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Plasmid#190709PurposeEncodes the antiparallel coiled-coil apCC-Di followed by C-Venus.DepositorInsertapCC-Di-C-Venus
UseTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD/His-miRFP718nano
Plasmid#195743PurposeMonomeric near-infrared fluorescent protein miRFP718nano in bacterial plasmidDepositorInsertmiRFP718nano
UseTagsHisExpressionBacterialMutationPromoteraraCAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSB4A3-PBAD-phzO
Plasmid#165619PurposeArabinose-inducible expression of phzO for conversion of phenazine-1-carboxylic acid to 2-hydroxyphenazineDepositorInsertphzO
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB4A3-PBAD-phzSM
Plasmid#165620PurposeArabinose-inducible expression of phzSM for conversion of phenazine-1-carboxylic acid to pyocyaninDepositorUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH28_pBad_AraC_Para-sfGFP.3TAG
Plasmid#163723PurposeArabinose-inducible expression of green fluorescent protein encoding three amber codonsDepositorInsertaraC gfp.3TAG
UseTagsExpressionBacterialMutationgfp.N39TAG,N135TAG,Y151TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD/His-D-iLight
Plasmid#170270PurposeExpression of iLight with 6xHis N-terminal tag in bacteria.DepositorInsertiLight
UseTags6xHis tagExpressionBacterialMutationSee CommentsPromoteraraBADAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Citroff1-R251
Plasmid#134307PurposeBacterial expression of an inverse-response citrate sensor variant with medium binding affinityDepositorInsertCitroff1 R251A
UseTags6-His tagExpressionBacterialMutationPromoterpBADAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Citroff1-S245
Plasmid#134302PurposeBacterial expression of an inverse-response citrate sensor variant with medium binding affinityDepositorInsertCitroff1 S245A
UseTags6-His tagExpressionBacterialMutationPromoterpBADAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u36_Snifit
Plasmid#119045PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 36 (FRET semi-synthetic biosensor)DepositorInsertMTX-u36_Snifit
UseTagsHis-tag and Strep-tagExpressionBacterialMutation36TAG R44L H45QPromoterpBADAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u51_Snifit
Plasmid#119046PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 51 (FRET semi-synthetic biosensor)DepositorInsertMTX-u51_Snifit
UseTagsHis-tag and Strep-tagExpressionBacterialMutation51TAG R44L H45QPromoterpBADAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u23_Snifit
Plasmid#119044PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 23 (FRET semi-synthetic biosensor)DepositorInsertMTX-u23_Snifit
UseTagsHis-tag and Strep-tagExpressionBacterialMutation23TAG R44L H45QPromoterpBADAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBad-HPII TAG206
Plasmid#105846Purposeexpresses E. coli HPII catalase with TAG stop codon at site 206 and N-terminal 6x his tagDepositorArticleInserthydroperoxidase II catalase
UseTags6x his tagExpressionBacterialMutationTAG stop codon at site 206PromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBad-HPII TAG568
Plasmid#105845Purposeexpresses E. coli HPII catalase with TAG stop codon at site 568 and N-terminal 6x his tagDepositorArticleInserthydroperoxidase II catalase
UseTags6x his tagExpressionBacterialMutationTAG stop codon at site 568PromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBad-CAts TAG97
Plasmid#105668Purposeexpresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 97 and C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
UseTags6x his tagExpressionBacterialMutationTAG stop codon at site 97PromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
I13453 (pBAD)_AB
Plasmid#66016PurposeMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [A:I13453:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
I13453 (pBAD)_EB
Plasmid#66017PurposeMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [E:I13453:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
I13453 (pBAD)_FB
Plasmid#66018PurposeMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [F:I13453:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
I13453 (pBAD)_GB
Plasmid#66019PurposeMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [G:I13453:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-VR
Plasmid#62286Purposeexpression of VR sgRNA from the arabinose-inducible promoterDepositorInsertVR sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB (TIREVOL)-sfGFP
Plasmid#189675Purposetitratable expression of sfGFP in bacteriaDepositorInsertsfGFP
UseTagsHis-T7-EKExpressionBacterialMutationPromoterpBADAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MBP-His-ACPs
Plasmid#141140PurposeExpression of holo-[acyl-carrier-protein] synthaseDepositorInsertacpS
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceMay 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
PBAD-his6-prkA-pACYC184
Plasmid#41041DepositorInsertprkA
UseTags6xHis and T7 epitope tagExpressionBacterialMutationPromoterPBADAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-miRFP680-HO1
Plasmid#197253PurposeExpresses the protein of miRFP680 and HO1 in bacteriaDepositorInsertmiRFP680
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-miRFP720-HO1
Plasmid#197257PurposeExpresses the protein of miRFP720 and HO1 in bacteriaDepositorInsertmiRFP720
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSG25-pBAD-tetO-deGFPssrA
Plasmid#102452PurposeExpresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.DepositorInsertdeGFP
UseTagsssrAExpressionBacterialMutationPromoterpBAD-tetOAvailable sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MBP-His-SFP
Plasmid#141141PurposeExpression of 4'-phosphopantetheinyl transferase geneDepositorInsert4'-phosphopantetheinyl transferase
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceMay 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBad-Int Set Flipper
Plasmid#38209DepositorInsertBxb1 Integrase
UseSynthetic Biology; Biobrick cloning vectorTagsHisExpressionMutationPromoterpBAD/AraCAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-PhoCl-MBP
Plasmid#87689PurposeExpress PhoCl-MBP in E.coliDepositorInsertPhoCl fused to Maltose-Binding Protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-A2NT
Plasmid#62257Purposeexpression of A2NT sgRNA from the arabinose-inducible promoterDepositorInsertA2NT sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTP140 (pBAD322G_TniQ-Cascade(Tn6900))
Plasmid#164262PurposeVector expressing the TniQ, Cas8/5, Cas7, and Cas6 proteins from the Tn6900 element with an arabinose promoter.DepositorInsertCodon-optimized tniQ-cas8/5-cas7-cas6 operon from Tn6900 from A. salmonicida S44 pS44-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTP170 (pBAD33_CRISPR(Tn6900)-entry)
Plasmid#164263PurposeEntry vector for cloning user-defined spacers in the Tn6900 CRISPR array.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterArabinoseAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
araC-pBAD-gusA-pBAV1k
Plasmid#30502DepositorInsertbeta-glucuronidase (gusA Escherichia coli)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-miRFP713-HO1
Plasmid#197255PurposeExpresses the protein of miRFP713 and HO1 in bacteriaDepositorInsertmiRFP713
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB (TIREVOL)-mEos3.2
Plasmid#189720Purposetitratable expression of mEos3.2 in bacteriaDepositorInsertmEos3.2
UseTagsHis-T7-EKExpressionBacterialMutationPromoterpBADAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB (TIRSTD)-sfGFP
Plasmid#189674Purposetitratable expression of sfGFP in bacteriaDepositorInsertsfGFP
UseTagsHis-T7-EKExpressionBacterialMutationPromoterpBADAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-tdTEVDB_C-15A/G4C
Plasmid#177205PurposePermuted td intron with evolved initiation sequence for the expression of circular mRNADepositorInsertPermuted GFP with evolved initiation sequence for circular mRNA
UseTags6x HisExpressionBacterialMutationPermuted sfGFP after residue 52 & Changed Cyt…PromoteraraBADAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB (TIRSTD)-mNG
Plasmid#189721Purposetitratable expression of mNeonGreen in bacteriaDepositorInsertmNeonGreen
UseTagsHis-T7-EKExpressionBacterialMutationPromoterpBADAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-mNG-C-Kbp
Plasmid#178013PurposeBacterial expression of green fluorescent potassium indicator mNG-C-KbpDepositorInsertmNG-C-Kbp
UseTagsExpressionBacterialMutationPromoteraraBAvailable sinceDec. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-mNG-Pa-Kbp
Plasmid#178014PurposeBacterial expression of green fluorescent potassium indicator mNG-Pa-KbpDepositorInsertmNG-Pa-Kbp
UseTagsExpressionBacterialMutationPromoteraraBAvailable sinceDec. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-mNG-Hv-Kbp
Plasmid#178015PurposeBacterial expression of green fluorescent potassium indicator mNG-Hv-KbpDepositorInsertmNG-Hv-Kbp
UseTagsExpressionBacterialMutationPromoteraraBAvailable sinceDec. 2, 2023AvailabilityAcademic Institutions and Nonprofits only