We narrowed to 14,168 results for: eng
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Plasmid#31482DepositorInsertTraffic Light Reporter 1.1 (Sce Target) EF Puro
UseLentiviralMutationmCherry has M9S and M16L mutations. This is what …Available sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330 p53
Plasmid#59910PurposepX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoterDepositorAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)
Plasmid#140003PurposeCMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
3xFLAG-dCas9/pCMV-7.1
Plasmid#47948PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationhuman codon-optimized, D10A + H840A (catalyticall…PromoterCMVAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hAAVS1 1L TALEN
Plasmid#35431PurposeTALEN designed to target the human AAVS1 locusDepositorInserthAAVS1 1L TALEN
ExpressionMammalianAvailable sinceMay 17, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hAAVS1 1R TALEN
Plasmid#35432PurposeTALEN designed to target the human AAVS1 locusDepositorInserthAAVS1 1R TALEN
ExpressionMammalianAvailable sinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti puro HA-Ubiquitin
Plasmid#74218PurposeLentiviral expression of HA tagged UbiquitinDepositorInsertubiquitin (UBB Human)
UseLentiviralTagsHAExpressionMammalianMutationSingle ubiquitin coding sequence (full length UBB…PromoterCMVAvailable sinceMarch 14, 2016AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
Lenti‐Cas9‐2A‐Blast
Plasmid#73310PurposeLentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoterDepositorInsertCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianPromoterEFS-NSAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST14-SpyCatcher
Plasmid#35044DepositorInsertSpyCatcher
TagsHis6ExpressionBacterialMutationDeletion of C-terminal till I113, and point mutat…Available sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV/D377Y-mPCSK9
Plasmid#58376PurposeExpresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosisDepositorHas serviceAAV8Insertproprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
UseAAVExpressionMammalianMutationD377YPromoterHCRApoE/hAATAvailable sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCasSA
Plasmid#98211PurposeCRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
sfGFP-N1
Plasmid#54737PurposeLocalization: N1 Cloning Vector, Excitation: 485, Emission: 507DepositorTypeEmpty backboneTagssfGFPExpressionMammalianAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pDIRECT_22C
Plasmid#91135PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRExpressionPlantAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330S-2
Plasmid#58778PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
sfGFP-pBAD
Plasmid#54519PurposeLocalization: Protein Expression Vector , Excitation: 485, Emission: 507DepositorTypeEmpty backboneTags6xHis and SfGFPExpressionBacterialAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pDEST-periHisMBP
Plasmid#11086DepositorTypeEmpty backboneTags6xHis, MBP, and MBP signal peptideExpressionBacterialPromoterTac (lactose/IPTG inducible)Available sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pACYC-GroEL/ES-TF
Plasmid#83923PurposeCo-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.DepositorInsertsGro EL/ES
Trigger Factor
ExpressionBacterialPromoterT7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV-EF1a-IRES-Neo
Plasmid#85139Purposelentiviral backboneDepositorTypeEmpty backboneUseLentiviralPromoterEF1aAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1_nMagHigh1-EGFP-CAAX
Plasmid#67300PurposeBlue light dependent photoswitching systemDepositorInsertnMagHigh1-EGFP-CAAX
ExpressionMammalianPromoterCMVAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDD104 (Peft-3::Cre)
Plasmid#47551PurposeCre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germlineDepositorInsertCre Recombinase
UseCre/LoxExpressionWormPromotereef-1A.1 (eft-3)Available sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by