We narrowed to 18,979 results for: INO

1. retro-gRNA-eGFP

   Plasmid

   #116926

   Purpose

   Retrovirus expressing eGFP with BBSI cloning sites for sgRNA

   Depositor

   Diane Mathis, Christophe Benoist

   Article

   Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

   Insert

   eGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pmStrawberry-Atg4BC74A

   Plasmid

   #21076

   Depositor

   Tamotsu Yoshimori

   Article

   Fujita et al Mol Biol Cell. 2008 Nov . 19(11):4651-9.

   Insert

   Atg4B(C74A) (Atg4b Mouse)
   

   Use

   Tags

   mStrawberry

   Expression

   Mammalian

   Mutation

   220-222 TGC(Cys) is changed to GCA (Ala)

   Promoter

   Available since

   June 8, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3/mStayGold(c4)=UtrCH

   Plasmid

   #212020

   Purpose

   For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   UtrCH
   

   Use

   Tags

   mStayGold(c4)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PG13-luc (wt p53 binding sites)

   Plasmid

   #16442

   Purpose

   Luciferase reporter containing 13 copies of the p53-binding consensus sequence

   Depositor

   Bert Vogelstein

   Article

   el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

   Insert

   p53-binding site (TP53 )
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. sgPBRM1-1- LentiCRISPRv2

   Plasmid

   #107403

   Purpose

   Lentiviral expression of Cas9 and gRNA targeting PBRM1

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   gRNA targeting PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. sgPBRM1-2- LentiCRISPRv2

   Plasmid

   #107404

   Purpose

   Lentiviral expression of Cas9 and gRNA targeting PBRM1

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   gRNA targeting PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-hSyn-mScarlet

   Plasmid

   #131001

   Purpose

   AAV vector to drive the expression of mScarlet under the control of human Synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Has Service

   AAV1 and AAV8

   Insert

   mScarlet
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. STARR-seq luciferase validation vector_ORI_empty

   Plasmid

   #99297

   Purpose

   Luciferase validation vector; empty vector without insert to determine baseline

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Type

   Empty backbone

   Use

   Luciferase; Starr-seq luciferase validation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-Neo-Bam p53 R175H

   Plasmid

   #16436

   Depositor

   Bert Vogelstein

   Article

   Baker et al Science. 1990 Aug 24. 249(4971):912-5.

   Insert

   p53 R175H (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R175H

   Promoter

   CMV

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pS648∙CsR

    Plasmid

    #197860

    Purpose

    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    gRNA scaffold - Gm resistance cassette - incP oriT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PEM7

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-Ef1a-DIO mScarlet

    Plasmid

    #131002

    Purpose

    Double floxed mScarlet under the control of Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV1 and AAV5

    Insert

    mScarlet
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. VEGFR2-mCh

    Plasmid

    #108854

    Purpose

    Encodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linker

    Depositor

    Kalina Hristova

    Article

    Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Labeled with mCherry on the C-Terminus via a 3 am…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. L2125-FRB-GPA-NLuc-CyOFP1

    Plasmid

    #222885

    Purpose

    Positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-20GS-NanoLuc-10GS-CyOFP1
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMRX-IBU-pSu9-HaloTag7-SNAP

    Plasmid

    #217319

    Purpose

    Expresses the presequence of Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag for measuring mitophagy activity by Halo processing assay.

    Depositor

    Noboru Mizushima

    Article

    Yang et al EMBO J. 2024 Nov;43(22):5613-5634. doi:

    Insert

    Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag
    

    Use

    Retroviral

    Tags

    HaloTag and SNAP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

    Plasmid

    #201916

    Purpose

    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-Cas9 v2
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and Human U6

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAVS1-P-CAG-GFP

    Plasmid

    #80491

    Purpose

    donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    CAG-GFP
    

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-PIK3C2A

    Plasmid

    #161988

    Purpose

    Mammalian expression vector containing PIK3C2A tagged with GFP

    Depositor

    Volker Haucke, David Bryant

    Article

    Posor et al Nature. 2013 Jul 11;499(7457):233-7. doi

    Insert

    PIK3C2A (PIK3C2A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. lentiCRISPRi(v2)-Blast

    Plasmid

    #170068

    Purpose

    Expresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistance

    Depositor

    Neville Sanjana

    Article

    Morris et al Science. 2023 May 19;380(6646):eadh7699.

    Insert

    KRAB-dCas9-MeCP2
    

    Use

    CRISPR and Lentiviral

    Tags

    2A and 2xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAY5 (mAID-EGFP-NLS piggyBac)

    Plasmid

    #140532

    Purpose

    mAID-EGFP-NLS piggyBac

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    mAID-EGFP-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBa.M6PR-GFP

    Plasmid

    #224407

    Purpose

    Mammalian expression of M6PR

    Depositor

    Marvin Bentley

    Article

    Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

    Insert

    M6PR (M6PR Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMA3383

    Plasmid

    #46875

    Purpose

    A retroviral vector for the constitutive expression of soluble guanylate cyclase1 beta3

    Depositor

    Mikhail Alexeyev, Troy Stevens

    Article

    Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

    Insert

    soluble guanylate cyclase 1beta 3 (Gucy1b3 Rat)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    LTR

    Available since

    Aug. 13, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pCMV-Flag-hGSDMD

    Plasmid

    #218938

    Purpose

    Expression of N-terminal FLAG tagged human gasdermin D

    Depositor

    Christopher LaRock

    Article

    LaRock et al Nature. 2022 May;605(7910):527-531. doi:

    Insert

    human GSDMD (GSDMD Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBabe B-Raf V600E

    Plasmid

    #17544

    Depositor

    Channing Der

    Article

    Hao et al Mol Cancer Ther. 2007 Aug . 6(8):2220-9.

    Insert

    B-Raf (BRAF Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    V600E

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. VPS13B^GFP

    Plasmid

    #224588

    Purpose

    codon optimized GFP tagged VPS13B for expression in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

    Insert

    VPS13B^GFP (VPS13B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-Neo-Bam p53 R273H

    Plasmid

    #16439

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R273H (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R273H

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-Neo-Bam p53 R248W

    Plasmid

    #16437

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R248W (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R248W

    Promoter

    Available since

    Aug. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. U6-NT sgRNA; EF1a-dCas9-KRAB-GFP

    Plasmid

    #194284

    Purpose

    EF1a-dCas9-KRAB-GFP with nontarget sgRNA

    Depositor

    Elizabeth Kirby

    Article

    Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

    Insert

    dCas9-KRAB-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pN251

    Plasmid

    #188471

    Purpose

    Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)

    Depositor

    Le Cong

    Article

    Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

    Insert

    enCas12a-HF (gift from Dr. Keith Joung)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLKO-CreERT2 stuffer v3

    Plasmid

    #158046

    Purpose

    lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pLenti-hPGK-eGFP-SynaptoZip

    Plasmid

    #122523

    Purpose

    Lentiviral expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by hPGK promoter

    Depositor

    Antonio Malgaroli

    Article

    Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

    Insert

    eGFP-SynaptoZip (Vamp2 Rat)
    

    Use

    Lentiviral

    Tags

    Myc and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBa. BDNF-Halo

    Plasmid

    #162718

    Purpose

    Mammalian expression of rat BDNF

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    Brain derived neurotrophic factor (Bdnf Rat)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Chicken Beta actin

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLKO human ULK2 shRNA 91

    Plasmid

    #27634

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    human ULK2 shRNA 91
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

33. pcDNA3 HA E2F3B

    Plasmid

    #236438

    Purpose

    transient overexpression of E2F3B in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F3B (E2F3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLKO.1-puro_CMV_NES-Caprola_06-mEGFP

    Plasmid

    #194693

    Purpose

    CMV driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through through lentivirus transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_06-mEGFP
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLKO.1-puro_CMV_NES-Caprola_off-mEGFP

    Plasmid

    #194695

    Purpose

    CMV driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through lentivirus transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_off-mEGFP
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CRISPR-StAR4 (Retro)

    Plasmid

    #222690

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    PGK without BbsI cutsite

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRK5-FLAG-p14

    Plasmid

    #42330

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

    Insert

    p14 (LAMTOR2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAVS1-P-CAG-GFPluc2

    Plasmid

    #80493

    Purpose

    donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expression

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    GFPluc2
    

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJL1

    Plasmid

    #69496

    Purpose

    pJL1 expression vector with sfGFP

    Depositor

    Michael Jewett

    Article

    Martin et al Nat Commun. 2018 Mar 23;9(1):1203. doi:

    Insert

    Super folder GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJRH051

    Plasmid

    #171625

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a and U6

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pRK5 c-Fyn

    Plasmid

    #16032

    Depositor

    Filippo Giancotti

    Article

    Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

    Insert

    c-Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. GST-Delta23C11orf83

    Plasmid

    #65849

    Purpose

    bacterial expression of GST (N-ter) - Delta 23 C11orf83/UQCC3 (UQCC3 protein depleted of its N-terminal transmembrane domain)

    Depositor

    Amos Bairoch

    Article

    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

    Insert

    UQCC3 depleted of its N terminal transmembrane part (UQCC3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deletion of the 23 first aa (TM)

    Promoter

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. p23-NES-RfxCas13d-msfGFP-NES-Flag

    Plasmid

    #165076

    Purpose

    overexpression of RfxCas13d in human cells

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    RfxCas13d
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pSH624-ssr

    Plasmid

    #199241

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    single-stranded DNA recombineering
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq-Ptrc

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pS548∙CsR

    Plasmid

    #197859

    Purpose

    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    gRNA scaffold - Tc resistance cassette - incP oriT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PEM7

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. Mito-CanlonicSF/pCMV-myc-mito

    Plasmid

    #224465

    Purpose

    To explore mitochondrial lactate dynamics

    Depositor

    Alejandro San Martín

    Article

    Rauseo et al bioRxiv 2024.07.19.604361

    Insert

    CanlonicSF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

47. AAT-PB-CD2APtk

    Plasmid

    #86004

    Purpose

    homology donor for AAT locus with piggyBac-flanked DsRedEx_T2A_puroTK cassette

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

    Insert

    DsRedEx_T2A_puro_dTK
    

    Use

    Piggybac exciseable

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pBABE-puro SHP2 WT

    Plasmid

    #8329

    Depositor

    Ben Neel

    Article

    Zhang et al Mol Cell Biol 2002 Jun;22(12):4062-72.

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pDule-pCNF

    Plasmid

    #85494

    Purpose

    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

    Insert

    para-cyanophenylalanine Mj synthetase
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    lpp (constitutive)

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pTACR TH1-p2aGFP

    Plasmid

    #135815

    Purpose

    CRISPR based on px330 p2aGFP targeting TH gene near stop codon

    Depositor

    Lee Rubin, Tim Ahfeldt

    Article

    Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

    Insert

    TH 1 guide
    

    Use

    CRISPR

    Tags

    p2a Myr-eGFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge