169,365 results

Showing: 2101 - 2120 of 169365 results

Results per page:

Sort results by:

pHEFI-OptoCARCD19-mScarlet

Plasmid

#170465

Purpose

Expresses an optically-controllable Chimeric antigen receptor that binds CD19 antigen, fused to mScarlet fluorophore

Depositor

John James

Article

Harris et al Mol Syst Biol. 2021 May;17(5):e10091. do

Insert

OptoCAR-CD19

Use

Lentiviral

Tags

mScarlet

Expression

Mammalian

Promoter

EF1alpha

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX304 CMV HA-HaloTag-FRB-sTurboID (C)

Plasmid

#153003

Purpose

Expresses low-affinity split-TurboID C-terminal fragment in mammalian cells

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID (C) (G74)

Use

Lentiviral

Tags

FRB, HA, and HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX302_FOXA1-V5

Plasmid

#70090

Purpose

Expression of human FOXA1 with C-terminal V5 tag, puromycin selection marker, 3rd generation lentiviral vector

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIP CMV GFP1-9

Plasmid

#130271

Purpose

Lentiviral expression vector for stable expression of GFP1-9

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Insert

GFP 1-9 OPT

Use

Lentiviral

Tags

none

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_SPI1_P2A_Hygro_Barcode

Plasmid

#120482

Purpose

Barcoded lentiviral vector to express SPI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SPI1 (SPI1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Plasmid

#172503

Purpose

Encodes the DisCas7-11 protein only for bacterial recombinant protein expression for purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
bRA89

Plasmid

#100950

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH marker

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK194

Plasmid

#59563

Purpose

TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP XbaI attB1 Kozak mKate opal attB2 AscI

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
tetO-NKX2.5

Plasmid

#46029

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

NKX2.5 (NKX2-5 Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Promoter

tetO

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF2859

Plasmid

#143386

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ID1 (ID1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.4 human alpha4 full

Plasmid

#221397

Purpose

Mammalian expression of human integrin alpha4 full length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin alpha4 full length (ITGA4 Human)

Tags

Strep twin

Expression

Mammalian

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 HA c-Jun

Plasmid

#237333

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Article

Rizzini et al Curr Biol. 2019 Jun 17;29(12):1954-1962.

Insert

c-Jun (JUN Human)

Tags

HA

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLCN DSB Repair Reporter (DRR)

Plasmid

#98895

Purpose

DSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

Neomycin-T2A-ISce1-ISce1-eGFP

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
aCKAR

Plasmid

#124427

Purpose

FRET-based reporter for monitoring aPKC activity in cells

Depositor

Alexandra Newton

Article

Kajimoto et al Sci Signal. 2019 Jan 1;12(562). pii: 12/

Insert

aCKAR

Tags

mCFP and mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-GMR-eya(shRNA)

Plasmid

#157991

Purpose

pBSII-KS(-)-based vector backbone containing a dominant eye marker for constructing Drosophila CRISPR HDR templates. Marker allows counter selection against vector integration events.

Depositor

Greg Beitel

Article

Nyberg et al Fly (Austin). 2020 Oct 5. doi: 10.1080/1

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pXW109Hg(RS-RinA-E11)2

Plasmid

#123151

Purpose

Mercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, PmerT, PhrpLE, PrinA

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMV306DIG13+FFlucRT

Plasmid

#49998

Purpose

Integrase removed to increase stability of the plasmid in mycobacteria

Depositor

Brian Robertson

Article

Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

Insert

G13 promoter + Firefly luciferase Red Thermostable

Use

Mycobacteria integrating vector

Expression

Bacterial

Mutation

Codon optimized for Mycobacterium tuberculosis wi…

Promoter

G13

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMV306G13+FFlucRT

Plasmid

#49997

Purpose

Integrating vector with an improved Red-shifted Thermostable Firefly Luciferase

Depositor

Brian Robertson

Article

Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

Insert

G13 promoter + Firefly luciferase Red Thermostable

Use

Mycobacteria integrating vector

Expression

Bacterial

Mutation

Codon optimized for Mycobacterium tuberculosis wi…

Promoter

G13 from M. marinum

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAYP (pDre)

Plasmid

#62709

Purpose

Mammalian expression of Dre recombinase from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

Dre

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF2745

Plasmid

#143605

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

HELZ2 (PRIC285 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge