163,606 results

1. pMPRAdonor2

   Plasmid

   #49353

   Purpose

   minP-luc2 donor plasmid for MPRA library construction

   Depositor

   Tarjei Mikkelsen

   Article

   Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

   Insert

   luc2
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   minP

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNUT N6His hTFNG

   Plasmid

   #67240

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin in mammalian cells

   Depositor

   Anne Mason, Ross MacGillivray

   Article

   Mason et al Biochemistry. 2002 Jul 30;41(30):9448-54

   Insert

   human serum transferrin (TF Human)
   

   Use

   Tags

   N-terminal signal peptide, 4 aa link, 6 His, Fact…

   Expression

   Mammalian

   Mutation

   Asn413 Asp, Asn611Asp

   Promoter

   SV40

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-EFS-LSL-nlsGFP-shRNA

   Plasmid

   #175584

   Purpose

   Conditional express shRNA with control of LoxP

   Depositor

   Christian Wolfrum

   Article

   Sun et al Nature. 2020 Nov;587(7832):98-102. doi:

   Type

   Empty backbone

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG241 FKBP-CFAST10

   Plasmid

   #130814

   Purpose

   Expresses FKBP-CFAST10 in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

   Insert

   FKBP-CFAST10
   

   Use

   Tags

   Myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pN1-RnKIF5C(1-560,Δ6)-Halo-Flag

   Plasmid

   #188072

   Purpose

   Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags. The delta6 mutation removes the first 6 amino acids.

   Depositor

   Kristen Verhey

   Article

   Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e

   Insert

   rat KIF5C (Kif5c Rat)
   

   Use

   Tags

   Flag and Halo

   Expression

   Mammalian

   Mutation

   aa1-560, deletion of aa2-6

   Promoter

   CMV

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL3402 (pTnsABf_Pse)

   Plasmid

   #200901

   Purpose

   Expresses human codon-optimized Pseudoalteromonas TnsA-TnsB fusion from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   PseTnsA-TnsB
   

   Use

   Tags

   Internal NLS between TnsA-TnsB fusion

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. RRL.sin.cPPT.SFFV/Flag-Firefly luciferase.IRES-puro.WPRE (MT05)

   Plasmid

   #139446

   Purpose

   Lentiviral vector genome to ectopically express Firefly luciferase

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   FLAG-tag Firefly luciferase
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-BG4-NLS-mCer

   Plasmid

   #211448

   Purpose

   mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence

   Depositor

   Helmut Pospiech

   Insert

   Anti-DNA G-quadruplex single chain antibody BG4
   

   Use

   Tags

   NLS-mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSFFV_sfCherry3C(1-10)

   Plasmid

   #117482

   Purpose

   Lentiviral vector for constitutive expression of sfCherry3C(1-10)

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Insert

   sfCherry3C(1-10)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   5 substitutions (K45R, G52D, T106A, K182R, N194D)…

   Promoter

   SFFV

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOPINE GFP nanobody

    Plasmid

    #49172

    Purpose

    Bacterial expression of GFP nano body

    Depositor

    Brett Collins

    Article

    Kubala et al Protein Sci. 2010 Dec;19(12):2389-401. d

    Insert

    GFP nanobody
    

    Use

    Tags

    HHHHHH

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAcBac2.tR4-OMeYRS/GFP*

    Plasmid

    #50831

    Purpose

    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells

    Depositor

    Peter Schultz

    Article

    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

    Inserts

    two-copy Tyr tRNA cassette
    EGFP*
    OMeYRS
    two copy Tyr tRNA cassette
    

    Use

    Baculoviral

    Tags

    His, Myc-6xHis, and WPRE

    Expression

    Insect

    Mutation

    Y39TAG and able to charge various unnatural amino…

    Promoter

    CAG, CMV, and U6 and H1

    Available Since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSL1749 (pDonor, R*MmeI)

    Plasmid

    #160733

    Purpose

    Mini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST donor DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSL5069 (pDonor(AAVS1),CRISPR_Pse)

    Plasmid

    #200904

    Purpose

    Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    Mini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28:GFP

    Plasmid

    #60733

    Purpose

    pET28 driving expression of GFP, biobrick part Bba_E0040

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6a-6xGLY-3xFLAG-kanMX6

    Plasmid

    #20754

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-VIVIT

    Plasmid

    #11106

    Depositor

    Anjana Rao

    Article

    Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

    Insert

    VIVIT
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBHSTC104

    Plasmid

    #124356

    Purpose

    Plasmid expressing N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin

    Depositor

    Hideo Iwai

    Article

    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

    Insert

    gp41-1 N-intein and C-intein (bicistronic)
    

    Use

    Tags

    CcdA-C (SFAD)

    Expression

    Bacterial

    Mutation

    Promoter

    STC

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Tol2-BetaActin-SecA5-YFP

    Plasmid

    #105664

    Purpose

    Ubiquitously expresses a secreted and fluorescently tagged Annexin5 for in-vivo apoptosis reporter

    Depositor

    Qing Deng

    Article

    Hsu et al Front Immunol. 2018 Mar 29;9:607. doi: 1

    Insert

    Secreted Annexin5-EYFP (ANXA5 Human)
    

    Use

    Tol2-gateway

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Beta-Actin

    Available Since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRRLsinPGK_IRFP720_WPRE

    Plasmid

    #91785

    Purpose

    Constitutive expression of IRFP720 using PGK promoter in a 3rd generation lentiviral backbone.

    Depositor

    Beverly Torok-Storb

    Insert

    iRFP720
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. His6-TEV-CRBN-midi

    Plasmid

    #215330

    Purpose

    CRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader design

    Depositor

    Alessio Ciulli

    Article

    Kroupova et al Nat Commun. 2024 Oct 15;15(1):8885. doi:

    Insert

    CRBN (CRBN Human)
    

    Use

    Tags

    6xHis and TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 41-187 followed by a Gly-Ser…

    Promoter

    lacI

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA3/Myc-DNMT1

    Plasmid

    #36939

    Purpose

    Mammalian expression of human DNMT1 with myc tag

    Depositor

    Arthur Riggs

    Article

    Li et al J Biol Chem. 2006 Jul 14;281(28):19489-5

    Insert

    DNMT1 (DNMT1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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22. Rtn4b-GFP

    Plasmid

    #186952

    Purpose

    Expresses Rtn4b connected with C-terminus AcGFP1

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    RTN4B (RTN4 Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. NFAT luciferase reporter

    Plasmid

    #10959

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

    Insert

    IL-2 promoter (IL2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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24. MECP2 in pENTR

    Plasmid

    #216592

    Purpose

    gateway cloning donor vector containing MECP2

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pHIV-EGFP.Flag-EZH2

    Plasmid

    #220243

    Purpose

    Expresses wild-type EZH2 for knockout/rescue experiments in mammalian cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pORANGE Cloning template vector

    Plasmid

    #131471

    Purpose

    Cloning template form ORANGE method based knock-in constructs

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. pDV06.2c_DART-VADAR_iRFP720-sensor

    Plasmid

    #200410

    Purpose

    pDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Closed-loop DART VADAR sensor detecting iRFP720
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. eSpCas9(1.1)

    Plasmid

    #71814

    Purpose

    Expresses high specificity SpCas9. Px330-like plasmid.

    Depositor

    Feng Zhang

    Article

    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

    Has Service

    Cloning Grade DNA

    Insert

    enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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29. pdCas9-J23111

    Plasmid

    #113149

    Purpose

    Plasmid constitutively expressing dCas9 protein

    Depositor

    Chong Zhang

    Article

    Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A & H840A

    Promoter

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. phDLX2-N174

    Plasmid

    #60860

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses DLX2 under the EF1a promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    DLX2 (DLX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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