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Showing: 451 - 500 of 790 results

  1. pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233)

    Plasmid
    #229841
    Purpose
    A Tol2 based expression vector with the Hsp70 promoter driving a cre dependent switch reporter. Contains an endothelial driven cre
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    loxp-H2B-mCerulean-2xStop-loxP
    Use
    Cre/Lox; Tol2 based expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229841-plasmid-map-sequence-id-461093

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  2. pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(T17N)-FKBP-mVenus-CAAX

    Plasmid
    #214279
    Purpose
    Express split-Cdc42 fragments that are fused with CIDs and FPs
    Depositor
    Cliff Stains
    Article
    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202
    Inserts
    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    Use
    Tags
    mCerulean (on insert 1) and mVenus (on insert 2)
    Expression
    Mammalian
    Mutation
    Cdc42-T17N
    Promoter
    CMV
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214279-plasmid-map-sequence-id-422248

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  3. pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(Q61L)-FKBP-mVenus-CAAX

    Plasmid
    #214280
    Purpose
    Express split-Rac1 fragments that are fused with CIDs and FPs
    Depositor
    Cliff Stains
    Article
    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202
    Inserts
    Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
    Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
    Use
    Tags
    mCerulean (on insert 1) and mVenus (on insert 2)
    Expression
    Mammalian
    Mutation
    Rac1-Q61L
    Promoter
    CMV
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214280-plasmid-map-sequence-id-422247

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  4. pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(T17N)-FKBP-mVenus-CAAX

    Plasmid
    #214281
    Purpose
    Express split-Rac1 fragments that are fused with CIDs and FPs
    Depositor
    Cliff Stains
    Article
    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202
    Inserts
    Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
    Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
    Use
    Tags
    mCerulean (N terminal on insert 1) and mVenus (on…
    Expression
    Mammalian
    Mutation
    Rac1-T17N
    Promoter
    CMV
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214281-plasmid-map-sequence-id-422246

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  5. pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(Q63L)-FKBP-mVenus-CAAX

    Plasmid
    #214282
    Purpose
    Express split-RhoA fragments that are fused with CIDs and FPs
    Depositor
    Cliff Stains
    Article
    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202
    Inserts
    Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
    ras homolog family member A (RHOA Human)
    Use
    Tags
    mCerulean (on insert 1) and mVenus (on insert 2)
    Expression
    Mammalian
    Mutation
    RhoA-Q63L
    Promoter
    CMV
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214282-plasmid-map-sequence-id-422245

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  6. pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(T19N)-FKBP-mVenus-CAAX

    Plasmid
    #214283
    Purpose
    Express split-RhoA fragments that are fused with CIDs and FPs
    Depositor
    Cliff Stains
    Article
    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202
    Inserts
    Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
    Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
    Use
    Tags
    mCerulean (N terminal on insert 1) and mVenus (on…
    Expression
    Mammalian
    Mutation
    RhoA-T19N
    Promoter
    CMV
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214283-plasmid-map-sequence-id-422243

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  7. pIRES-mCerulean-FRB-KRas/N12-KRas/13C(Q61L)-FKBP-mVenus-CAAX

    Plasmid
    #214284
    Purpose
    Express split-KRas fragments that are fused with CIDs and FPs
    Depositor
    Cliff Stains
    Article
    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202
    Inserts
    Split-KRas fragments fused with CIDs and FPs (KRAS Human)
    Split-KRas fragments fused with CIDs and FPs (KRAS Human)
    Use
    Tags
    mCerulean (on insert 1) and mVenus (on insert 2)
    Expression
    Mammalian
    Mutation
    KRas-Q61L
    Promoter
    CMV
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214284-plasmid-map-sequence-id-422242

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  8. pIRES-mCerulean-FRB-KRas/N12-KRas/13C(S17N)-FKBP-mVenus-CAAX

    Plasmid
    #214285
    Purpose
    Express split-KRas fragments that are fused with CIDs and FPs
    Depositor
    Cliff Stains
    Article
    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202
    Inserts
    Split-KRas fragments fused with CIDs and FPs (KRAS Human)
    Split-KRas fragments fused with CIDs and FPs (KRAS Human)
    Use
    Tags
    mCerulean (N terminal on insert 1) and mVenus (on…
    Expression
    Mammalian
    Mutation
    KRas-S17N
    Promoter
    CMV
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214285-plasmid-map-sequence-id-422241

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  9. pIRES-mCerulean-GID1-KRas/N12-KRas/13C(Q61L)-GAI1-92-mVenus-CAAX

    Plasmid
    #214286
    Purpose
    Express split-KRas fragments that are fused with CIDs and FPs
    Depositor
    Cliff Stains
    Article
    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202
    Inserts
    Split-KRas fragments fused with CIDs and FPs (KRAS Human)
    Split-KRas fragments fused with CIDs and FPs (KRAS Human)
    Use
    Tags
    mCerulean (N terminal on insert 1) and mVenus (on…
    Expression
    Mammalian
    Mutation
    KRas-Q61L
    Promoter
    CMV
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214286-plasmid-map-sequence-id-422240

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  10. pIRES-mCerulean-GID1-KRas/N12-KRas/13C(S17N)-GAI1-92-mVenus-CAAX

    Plasmid
    #214287
    Purpose
    Express split-KRas fragments that are fused with CIDs and FPs
    Depositor
    Cliff Stains
    Article
    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202
    Inserts
    Split-KRas fragments fused with CIDs and FPs (KRAS Human)
    Split-KRas fragments fused with CIDs and FPs (KRAS Human)
    Use
    Tags
    mCerulean (on insert 1) and mVenus (on insert 2)
    Expression
    Mammalian
    Mutation
    KRas-S17N
    Promoter
    CMV
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214287-plasmid-map-sequence-id-422239

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  11. C13m

    Plasmid
    #120960
    Purpose
    MoClo golden gate assembly CD part for Cerulean (modified CFP; variant of https://www.fpbase.org/protein/cerulean/) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Cerulean
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120960-plasmid-map-sequence-id-245422

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  18. pAAV-sCAG-GluClα.CreON

    Plasmid
    #196995
    Purpose
    Cre recombinase dependent expression of GluClv2.0 alpha subunit. GluClα contains a Cerulean tag. When co-expressed with GluClv2.0 beta subunit, agonist (Ivermectin) induces neuronal silencing.
    Depositor
    David Bennett
    Article
    Middleton et al Pain. 2023 Jun 27. doi: 10.1097/j.pain.0
    Insert
    GluClα -Cerulean
    Use
    AAV and Cre/Lox
    Tags
    Cerulean
    Expression
    Mutation
    Promoter
    short CAG
    Available since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196995-plasmid-map-sequence-id-390150

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  19. pAAV-FLEX-C-RG

    Plasmid
    #49101
    Purpose
    Cre dependent Cerulean-E2A-SADB19 rabies glycoprotein expression, under EF1a promoter, in AAV virus
    Depositor
    Troy Margrie
    Insert
    Cerulean-E2A-RG
    Use
    AAV and Cre/Lox
    Tags
    cerulean
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    49101-plasmid-map-sequence-id-91148

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  23. pBS10

    Plasmid
    #83795
    Purpose
    Useful for tagging yeast proteins with C-terminal Cerulean tag; hygromycin-B selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    Cerulean
    Use
    Tags
    Cerulean
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    83795-plasmid-map-sequence-id-160032

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  24. pCer-C3-Plk1

    Plasmid
    #68132
    Purpose
    Expression of Cerulean-tagged Plk1 in mammalian cells
    Depositor
    Catherine Lindon
    Insert
    Polo-like kinase 1 (PLK1 Human)
    Use
    Tags
    Cerulean
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CMV
    Available since
    Oct. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68132-plasmid-map-sequence-id-123472

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  25. pCer-C3-Plk1-T210D

    Plasmid
    #68133
    Purpose
    Expression of Cerulean-tagged, constitutively active Plk1 in mammalian cells
    Depositor
    Catherine Lindon
    Insert
    Polo-like kinase 1 (PLK1 Human)
    Use
    Tags
    Cerulean
    Expression
    Mammalian
    Mutation
    T210D constitutively active version
    Promoter
    CMV
    Available since
    Oct. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68133-plasmid-map-sequence-id-123512

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  26. pCer-C3-Plk1-K82R

    Plasmid
    #68134
    Purpose
    Expression of Cerulean-tagged, kinase-dead Plk1 in mammalian cells
    Depositor
    Catherine Lindon
    Insert
    Polo-like kinase 1 (PLK1 Human)
    Use
    Tags
    Cerulean
    Expression
    Mammalian
    Mutation
    K82R kinase-dead version
    Promoter
    CMV
    Available since
    Oct. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68134-plasmid-map-sequence-id-123466

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  27. pCeru-N1 cyclin A (1557)

    Plasmid
    #61842
    Purpose
    mammalian expression of cyclin A with cerulean fusion
    Depositor
    Jonathon Pines
    Insert
    cyclin A (CCNA2 Human)
    Use
    Tags
    Cerulean
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  28. Venus-30aa-Noxa-pEGFP-C1

    Plasmid
    #166762
    Purpose
    To increase the length of the linker between Venus and Noxa proteins
    Depositor
    David Andrews
    Insert
    Noxa (PMAIP1 Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166762-plasmid-map-sequence-id-328844

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  29. Venus-30aa-Noxa-4E-pEGFP-C1

    Plasmid
    #167529
    Purpose
    To increase the length of the linker between Venus and Noxa-4E proteins
    Depositor
    David Andrews
    Insert
    PMA-induced protein 1 (PMAIP1 Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    C25E, L29E, F32E, L36E
    Promoter
    CMV
    Available since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    167529-plasmid-map-sequence-id-347649

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  31. Venus-79aa-ActA-pEGFP-C1

    Plasmid
    #166763
    Purpose
    To target Venus to the mitochondrial outer membrane. ActA tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.
    Depositor
    David Andrews
    Insert
    ActA tail anchor (Listeria monocytogenes) (actA Listeria monocytogenes, Synthetic)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    166763-plasmid-map-sequence-id-328851

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  39. pCAX-SCLM

    Plasmid
    #216757
    Purpose
    For strong and long-lasting expression of SuperClomeleon in mammalian cells from the hybrid CAG promoter
    Depositor
    George Augustine
    Insert
    SuperClomeleon
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S30R and 11 AA deletion in the Cerulean CFP moiet…
    Promoter
    CAG (cytomegalovirus-chicken beta-actin-rabbit be…
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216757-plasmid-map-sequence-id-429395

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  40. Venus-7aa-Cb5-pEGFP-C1

    Plasmid
    #166764
    Purpose
    To target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments.
    Depositor
    David Andrews
    Insert
    Cb5 tail anchor (Cyb5a Rat)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166764-plasmid-map-sequence-id-328853

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  41. Venus-7aa-MAO-pEGFP-C3

    Plasmid
    #166765
    Purpose
    To target Venus to the mitochondrial outer membrane. MAO tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.
    Depositor
    David Andrews
    Insert
    MAO tail anchor (MAOA Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    166765-plasmid-map-sequence-id-328859

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  42. pMTB-Multibow-C

    Plasmid
    #60979
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic cerulean
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    Cerulean
    Use
    Zebrafish
    Tags
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    Feb. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  43. pAAV/hSyn-SCLM

    Plasmid
    #216760
    Purpose
    AAV2 transfer vector with the human synapsin I promoter for neuronal expression of SuperClomeleon
    Depositor
    George Augustine
    Insert
    SuperClomeleon
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S30R and 11 AA deletion in the Cerulean CFP moiet…
    Promoter
    hSyn (human synapsin I)
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216760-plasmid-map-sequence-id-429492

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  44. pAAV/EF1a-DIO-SCLM

    Plasmid
    #216759
    Purpose
    AAV2 transfer vector for Cre recombinase-dependent expression of SuperClomeleon from the human elongation factor 1a promoter
    Depositor
    George Augustine
    Insert
    SuperClomeleon
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S30R and 11 AA deletion in the Cerulean CFP moiet…
    Promoter
    EF1a (human elongation factor 1 alpha)
    Available since
    April 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    216759-plasmid-map-sequence-id-429631

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  45. pCFP

    Plasmid
    #175593
    Purpose
    Lac inducible, AMP Resistant, cerulean gene on a low copy backbone. Used as a negative control to the pMesh1_CFP plasmid.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    CFP (cerulean)
    Use
    Tags
    Expression
    Mutation
    An improved monomeric CFP. See doi: 10.1038/nbt945
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175593-plasmid-map-sequence-id-348363

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  46. pMTB-Multibow-hC

    Plasmid
    #60993
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear cerulean
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    Cerulean
    Use
    Zebrafish
    Tags
    histone 2B tag
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  47. pMTB-Multibow-mC

    Plasmid
    #60986
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound cerulean
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    Cerulean
    Use
    Zebrafish
    Tags
    membrane tag
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    Jan. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  48. pAAV/CMV-SCLM

    Plasmid
    #216758
    Purpose
    AAV2 transfer vector with the CMV promoter for ubiquitous expression of SuperClomeleon
    Depositor
    George Augustine
    Insert
    SuperClomeleon followed by WPRE and human growth hormone polyA terminator
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S30R and 11 AA deletion in the Cerulean CFP moiet…
    Promoter
    CMV (cytomegalovirus)
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216758-plasmid-map-sequence-id-429489

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  49. Nemato

    Plasmid
    #24006
    Depositor
    Lawrence Cohen
    Insert
    VSFP
    Use
    Tags
    Cerulean and Citruline
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 2, 2010
    Availability
    Academic Institutions and Nonprofits only
    24006-plasmid-map-sequence-id-11277

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  50. SuperClomeleon

    Plasmid
    #203724
    Purpose
    An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride
    Depositor
    George Augustine
    Article
    Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309.
    Insert
    Cerulean linked topaz fluorescent protein
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Cerulean, modified Topaz Fluorescent Protein with…
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    203724-plasmid-map-sequence-id-403640

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Showing: 451 - 500 of 790 results