We narrowed to 22,012 results for: his

1. pET-Duet1-6xHis-TEV-LC3BGlydelta5C

   Plasmid

   #190237

   Purpose

   Plasmid for the expression and purification of 6xHis-TEV-LC3BGlydelta5C. Internal reference: SMC893.

   Depositor

   Sascha Martens

   Article

   Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Insert

   MAP1LC3B (MAP1LC3B Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

   Plasmid

   #124660

   Purpose

   reporter protein which emits bright blue fluorescence

   Depositor

   Yutaka Kuroda

   Article

   Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

   Insert

   Gaussia luciferase-C9D-TGinsert
   

   Use

   Tags

   IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

   Expression

   Bacterial

   Mutation

   changed glutamic acid 100 to alanine, and glycine…

   Promoter

   T7 promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDYU1G/mRuby3–histone H1; γ-tubulin-mEGFP

   Plasmid

   #154303

   Purpose

   Expresses mRuby3–histone H1 and γ-tubulin-mEGFP from actin15 promoter in Dictyostelium discoideum

   Depositor

   Shigehiko Yumura

   Article

   Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

   Inserts

   histone H1
   γ-tubulin
   

   Use

   Dictyostelium expression

   Tags

   mEGFP and mRuby3

   Expression

   Mutation

   Promoter

   actin15

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1/Myc-His(-)-HSulf-2 deltaCC

   Plasmid

   #13006

   Depositor

   Steven Rosen

   Article

   Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

   Insert

   human sulfatase 2 deltaCC (SULF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Cysteine 88 to Alanine

   Promoter

   Available since

   Oct. 30, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.Flag.HERC2-HECT-C4762A.6xHis

   Plasmid

   #39228

   Depositor

   Aziz Sancar

   Article

   Kang et al Nucleic Acids Res. 2011 Apr;39(8):3176-8

   Insert

   HERC2 HECT Domain (HERC2 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   C4762A

   Promoter

   T7

   Available since

   July 31, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pET28a-ybbr-HIS-ddFLN4-K10

   Plasmid

   #101718

   Purpose

   K10 peptide with ddFLN4 fingerprint domain and N-terminal ybbr tag for covalent immobilization - resulting in high forces

   Depositor

   Hermann Gaub

   Article

   Milles et al Science. 2018 Mar 30;359(6383):1527-1533

   Insert

   ddFLN4 fingerprint, K10 peptide (KRT10 Human, Dictyostelium discoideum)
   

   Use

   Tags

   HIS and ybbr

   Expression

   Bacterial

   Mutation

   C18S in ddFLN4

   Promoter

   T7

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. HOXA2 (murine) HIS-tag pET

   Plasmid

   #8557

   Depositor

   Corey Largman

   Insert

   HOXA2 (Hoxa2 Mouse)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   contains 5 additional amino acids from 5' un…

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pBIG1b_nsp7-His6-nsp8 (SARS-CoV-2)

   Plasmid

   #169180

   Purpose

   Baculoviral transfer vector to express nsp7-His6-nsp8 fusion protein (SARS-CoV-2) in insect cells

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   nsp7-His6-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   His6 (internal tag linking nsp7 and nsp8): nsp7-H…

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSumo-His6- Sumo-AtTPL(1-209)

   Plasmid

   #177856

   Purpose

   Bacterial Expression of TPL

   Depositor

   Karsten Melcher

   Article

   Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

   Insert

   TPL (TPL Mustard Weed)
   

   Use

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMG058 MBP-GS 634-666-His

    Plasmid

    #154077

    Purpose

    Expresses MBP-GS 634-666-His (human 33aa Glycogen Synthase peptide as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    GYS1 (GYS1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET23A-Fucosidase-E1_10125-Ruminococcus_gnavus-His

    Plasmid

    #207665

    Purpose

    Expresses fucosidase E1_10125 from Ruminococcus gnavus in BL21 cells, purified using His-tag

    Depositor

    Robert de Vries

    Article

    Spruit et al bioRxiv 2023.12.15.571923

    Insert

    Fucosidase E1_10125
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    amino acids 34-584 were used

    Promoter

    T7 promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HOXA11 (murine) HIS-tag pET

    Plasmid

    #8517

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HOXA11 (Hoxa11 Mouse)
    

    Use

    Tags

    T7

    Expression

    Bacterial

    Mutation

    makes protein by Western

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pRSETB-S28 histone 3 phosphorylation reporter

    Plasmid

    #32788

    Depositor

    Alice Ting

    Article

    Lin et al Angew Chem Int Ed Engl. 2004 May 24;43(2

    Insert

    CFP-14-3-3tau-H3 peptide-YFP
    

    Use

    Tags

    CFP and YFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pNUT N6His K206E/K534A hTFNG

    Plasmid

    #70133

    Purpose

    Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe or the C-lobe

    Depositor

    Anne Mason, Ross MacGillivray

    Article

    Halbrooks et al Biochemistry. 2003 Apr 8;42(13):3701-7.

    Insert

    N6His K206e/K534A hTFNG (TF Human)
    

    Use

    Tags

    N-terminal signal peptide, 4 aa link, 6 His, Fact…

    Expression

    Mammalian

    Mutation

    changed Lys206 to a glutamic acid and Lys534 to a…

    Promoter

    SV40

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA6a-link-yoPA-mCherry-SpHis5

    Plasmid

    #44855

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized photoactivatable mCherry with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoPA-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. HOXB2 (human) HIS-tag pET

    Plasmid

    #8522

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB2 (HOXB2 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Starts at AA #2; makes protein by Western

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA4-RI1-332-MYC-His6

    Plasmid

    #185351

    Purpose

    Mammalian expression of Ribophorin I

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    Ribophorin I (RPN1 Human)
    

    Use

    Tags

    Myc, 6xHis

    Expression

    Mammalian

    Mutation

    aa 1-332 only

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-28a(+)-His(6)-TEV-AtPCO4-1(wt)

    Plasmid

    #191801

    Purpose

    Full length wt AtPCO4 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO4-1
    

    Use

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. P47-COMP-blac-flag-his

    Plasmid

    #111000

    Purpose

    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

    Depositor

    Gavin Wright

    Article

    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

    Insert

    secreted ookinete protein, putative (PSOP12) (PF3D7_0513700 Synthetic)
    

    Use

    Tags

    Exogenous signal peptide of mouse origin and rat …

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SOAP-COMP-blac-flag-his

    Plasmid

    #110997

    Purpose

    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

    Depositor

    Gavin Wright

    Article

    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

    Insert

    secreted ookinete adhesive protein (SOAP) (PF3D7_1404300 Synthetic)
    

    Use

    Tags

    Exogenous signal peptide of mouse origin and rat …

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CTRP-COMP-blac-flag-his

    Plasmid

    #110994

    Purpose

    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

    Depositor

    Gavin Wright

    Article

    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

    Insert

    circumsporozoite- and TRAP-related protein (CTRP) (CTRP Synthetic)
    

    Use

    Tags

    Exogenous signal peptide of mouse origin and rat …

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. P25-COMP-blac-flag-his

    Plasmid

    #110991

    Purpose

    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

    Depositor

    Gavin Wright

    Article

    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

    Insert

    ookinete surface protein P25 (PF10_0303 Synthetic)
    

    Use

    Tags

    Exogenous signal peptide of mouse origin and rat …

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. WARP-COMP-blac-flag-his

    Plasmid

    #110989

    Purpose

    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

    Depositor

    Gavin Wright

    Article

    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

    Insert

    von Willebrand factor A-domain related protein (WARP) (PF08_0136b Synthetic)
    

    Use

    Tags

    Exogenous signal peptide of mouse origin and rat …

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pB-6HIS-TEV-mEGFP,IRES-NGFR

    Plasmid

    #164521

    Purpose

    All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal His-TEV-GFP tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

    Depositor

    Mitchell Guttman

    Article

    Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMIR-Dsred-IRES-His Keap

    Plasmid

    #62457

    Purpose

    expresses his tagged human Keap1 protein

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    HisTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-dSpCas9-HF1-VP64-6xHis

    Plasmid

    #92118

    Purpose

    Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, N497A, R661A, Q695A, H840A, Q926A

    Promoter

    T7

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET11-His6-Pif6-sfGFP-ssrA

    Plasmid

    #140891

    Purpose

    Expression of PIF-sfGFP-ssrA in E. coli

    Depositor

    Neal Devaraj

    Article

    Bhattacharya et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

    Insert

    PIF-sfGFP-ssrA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCI-neo-His-MS2BP-G3BP1-WT

    Plasmid

    #136008

    Purpose

    WT G3BP1 inserted with MS2BP tagged on the N-terminus to use with the Tethering Assay (MS2)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Tags

    MS2BP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p6042 pcDNA-Xpress-His-USP5

    Plasmid

    #24744

    Depositor

    Peter Howley

    Article

    Vos et al J Virol. 2009 Sep . 83(17):8885-92.

    Insert

    USP5 (USP5 Human)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2010

    Availability

    Academic Institutions and Nonprofits only

30. pcDNA4-His-Max-A-PQBP1-Y65C

    Plasmid

    #25803

    Depositor

    Marius Sudol

    Article

    Tapia et al J Biol Chem. 2010 Jun 18;285(25):19391-4

    Insert

    PQBP1 with Y65C mutation (PQBP1 Human)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Tyrosine 65 within the WW domain of PQBP1 is chan…

    Promoter

    Available since

    July 21, 2010

    Availability

    Academic Institutions and Nonprofits only