167,150 results
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Plasmid#171428PurposeExpression of FKBP-(GGS)X6-HaloTag C58 in mammalian cellsDepositorInsertFKBP-(GGS)X6-HaloTag C58
ExpressionMammalianAvailable SinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2/HB
Plasmid#26557DepositorInsertpT2/HB
ExpressionBacterialAvailable SinceDec. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET29a-human histone 4-in-One
Plasmid#223237PurposePolycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4DepositorInsertsHis6-VSVG-Histone H2A
Histone H2B
Histone H3
Histone H4-TEV-His6-Dual StrepII
TagsDual StrepII, His6, TEV protease site, and VSVGExpressionBacterialMutationA103GPromoterT7Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-DIO-NaChBac-dTomato
Plasmid#236252PurposeCre dependent NaChBac-TdTomato expressionDepositorInsertNaChBac-TdTomato
UseAAVTagsTdTomatoExpressionMammalianPromoterhSynAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG141
Plasmid#69759PurposeRetroviral expression of CENP-BDepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpRY-ABE8e
Plasmid#185671PurposeExpresses SpRY-ABE8e in mammalian cellsDepositorInsertecTadA(8e)-nSpRY
UseCRISPRExpressionMammalianMutationSpRY D10AAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-CD33-Fc(DAPA)-AviTag-6xHis
Plasmid#156694PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertCD33 (CD33 Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC1-HyPerRed-mito
Plasmid#60247PurposeMitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.DepositorInsertHyPerRed-mito
TagsMitochondrial targeting signalExpressionMammalianPromoterCMVAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 CDK7 HA (P#633)
Plasmid#14647DepositorAvailable SinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUCC001
Plasmid#124451PurposeMulti-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targetsDepositorInsertHH-BsaI
ExpressionYeastMutationgRNA expression cassette from pUDP002 modified to…PromoterTDH3 promoter from S.cerevisiae (already present …Available SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYC1446
Plasmid#158720PurposeIntegrating plasmid expressing Sth1 Cas9 and the cognate sgRNADepositorInsertSth1 sgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLU-CMV-Flag-hATG5 WT
Plasmid#111803PurposeMammalian Expression of hATG5DepositorAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC013 - Twinstrep-SUMO-huLwCas13a
Plasmid#90097PurposeTwinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.DepositorInsertLwCas13a
UseCRISPRTags6xHis-Twin Strep-SUMOExpressionBacterialAvailable SinceJune 13, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTol2-10xUAS-Voltron-ST
Plasmid#119040PurposeUAS-driven expresion of soma-localized Voltron in zebrafishDepositorInsertVoltron-ST
UseZebrafish expressionPromoterE1bAvailable SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF3180
Plasmid#144656PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBECKP-km
Plasmid#117235PurposeA cytidine deaminase-mediated base-editing plasmid in Klebsiella PneumoniaeDepositorInsertncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHnCBS1D
Plasmid#52065Purposeexpresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducibleDepositorInsertCbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D
ExpressionBacterialAvailable SinceApril 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF3274
Plasmid#144750PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNK2657
Plasmid#219741PurposeMoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiensDepositorInserthispidin- synthase from Mycena citricolor
UseSynthetic BiologyAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-jGCaMP8m
Plasmid#172388PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertGCaMP8m
UseNeurotropic virusAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE TRE dCas9-KRAB
Plasmid#50917PurposeTet-regulatable dCas9-KRAB 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and KRABExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterTREAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJ404-DogCatcher-sfGFP
Plasmid#171930PurposeExpress DogCatcher-superfolder GFP protein in bacterial cytoplasmDepositorInsertDogCatcher-sfGFP
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT5Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
145-pGL3ctrl-3' UTR
Plasmid#28175DepositorInsertLuciferase flanked by tp53 UTRs (TP53 Human)
UseLuciferaseExpressionMammalianMutationNote that insert length is based on sum of the 2 …Available SinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pR26 CAG AsiSI/MluI
Plasmid#74286PurposeGene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or MluiDepositorInsertRosa26 5-homology region
Available SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAB16 (pmyo-3::QuasAr)
Plasmid#130272PurposeExpresses the voltage sensor QuasAr in BWMs of C. elegans.DepositorInsertQuasAr
ExpressionWormAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(0)
Plasmid#199166Purposeused to express a neutral GFP variant in E. coliDepositorInsertGFP(0)
TagsMGHHHHHHGGAMutationE16R, I138R, D207R, N222K, L246RPromoterT7Available SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-HaloTag N58-(GGS)X6-FRB
Plasmid#171427PurposeExpression of HaloTag N58-(GGS)X6-FRB in mammalian cellsDepositorInsertHaloTag N58-(GGS)X6-FRB
ExpressionMammalianAvailable SinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pScaf-5544.4
Plasmid#111524PurposepScaf phagemid with insert for generating DNA origami scaffold 5544 bases in length (v4)DepositorInsertpScaf-KH'
ExpressionBacterialAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag_HsIRE1a_deltaP29_D408_pBabePuro
Plasmid#58422Purposeretroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408DepositorInsertIRE1a (ERN1 Human)
UseRetroviralTagsFLAG and preprotrypsin signal sequenceExpressionMammalianMutationDeleted amino acids P29 to D408Available SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJH127
Plasmid#162483PurposeSEC plasmid containing LG1 homology arms and myo-2p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.DepositorInsertmyo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
UseCRISPRTagsmyo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTRExpressionWormAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only