We narrowed to 11,958 results for: pcDNA

1. pcDNA3.1/Puro-CAG-JEDI-1P

   Plasmid

   #202607

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-1P, expressed under strong mammalian promoter (CAG)

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   JEDI-1P
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-IDO1-p2a-eGFP

   Plasmid

   #187026

   Purpose

   Expresses IDO1 and GFP (seperately) in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Maassen et al Free Radic Biol Med. 2022 Aug 1;188:287-

   Insert

   Indoleamine 2,3-dioxygenase 1 (IDO1 Human)
   

   Use

   Tags

   P2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA 3.1 KBTBD4 R313PRR

   Plasmid

   #184629

   Purpose

   Mammalian expression vector with N-terminal Flag for KBTBD4 R313PRR expression in mammalian cells

   Depositor

   Vincenzo D'Angiolella

   Article

   Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

   Insert

   N-terminal 3xFlag tagged KBTBD4 R313PRR (KBTBD4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R313PRR

   Promoter

   CMV

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1+ SMN1 myc HIS

   Plasmid

   #71687

   Purpose

   Mammalian expression of SMN1

   Depositor

   Gideon Dreyfuss

   Insert

   SMN1 (SMN1 Human)
   

   Use

   Tags

   HIS and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)

   Plasmid

   #49077

   Purpose

   Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x HA and mCerulean

   Expression

   Mammalian

   Mutation

   262 silent mutations from native hNKCC1

   Promoter

   CMV

   Available since

   Nov. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO HIS HSPA8

   Plasmid

   #19541

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   heat shock 70kDa protein 8 (HSPA8 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1+ hnRNPK myc HIS

   Plasmid

   #71662

   Purpose

   Mammalian expression of hnRNPK

   Depositor

   Gideon Dreyfuss

   Insert

   hnRNPK (HNRNPK Human)
   

   Use

   Tags

   HIS and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA-NES-PhoCl2f-mCherry

   Plasmid

   #164048

   Purpose

   Optogenetic control of protein localization by PhoCl2f

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   NES-PhoCl2f-mCherry
   

   Use

   Tags

   kozak sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promotor

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-Flag-Tet1 CD Mut

   Plasmid

   #72218

   Purpose

   Express flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.

   Depositor

   Yi Zhang

   Article

   Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

   Insert

   Ten-Eleven Translocation 1 (Tet1 Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   The plasmid expresses TET1 catalytic domain (cata…

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-Frb-EGFP-HOTag6

    Plasmid

    #106919

    Purpose

    Express Frb-EGFP-HOTag6 fusion in mammalian cells.

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

    Insert

    Frb-EGFP-HOTag6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-EGFP-Cdc42-Q61L

    Plasmid

    #12986

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Cdc42 constitutively active (CDC42 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q61L Constitutively active

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA 3.1(-) mouse Egr2

    Plasmid

    #107997

    Purpose

    Expresses mouse Egr2 in mammalian cells

    Depositor

    Peter Johnson

    Article

    Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

    Insert

    Egr2 (Egr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.4 human alpha5 full

    Plasmid

    #221396

    Purpose

    Mammalian expression of human integrin alpha5 full length

    Depositor

    Timothy Springer

    Article

    Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

    Insert

    integrin alpha5 full length (ITGA5 Human)
    

    Use

    Tags

    CD33 secretion peptide (MPLLLLLPLLWAGALA) and St…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-TFEBS122D-MYC (#777)

    Plasmid

    #99956

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

    Insert

    TFEB (TFEB Human)
    

    Use

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    S122D

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-FLAG-Rheb-N153T

    Plasmid

    #19997

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    Ras homolog enriched in brain 1 - N153T (RHEB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed Asparagine 153 to Threonine

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1-FRB-Cre-HiBiT

    Plasmid

    #177912

    Purpose

    FRB-Cre-HiBiT tag

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

    Insert

    FRB-Cre-HiBiT
    

    Use

    Tags

    HiBiT tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5/FRT/TO GFP HSPH1

    Plasmid

    #19480

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPH1 (HSPH1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-Peredox-mCherry-NLS

    Plasmid

    #32384

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    Peredox-mCherry-NLS
    

    Use

    Tags

    Nuclear Localization Seq

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-PB2-B/BRISBANE/60/2008

    Plasmid

    #187917

    Purpose

    Expresses PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase basic protein 2
    

    Use

    Tags

    UNTAGGED

    Expression

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5/FRT/TO_Beta4Gal-HaloTag7

    Plasmid

    #175536

    Purpose

    Expression of HaloTag7 at the at the Gogli apparatus of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Beta4Gal-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HA GSK3 beta K85A pcDNA3

    Plasmid

    #14755

    Depositor

    Jim Woodgett

    Article

    Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

    Insert

    GSK3 beta K85A (GSK3B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K85A. Kinase dead mutant.

    Promoter

    Available since

    April 12, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pcDNA-miR-21 toehold switch

    Plasmid

    #184756

    Purpose

    Expresses miR-21 sensor with RFP output

    Depositor

    Allen Liu

    Article

    Wang et al ACS Synth Biol. 2019 May 17;8(5):1079-10

    Insert

    Red Fluorescence Protein
    

    Use

    Tags

    miR-21 toehold switch

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-NGFP-VMAn-FKBP

    Plasmid

    #70225

    Purpose

    Expresses N-terminal portion of GFP along with VMA intein for protein splicing

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    eGFP
    

    Use

    Tags

    FK506- and rapamycin-binding protein (FKBP), Link…

    Expression

    Mammalian

    Mutation

    Changed Glu 125 to Ile, Deleted amino acids 129-2…

    Promoter

    CMV

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pcDNA3-FRB-VMAc-CGFP

    Plasmid

    #70226

    Purpose

    Expresses C-terminal portion of GFP along with VMA intein for protein splicing

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    eGFP
    

    Use

    Tags

    FKBP-rapamycin binding (FRB) domain of the mammal…

    Expression

    Mammalian

    Mutation

    Changed Ile 129 to Cys, Deleted amino acids 1-128

    Promoter

    CMV

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pcDNA3-6XHis-human SUMO 1

    Plasmid

    #133770

    Purpose

    express 6Xhis- SUMO 1 protein

    Depositor

    Martine Roussel

    Article

    Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

    Insert

    SUMO 1 (SUMO1 Human)
    

    Use

    Tags

    6XHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNAI-GAL4-CREB-S133A

    Plasmid

    #46770

    Purpose

    Expression of GAL4 fusion to CREB-S133A

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    GAL4(1-147)-CREB-S133A (Creb1, GAL4 Rat, Budding Yeast)
    

    Use

    Tags

    GAL4

    Expression

    Mammalian

    Mutation

    Changed CREB serine 133 to Alanine

    Promoter

    CMV

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNAI-GAL4-CREB-S142A

    Plasmid

    #46771

    Purpose

    Expression of GAL4 fusion to CREB-S142A

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    GAL4(1-147)-CREB-S142A (Creb1, GAL4 Rat, Budding Yeast)
    

    Use

    Tags

    GAL4

    Expression

    Mammalian

    Mutation

    Changed CREB serine 142 to Alanine

    Promoter

    CMV

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA5/FRT/TO HIS HSPA1A

    Plasmid

    #19537

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 1A (HSPA1A Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 1236 pcDNA3 Myr HA Akt3

    Plasmid

    #9017

    Depositor

    William Sellers

    Insert

    Akt3 (AKT3 Human)
    

    Use

    Tags

    HA and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDNA-myc-HisA-Sirt3

    Plasmid

    #24924

    Depositor

    Toren Finkel

    Article

    Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 1

    Insert

    Sirt3 (SIRT3 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

31. pcDNA3.1/Puro-CAG-ASAP1

    Plasmid

    #52519

    Purpose

    For mammalian expression of the fluorescent voltage sensor ASAP1

    Depositor

    Michael Lin

    Article

    St-Pierre et al Nat Neurosci. 2014 Apr 22. doi: 10.1038/

    Insert

    Accelerated Sensor of Action Potentials 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. HA-BirA*-pDEST-N-pcDNA5/FRT/TO

    Plasmid

    #118375

    Purpose

    Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    HA-BirA*

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-myc-his-BACH1 WT

    Plasmid

    #17642

    Depositor

    Ronny Drapkin

    Article

    Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

    Insert

    BACH1 (BRIP1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3-Mito-ExRai-AMPKAR

    Plasmid

    #192448

    Purpose

    ExRai-AMPKAR targeted to the mitochondrial outer membrane.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    Mito-ExRai-AMPKAR
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3-AU1-mTOR-S2215Y

    Plasmid

    #26037

    Depositor

    Fuyuhiko Tamanoi

    Article

    Sato et al Oncogene. 2010 May 6. 29(18):2746-52.

    Insert

    mammalian target of rapamycin-S2215Y (Mtor Rat)
    

    Use

    Tags

    AU1

    Expression

    Mammalian

    Mutation

    S2215Y

    Promoter

    Available since

    Sept. 21, 2010

    Availability

    Academic Institutions and Nonprofits only

36. pcDNA4/TO/Strep-HA-ZAK alpha

    Plasmid

    #141193

    Purpose

    Expresses Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 (MAP3K20 Human)
    

    Use

    Tags

    Strep, HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA-HDAC6.ΔBuz-FLAG

    Plasmid

    #30484

    Depositor

    Tso-Pang Yao

    Article

    Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

    Insert

    HDAC6 (HDAC6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of the C-terminal Ubiquitin binding doma…

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA5/FRT/TO-GRB2-GFP

    Plasmid

    #86873

    Purpose

    To express GRB2 with a GFP tag at C terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    GRB2 (GRB2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-FLAG-MTOR-L1460P

    Plasmid

    #69006

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-L1460P (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Leucine 1460 to Proline

    Promoter

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3-FKBP-EGFP-HOTag3

    Plasmid

    #106924

    Purpose

    Expresses FKBP-EGFP-HOTag3 in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

    Insert

    FKBP-EGFP-HOTag3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge