19,627 results

1. pcDNA3 HA Arf1 DN T31N

   Plasmid

   #10833

   Depositor

   Thomas Roberts

   Article

   Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

   Insert

   Arf1 T31N (ARF1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T31N. Defective in GTP binding. Dominant negati…

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 HA Arf1 ActQ71L

   Plasmid

   #10832

   Depositor

   Thomas Roberts

   Article

   Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

   Insert

   Arf1 Q71L (ARF1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Q71L. Defective in GTP hydrolysis. Constitutive…

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-Sfp

   Plasmid

   #159617

   Purpose

   Expression plasmid for Sfp phosphopantetheinyl transferase

   Depositor

   Christopher T Walsh, Jun Yin

   Article

   Zhou et al ACS Chem Biol. 2007 May 22;2(5):337-46.

   Insert

   Sfp
   

   Tags

   6HIS

   Expression

   Bacterial

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. WT GBA pcDNA3.1

   Plasmid

   #188580

   Purpose

   Ectopic expression of wild-type GBA

   Depositor

   Anthony Schapira

   Article

   Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

   Insert

   GBA (GBA1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-KRAS-G12D

   Plasmid

   #116423

   Purpose

   Lentiviral expression of KRAS G12D

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   KRAS (KRAS Human)
   

   Use

   Lentiviral

   Mutation

   G12D

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 APEX2-NES

   Plasmid

   #49386

   Purpose

   Expresses APEX2 in the cytosol of mammalian cells

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   Flag - APEX2 - NES
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K14D, W41F, E112K, A134P on soybean APX; improved…

   Promoter

   CMV

   Available Since

   Nov. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-PGK-PYL1-sfGFP-Coilin

   Plasmid

   #122177

   Purpose

   expression of PYL1-SfGFP-Coilin, lentivirus vector

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   Coilin (COIL Human)
   

   Use

   Lentiviral

   Tags

   PYL1-SFGFP

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1.Gαi2-LgB91

   Plasmid

   #134358

   Purpose

   Nanoluc complementation assay. Expression of Gαi2 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi2. Addition of the HA epitope at N terminus of Gαi2.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gαi2-LgB91 (GNAI2 Human)
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Promoter

   T7

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. enAsABE8e

   Plasmid

   #138506

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-dAsCas12a (En)
   

   Expression

   Mammalian

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. mRNA-CleanCap entry vector

    Plasmid

    #188249

    Purpose

    Cloning vector for generating template plasmids to transcribe mRNA, with mNeonGreen dropout following BsaI digestion

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHIV-H2BmRFP

    Plasmid

    #18982

    Depositor

    Zena Werb, Bryan Welm

    Article

    Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

    Insert

    Histone H2B monomeric red fluorescent protein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. mEGFP-Rac1

    Plasmid

    #83950

    Purpose

    Rac1 FLIM donor for dynamic imaging of GTPase activity

    Depositor

    Ryohei Yasuda

    Article

    Hedrick et al Nature. 2016 Sep 28;538(7623):104-108. d

    Insert

    ras-related C3 botulinum toxin substrate 1 (RAC1 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH-EF1a-mCherry-EGFP-LC3B

    Plasmid

    #170446

    Purpose

    Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    mCherry-GFP-LC3 (MAP1LC3B Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

    Plasmid

    #108871

    Purpose

    Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

    Depositor

    Alex Schier

    Article

    Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

    Inserts

    5xU6:sgRNA
    Cas9-t2A-GFP
    

    Use

    CRISPR

    Promoter

    U6 and hsp70

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAID-EF1a linearizing in pX330

    Plasmid

    #140610

    Purpose

    Crispr/Cas9 plasmid for linearization of pAID-EF1a

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    sgRNA for pAID-EF1a plasmid digestion
    

    Use

    CRISPR

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-SFFV-PYL1-sfGFP-Emerin

    Plasmid

    #121532

    Purpose

    expression of PYL-SfGFP-Emerin, lentivirus vector

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    Emerin (EMD Human)
    

    Use

    Lentiviral

    Tags

    PYL1-SFGFP

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Fos-CreERT2

    Plasmid

    #194643

    Purpose

    Expresses Cre-ERT2 under the control of c-Fos promoter

    Depositor

    Jun-Hyeong Cho

    Article

    Lee et al Nat Neurosci. 2022 Dec 23. doi: 10.1038/

    Insert

    Fos-CreERT2
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMRX-INU-TEX264-FLAG

    Plasmid

    #128258

    Purpose

    Expresses TEX264 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWJ66

    Plasmid

    #46570

    Purpose

    Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    dcas9-w
    CRISPR array
    

    Use

    CRISPR

    Tags

    rpoZ

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Available Since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

    Plasmid

    #138150

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    hU6_Rfxcas13d-DR1_BsmBI
    

    Use

    Lentiviral

    Mutation

    DR1 A>T

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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