 (NEFL MiniPromoter)Available sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEMS2241
Plasmid#111896PurposessAAV genome with Ple320 (POU4F1 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertPle320 (POU4F1 Human)
UseAAVTagsExpressionMutationPromoterPle320 (POU4F1 MiniPromoter)Available sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
MS2_SC2_S_Delta
Plasmid#175950PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Delta SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationDelta (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Lenti_MS2_P65_HSF1_2A_puro
Plasmid#118699PurposeTo express MS2-P65-HSF1DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMS2-1
Plasmid#220627PurposePlasmid used to place the RNA sequence of interest next to two MS2 coat protein recognition sites.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS2-2
Plasmid#220628PurposePlasmid allows you to place the RNA sequence of interest next to two MS2 coat protein recognition sites.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAP72_MS2
Plasmid#220623PurposeCassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.DepositorTypeEmpty backboneUseShuttle vectorTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pEMS2113
Plasmid#49138PurposeAAV plasmid with FEV (Ple67) promoter driving expression of EmGFP. Contains WPRE.DepositorInsertssAAV-Ple67-emGFP-WPRE
UseAAVTagsExpressionMammalianMutationPromoterFEVAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pLEX_307_ms2_VPR
Plasmid#79376PurposeRecruits VPR to compatible gRNAs for transcriptional activationDepositorInsertms2-VPR
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS2021
Plasmid#49128PurposeAAV plasmid with DCX (Ple302) promoter driving expression of iCre.DepositorInsertssAAV-Ple302-iCre
UseAAVTagsExpressionMammalianMutationPromoterDCXAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMS26
Plasmid#32295DepositorTypeEmpty backboneUseBacterial gene additionTagsExpressionBacterialMutationPromoterrhaBAvailable sinceSept. 26, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLEX_307_ms2_p65_hsf1
Plasmid#79374PurposeRecruits p65_hsf1 to compatible gRNAs for transcriptional activationDepositorInsertms2-p65-hsf1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMS2
Plasmid#191123PurposeTranscription of 12xMS2 repeats from a tetracycline-inducible promoterDepositorInsertMS2
UseSynthetic BiologyTagsExpressionMutationPromotertetracycline-inducible promoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEMS2118
Plasmid#49143PurposeAAV plasmid with smCBA promoter driving expression of iCre. Contains WPRE.DepositorInsertssAAV-smCBA-iCre-WPRE
UseAAVTagsExpressionMammalianMutationPromotersmCBAAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
HisMS2_VLP_FluA
Plasmid#175955PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the Influenza A Matrix Protein [A/Illinois/20/2018(H1N1)]DepositorInsertM gene
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits