We narrowed to 15,732 results for: LEA

1. SP_C3

   Plasmid

   #228159

   Purpose

   Plasmid contains the secretion signal peptide sequence of the yeast (strain ATCC 204508 / S288c) PIR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

   Depositor

   Joseph Brock

   Article

   Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

   Insert

   sp_Q03178_PIR1_YEAST:0-18
   

   Use

   Synthetic Biology

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNK6031

   Plasmid

   #219739

   Purpose

   MoClo-compatible Level 0 - promoter + 5U vector carrying promoter CmYLCV

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

   Insert

   Cestrum yellow leaf curling virus (CmYLCV) promoter
   

   Use

   Synthetic Biology

   Expression

   Plant

   Promoter

   CmYLCV

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDestTol2pA ins:TDimer mVenus

   Plasmid

   #210520

   Purpose

   Expresses a tandem dimer of mVenus in zebrafish pancreatic beta cells using a zebrafish insulin promoter

   Depositor

   Jonathan Rocheleau

   Article

   Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

   Insert

   Tandem dimer mVenus
   

   Use

   Zebrafish expression, tol2 kit gateway-compatible

   Promoter

   Zebrafish insulin promoter

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDestTol2pA ins:R198P mVenus

   Plasmid

   #210519

   Purpose

   Expresses mVenus-tagged R198P mutant of Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoter

   Depositor

   Jonathan Rocheleau

   Article

   Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

   Insert

   Apollo-NADP+
   

   Use

   Zebrafish expression, tol2 kit gateway-compatible

   Tags

   mVenus

   Mutation

   R198P

   Promoter

   Zebrafish insulin promoter

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDestTol2pA ins:Apollo-NADP+ mVenus

   Plasmid

   #210518

   Purpose

   Expresses mVenus-tagged Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoter

   Depositor

   Jonathan Rocheleau

   Article

   Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

   Insert

   Apollo-NADP+
   

   Use

   Zebrafish expression, tol2 kit gateway-compatible

   Tags

   mVenus

   Promoter

   Zebrafish insulin promoter

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p35S::SP-mCherry-GFP-HDEL

   Plasmid

   #159097

   Purpose

   Positive control for FRET in plant ER / nuclear envelope.

   Depositor

   Joe McKenna

   Article

   McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

   Insert

   SP-mCherry-GFP-HDEL
   

   Tags

   mCherry, GFP

   Expression

   Plant

   Promoter

   35S

   Available Since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PX458_3XHA_dFnCas9_KRAB_T2A_eGFP

   Plasmid

   #200389

   Purpose

   3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

   Depositor

   Debojyoti Chakraborty

   Insert

   PX458_3XHA_dFnCas9_KRAB_eGFP
   

   Use

   CRISPR

   Tags

   3xHA-NLS and SV40NLS-KRAB-T2A-EGFP

   Expression

   Mammalian

   Promoter

   CBH

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET15b I-SceI (Ctermhis) K223I

   Plasmid

   #195615

   Purpose

   Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activity

   Depositor

   Frederick Gimble

   Article

   Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

   Insert

   I-SceI
   

   Expression

   Bacterial

   Mutation

   C2694G, G2345C, G2351C, A3365T, A3366T

   Promoter

   T7

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET15b I-SceI (Ctermhis) K122I

   Plasmid

   #195614

   Purpose

   Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activity

   Depositor

   Frederick Gimble

   Article

   Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

   Insert

   I-SceI
   

   Expression

   Bacterial

   Mutation

   C2458G, A2826T, A2827T

   Promoter

   T7

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET PI-SceI C-His K301A

    Plasmid

    #195611

    Purpose

    Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K301A active site mutation

    Depositor

    Frederick Gimble

    Article

    He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

    Insert

    PI-SceI
    

    Expression

    Bacterial

    Mutation

    A822G, A823C, A824T

    Promoter

    T7

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET PI-SceI C-His

    Plasmid

    #195610

    Purpose

    Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter with a carboxyl-terminal histidine tag

    Depositor

    Frederick Gimble

    Article

    He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

    Insert

    PI-SceI
    

    Expression

    Bacterial

    Mutation

    T825C, C900G, A901C, A903C, T1131G, T1137C, T1227…

    Promoter

    T7

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pENTR-ZmIAA29 (yeast codon optimized)

    Plasmid

    #188441

    Purpose

    Zea Mays Nuclear Auxin Signaling Repressor IAA29 with yeast codon optimization

    Depositor

    Britney Moss

    Article

    Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

    Insert

    Auxin Signaling Repressor IAA29 (yeast codon optimized)
    

    Use

    Cloning

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGI3EM31

    Plasmid

    #135491

    Purpose

    Agrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    hph SpunH2A/Bpr hph-mCerulean3
    

    Tags

    mCerulean3

    Expression

    Bacterial and Yeast

    Promoter

    SpunH2A/Bpr (divergent)

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMM0649

    Plasmid

    #129010

    Purpose

    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporter

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    

    Expression

    Yeast

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMM0655

    Plasmid

    #129016

    Purpose

    Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporter

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    

    Expression

    Yeast

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMM0654

    Plasmid

    #129015

    Purpose

    Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporter

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    

    Expression

    Yeast

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMM0653

    Plasmid

    #129014

    Purpose

    Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporter

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    

    Expression

    Yeast

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMM0650

    Plasmid

    #129011

    Purpose

    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporter

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    

    Expression

    Yeast

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMM0648

    Plasmid

    #129009

    Purpose

    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effects

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3 homology-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-homology KanR-ColE1
    

    Expression

    Yeast

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMM0651

    Plasmid

    #129012

    Purpose

    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporter

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    

    Expression

    Yeast

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge