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We narrowed to 13,201 results for: 109

Showing: 4951 - 5000 of 13201 results

  1. Desmoplakin II tension sensor (FL-based)

    Plasmid
    #118721
    Purpose
    The FL-based human desmoplakin II tension sensor detects forces in the range of 3-5 pN by changes in FRET between YPet(short) and mCherry.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II-[YPet(short)-FL-mCherry] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-FL-mCherry
    Expression
    Mammalian
    Mutation
    inserted FL-based tension sensor module after aa1…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118721-plasmid-map-sequence-id-230467

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  2. TFORF0584

    Plasmid
    #142651
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SRF (SRF Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    142651-plasmid-map-sequence-id-397744

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  19. Desmoplakin II no force control (FL-based)

    Plasmid
    #118724
    Purpose
    The no force control for the FL-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-FL-mCherry
    Expression
    Mammalian
    Mutation
    truncation after aa1353
    Promoter
    CMV
    Available since
    Dec. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    118724-plasmid-map-sequence-id-230471

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  24. (attB) tdMCP-tagged BRaf with MS2-circRNA

    Plasmid
    #226163
    Purpose
    Used to integrate 3xFlag-tdMCP-BRaf and MS2-circRNA barcodes into RNA-Protein Landing Pad cell lines
    Depositor
    Dustin Maly
    Article
    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.
    Inserts
    3xFlag-tdMCP-BRaf (BRAF Human)
    IRES-mCherry-P2A-PuroR
    MS2-circRNA barcode
    Use
    Bxb1 attb, no promoter
    Tags
    3xFlag-tdMCP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    226163-plasmid-map-sequence-id-483090

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  29. NanoLuc-NRAS (Q61R)-CRAF RBD-HaloTag BiBRET Vector

    Plasmid
    #236862
    Purpose
    Express NanoLuc(R)-NRAS (Q61R)-CRAF RBD-HaloTag(R) in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Inserts
    NRAS (NRAS Human)
    cRAF(RBD) (RAF1 Human)
    Use
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    Q61R
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236862-plasmid-map-sequence-id-471854

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  31. BiBRET Vector HaloTag-NRAS (Q61R)-BRAF RBD-NanoLuc

    Plasmid
    #236852
    Purpose
    Express HaloTag(R)-NRAS (Q61R)-BRAF RBD-NanoLuc(R) in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Inserts
    NRAS (NRAS Human)
    BRAF (RBD) (BRAF Human)
    Use
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    Q61R
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236852-plasmid-map-sequence-id-471850

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  33. TFORF1607

    Plasmid
    #144368
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    BAZ2B (BAZ2B Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    144368-plasmid-map-sequence-id-474400

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  34. MKC131_CCR5(SFFV-synEPOR-2A-YFP)

    Plasmid
    #232412
    Purpose
    AAV production plasmid for SFFV(synEPOR) vector from Figs. 2-3 that mediates HDR at CCR5 locus using CCR5 gRNA. YFP is followed by BGH polyA. AAV genome is flanked by AAV2 ITRs.
    Depositor
    Kyle Cromer
    Article
    Shah et al bioRxiv 2024.04.12.589260
    Insert
    Truncated Erythropoietin Receptor (EPOR Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    232412-plasmid-map-sequence-id-465038

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  35. MKC175_HBA1reg(synEPOR)

    Plasmid
    #232413
    Purpose
    AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBB gene includes introns; HBA1 UTRs flank H+C15BA1 cassette. HAs are ~400bp each.
    Depositor
    Kyle Cromer
    Article
    Shah et al bioRxiv 2024.04.12.589260
    Insert
    Truncated Erythropoietin Receptor (EPOR Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    232413-plasmid-map-sequence-id-466145

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  36. pLV-SFFV-CKAP4 C100A-WPRE-UbC-Emerald

    Plasmid
    #225949
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutation C100A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Evan Reid, Richard Eva
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    C100A
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225949-plasmid-map-sequence-id-446851

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  37. pLV-SFFV-CKAP4 CC-WPRE-UbC-Emerald

    Plasmid
    #225950
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutation double cysteine (CC) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Evan Reid, Richard Eva
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Additional cysteine inserted after Cys-100
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225950-plasmid-map-sequence-id-446852

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  38. pLV-SFFV-CKAP4(1-131)-WPRE-UbC-Emerald

    Plasmid
    #225955
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutant lacking the luminal domain under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Evan Reid, Richard Eva
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Truncated CKAP4 (1-131) lacking the luminal domain
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225955-plasmid-map-sequence-id-446861

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  42. HCA2-DuET

    Plasmid
    #213308
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HCA2 (HCAR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213308-plasmid-map-sequence-id-430846

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  50. TFORF2624

    Plasmid
    #144054
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CRTC3 (CRTC3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    144054-plasmid-map-sequence-id-400558

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Showing: 4951 - 5000 of 13201 results