We narrowed to 13,473 results for: crispr cas9

1. pT3TS-nCas9n

   Plasmid

   #46757

   Depositor

   Wenbiao Chen

   Article

   Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T3

   Available Since

   Aug. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+hSpCas9

   Plasmid

   #51815

   Purpose

   Encode hSpCas9 for in vitro transcription with Sp6

   Depositor

   Masato Kinoshita

   Article

   Ansai et al Biol Open. 2014 Apr 11;3(5):362-71. doi:

   Insert

   humanized S.pyogenes Cas9
   

   Use

   CRISPR; Medaka, zebrafish

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available Since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICH41308::hCas9

   Plasmid

   #49770

   Purpose

   Level 0 hCas9 module

   Depositor

   Sophien Kamoun

   Article

   Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

   Insert

   hCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHO4d-Cas9

   Plasmid

   #67881

   Purpose

   Cas9nlsHis6 Bacterial Expression construct

   Depositor

   Michael Nonet

   Article

   Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and T7 epitope

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9_GFP

   Plasmid

   #44719

   Purpose

   Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Cas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAS9counter

   Plasmid

   #107192

   Purpose

   Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

   Depositor

   Steve Salipante

   Article

   Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

   Inserts

   Cas9
   sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   prab17

   Available Since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. SaCas9

   Plasmid

   #188494

   Purpose

   Expresses FLAG tagged SaCas9

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9

   Plasmid

   #188489

   Purpose

   Expresses FLAG tagged SpCas9

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAS9i

   Plasmid

   #141249

   Purpose

   Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

   Depositor

   Adam Bertl

   Article

   Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 (Cas9), pSNR52 (gRNA)

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAS9c

    Plasmid

    #141250

    Purpose

    Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 (Cas9), pSNR52 (gRNA)

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAS9cd

    Plasmid

    #141252

    Purpose

    Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 (Cas9), pSNR52 (gRNA)

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBbdCas9S_arr2

    Plasmid

    #149639

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbdCas9G_arr2

    Plasmid

    #149571

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbdCas9K_arr2

    Plasmid

    #149570

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBbdCas9H_arr2

    Plasmid

    #149573

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

    Plasmid

    #101217

    Purpose

    Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…

    Promoter

    T7

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9n(BB) (PX460)

    Plasmid

    #48873

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-hCas9

    Plasmid

    #51142

    Purpose

    Expresses hCas9 under the CAG promoter for CRISPR

    Depositor

    Izuho Hatada

    Article

    Horii et al Int J Mol Sci. 2013 Sep 30;14(10):19774-

    Insert

    hCas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pMA-SpCas9-g3

    Plasmid

    #80786

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 3/13/23 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMA-SpCas9-g4

    Plasmid

    #80787

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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