We narrowed to 29,757 results for: REP

1. Cathepsin S

   Plasmid

   #84694

   Purpose

   for expression of Cathepsin S in Leishmania tarantolae

   Depositor

   Hans Brandstetter

   Article

   Freier et al Sci Rep. 2015 Aug 3;5:12707. doi: 10.103

   Insert

   cathepsin S (CTSS Human)
   

   Use

   Expression in leishmania tarantolae

   Tags

   his-tag

   Expression

   Mutation

   Promoter

   unknown

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-eDIO-EYFP

   Plasmid

   #104388

   Purpose

   PiggyBac plasmid encoding EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.

   Depositor

   Volker Busskamp

   Article

   Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10

   Insert

   Enhanced Yellow Fluorescent Protein
   

   Use

   Cre/Lox; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKanCMV-mRuby3-18aa-actin

   Plasmid

   #74255

   Purpose

   Mammalian expression of N-terminal fusion of mRuby3 to actin

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Insert

   actin
   

   Use

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and CMV

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. eSpCas9(1.1)_No_FLAG

   Plasmid

   #79877

   Purpose

   FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Untagged eSpCas9(1.1)

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. ECFP(W66A)-FRB-MoA

   Plasmid

   #67904

   Purpose

   Expresses a Mitochondria located FRB domain in mammalian cells (the ECFP is not fluorescent)

   Depositor

   Dorus Gadella

   Article

   van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

   Insert

   ECFP(W66A)-FRB-MoA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101103

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   VEGFR1-dCas9(C)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tol2-cmlc2-Trpa1b-2A-EGFP

   Plasmid

   #106405

   Purpose

   For expressing Trpa1b-2A-EGFP in zebrafish cardiomyocytes.

   Depositor

   Randall Peterson

   Article

   Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

   Inserts

   cmlc2 promoter
   Trpa1b (trpa1b Zebrafish)
   

   Use

   Gateway

   Tags

   2A-EGFP

   Expression

   Mutation

   Promoter

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-CT3G-CERP2

   Plasmid

   #175499

   Purpose

   All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-mCMV

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMK278 (mClover-Hygro)

   Plasmid

   #72794

   Purpose

   C-terminal tagging with mClover using CRIPSR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mClover-Hygro
   

   Use

   CRISPR

   Tags

   mClover

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-CAG-T-V5-GR-2A-H2BmCherry-IP

    Plasmid

    #171500

    Purpose

    Overexpression of T(Brachyury)-GR

    Depositor

    Hans Schöler

    Article

    Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

    Insert

    T
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKanCMV-mClover3-18aa-Tubulin

    Plasmid

    #74253

    Purpose

    Mammalian expression of N-terminal fusion of mClover3 to Tubulin

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Insert

    Tubulin
    

    Use

    Tags

    mClover3

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and CMV

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-UBC-MCP-mNeonGreen

    Plasmid

    #207581

    Purpose

    The lentiviral plasmids for expression of MCP-NeonGreen

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    MS2 coat protein mNeonGreen fusion
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    UBC

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMBA328

    Plasmid

    #214748

    Purpose

    Medium copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RBS(22821)-mcherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMBA333

    Plasmid

    #214745

    Purpose

    Medium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RiboJ-RBS(21992)-gfpmut3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACYCDuet_WTDHFR_WTTS

    Plasmid

    #91226

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Bmp6

    Plasmid

    #163590

    Purpose

    Expresses BMP-6 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp6 (Bmp6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. MDO1-c014

    Plasmid

    #173103

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    MACROD1 (MACROD1 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    S58-A325, codon optimized

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. LENG

    Plasmid

    #111162

    Purpose

    miR-E (miR-30 variant)-based RNAi with GFP reporter

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pirespuro2-CD86-mEos2

    Plasmid

    #98284

    Purpose

    mammalian expression of CD86-mEos2

    Depositor

    Mike Heilemann

    Article

    Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

    Insert

    CD86 (CD86 Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1a-loxP-GRAB_DA2m-loxP

    Plasmid

    #189751

    Purpose

    Excludes sensor expression in Cre-positive neurons

    Depositor

    Yulong Li

    Article

    Dai et al Cell Rep. 2022 Aug 23;40(8):111246. doi:

    Insert

    Dopamine sensor GRAB_DA2m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pENTR221-NFAT5

    Plasmid

    #79506

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NFAT5 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    NFAT5 (NFAT5 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pGEMT-PT2A-iRFP670-Tdtomato-GFP

    Plasmid

    #111817

    Purpose

    Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    iRFP670
    Tdtomato
    GFP
    

    Use

    Cloning intermediate

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. Gi-c001

    Plasmid

    #173078

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    GNAI1 (GNAI1 Human)
    

    Use

    Tags

    mCitrine

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. LOTUS-V/pcDNA3

    Plasmid

    #87127

    Purpose

    Bioluminescent and ratiometric voltage indicator

    Depositor

    Takeharu Nagai

    Article

    Inagaki et al Sci Rep. 2017 Feb 13;7:42398. doi: 10.10

    Insert

    LOTUS-V
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Lck-FRB-ECFP(W66A)

    Plasmid

    #67902

    Purpose

    Expresses a membrane located FRB domain in mammalian cells (the ECFP is not fluorescent)

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    Lck-FRB-ECFP(W66A)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FRB-ECFP(W66A)-Giantin

    Plasmid

    #67903

    Purpose

    Expresses a Golgi located FRB domain in mammalian cells (the ECFP is not fluorescent)

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    FRB-ECFP(W66A)-Giantin
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAG-Gdf5

    Plasmid

    #163596

    Purpose

    Expresses GDF-5 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Gdf5 (Gdf5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ARH2-c003

    Plasmid

    #173094

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    ADPRHL1 (ADPRHL1 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E2-K354, codon optimized

    Promoter

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TOPO Cre-2A-GFP

    Plasmid

    #68450

    Purpose

    TOPO construct expressing Cre-2A-GFP for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Cre-2A-GFP
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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30. pTRE:iRFP670-EFS:Cre-2A-GFP

    Plasmid

    #68477

    Purpose

    Inducible lentivirus expressing iRFP670, Cre, and GFP

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    iRFP670
    Cre-2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and pTRE

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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