We narrowed to 8,363 results for: construct

1. C234-E07: attB2r-HA-attB3

   Plasmid

   #162911

   Purpose

   Gateway attB2r/attB3 entry clone for construction of C-terminal HA epitope tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   HA epitope tag
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. C234-E06: attB2r-FLAG-attB3

   Plasmid

   #162910

   Purpose

   Gateway attB2r/attB3 entry clone for construction of C-terminal FLAG epitope tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   FLAG epitope tag
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. C234-E05: attB2r-V5-attB3

   Plasmid

   #162909

   Purpose

   Gateway attB2r/attB3 entry clone for construction of C-terminal V5 epitope tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   V5 epitope tag
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pVG97

   Plasmid

   #164849

   Purpose

   Plasmid for replacement of PEST sequence in strains with pr-yEVenus-PEST-ADH1t construct

   Depositor

   Sahand Jamal Rahi

   Article

   Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

   Insert

   Part of URA3 5' UTR and yEVenus with stop codon for recombination with pr-yEVenus-PEST-ADH1t:URA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNTI743 dual-barcoded gRNA parent vector

   Plasmid

   #164915

   Purpose

   CEN/ARS Parent vector for constructing barcoded-gRNA CiBER-seq plasmid libraries

   Depositor

   Nicholas Ingolia

   Article

   Muller et al Science. 2020 Dec 11;370(6522). pii: 370

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT2U2-YFP

   Plasmid

   #84544

   Purpose

   Hybrid reporter construct with 4 transcription factor binding sites (2 TetO, 2 UAS) driving EYFP

   Depositor

   Joshua Leonard

   Article

   Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

   Insert

   T2U2-EYFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-min

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pT2U3-YFP

   Plasmid

   #84547

   Purpose

   Hybrid reporter construct with 5 transcription factor binding sites (2 TetO, 3 UAS) driving EYFP

   Depositor

   Joshua Leonard

   Article

   Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

   Insert

   T2U3-EYFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-min

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pU2T4-YFP

   Plasmid

   #84548

   Purpose

   Hybrid reporter construct with 6 transcription factor binding sites (2 UAS, 4 TetO) driving EYFP

   Depositor

   Joshua Leonard

   Article

   Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

   Insert

   U2T4-EYFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-min

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pT2U2T2-YFP

   Plasmid

   #84549

   Purpose

   Hybrid reporter construct with 6 transcription factor binding sites (2 TetO, 2 UAS, 2 TetO) driving EYFP

   Depositor

   Joshua Leonard

   Article

   Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

   Insert

   T2U2T2-EYFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-min

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pU2T4U2-YFP

    Plasmid

    #84551

    Purpose

    Hybrid reporter construct with 8 transcription factor binding sites (2 UAS, 4 TetO, 2 UAS) driving EYFP

    Depositor

    Joshua Leonard

    Article

    Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

    Insert

    U2T4U2-EYFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-min

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pT2U4T2-YFP

    Plasmid

    #84553

    Purpose

    Hybrid reporter construct with 8 transcription factor binding sites (2 TetO, 4 UAS, 2 TetO) driving EYFP

    Depositor

    Joshua Leonard

    Article

    Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

    Insert

    T2U4T2-EYFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-min

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU2T2-YFP

    Plasmid

    #84543

    Purpose

    Hybrid reporter construct with 4 transcription factor binding sites (2 UAS, 2 TetO) driving EYFP

    Depositor

    Joshua Leonard

    Article

    Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

    Insert

    U2T2-EYFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-min

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCTCON2-Aga1p-FAPB2.3.6L1TAG

    Plasmid

    #158143

    Purpose

    Yeast display reporter for non-display yeast strains (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    FAPB2.3.6L1TAG
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCTCON2-GXB

    Plasmid

    #160926

    Purpose

    Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Inserts

    BFP
    sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEPOR2KN0048

    Plasmid

    #117611

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for pEPOR1CB0012(pEPOR1CB0012) and sgRNA_Rpn2/Psmd1 subunit {AT2G32730}(pEPOR1CB0075)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:pEPOR1CB0012(pEPOR1CB0012) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNOC-stacked-GeNL-Ca

    Plasmid

    #101003

    Purpose

    Construct for fusion expression of GeNL calcium reporter and neomycin/G418 resistance gene by a bidirectional promoter

    Depositor

    Eva Farre

    Article

    Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUG-125

    Plasmid

    #104636

    Purpose

    Level 2 construct

    Depositor

    Marco Trujillo

    Article

    Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

    Insert

    HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB-F/A_EL4
    

    Use

    Tags

    HIS-Ubq, HA-E2, GST-E3

    Expression

    Bacterial

    Mutation

    AvrPtoB-Phe479Ala

    Promoter

    T7

    Available since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUG-413

    Plasmid

    #104639

    Purpose

    Level 2 construct

    Depositor

    Marco Trujillo

    Article

    Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

    Insert

    HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB-F/A_Myc-Fen_EL5
    

    Use

    Tags

    HIS-Ubq, HA-E2, GST-E3

    Expression

    Bacterial

    Mutation

    AvrPtoB-Phe479Ala

    Promoter

    T7

    Available since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEPOR2KN0063

    Plasmid

    #117630

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_pEPOR1CB0089(pEPOR1CB0089)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEPOR2KN0062

    Plasmid

    #117629

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113) and sgRNA_UUAT1 {AT5G04160}(pEPOR1CB0087)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEPOR2KN0061

    Plasmid

    #117628

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_UUAT1 {AT5G04160}(pEPOR1CB0087)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEPOR2KN0057

    Plasmid

    #117624

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtCPK34 {AT5G19360}(pEPOR1CB0084)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEPOR2KN0053

    Plasmid

    #117620

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_Methyltransferase {AT5G10620}(pEPOR1CB0083)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEPOR2KN0052

    Plasmid

    #117619

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for pEPOR1CB0012(pEPOR1CB0012) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:pEPOR1CB0012(pEPOR1CB0012) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEPOR2KN0032

    Plasmid

    #117604

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0027)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEPOR2KN0017

    Plasmid

    #117589

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-KA(pEPOR1CB0009) and sgRNA_NbPDS(pEPOR1CB0114)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-KA(pEPOR1CB0009) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEPOR2KN0012

    Plasmid

    #117584

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS(pEPOR1CB0114)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-DE(pEPOR1CB0008) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEPOR2KN0004

    Plasmid

    #117579

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0023)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEPOR2KN0003

    Plasmid

    #117578

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0022)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEPOR2KN0002

    Plasmid

    #117577

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas-p(pEPOR1CB0002) and sgRNA_NbPDS(pEPOR1CB0114)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas-p(pEPOR1CB0002) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEPOR2KN0001

    Plasmid

    #117576

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0114)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEPOR2KN0049

    Plasmid

    #117616

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSO5

    Plasmid

    #106364

    Purpose

    A vector backbone, including mKate, to be used to make constructs to be inserted as a single copy at a site on Chr I.

    Depositor

    Jonathan Ewbank

    Article

    Lee et al Virulence. 2018 Dec 31;9(1):648-658. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAGH50

    Plasmid

    #107254

    Purpose

    Vipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor away Vipp1 (sll0617) of Synecocystis PCC6803.

    Depositor

    Erin O'Shea

    Article

    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

    Insert

    vipp1-gfp-fkbp12
    

    Use

    Suicide vector for destined for double homologous…

    Tags

    GFP and FKBP12

    Expression

    Bacterial

    Mutation

    Promoter

    native

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. -24lacZ

    Plasmid

    #25422

    Depositor

    David J. Goldhamer

    Article

    Chen et al Dev Dyn. 2001 Jul . 221(3):274-88.

    Insert

    Myo D 5' flanking sequence (MYOD1 Human)
    

    Use

    Reporter construct

    Tags

    lacZ

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

36. pWJPrP31

    Plasmid

    #22105

    Depositor

    Susan Lindquist

    Article

    Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

    Insert

    PrP exon 3 EcoR1 fragment
    

    Use

    Construction of targeting vector

    Tags

    Expression

    Mutation

    genomic EcoR1 fragment containing exon 3 of PrP

    Promoter

    Available since

    Oct. 6, 2009

    Availability

    Academic Institutions and Nonprofits only

37. pWJPrP34

    Plasmid

    #22107

    Depositor

    Susan Lindquist

    Article

    Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

    Insert

    PrP exon 3 EcoR1 fragment
    

    Use

    Construction of targeting vector

    Tags

    Expression

    Mutation

    Added a Cla1 site right before translation start …

    Promoter

    Available since

    Oct. 6, 2009

    Availability

    Academic Institutions and Nonprofits only

38. pLys-M1 backbone

    Plasmid

    #109386

    Purpose

    Empty plasmid of the pLys-M1 construct. This is medium copy (p15A), CmR

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV_CKAR3

    Plasmid

    #238413

    Purpose

    AAV construct with CKAR3 expression under a human synapsin promotor and WPRE3 translation enhancer.

    Depositor

    Haining Zhong

    Article

    Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

    Insert

    CKAR3 (PKC sensor)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PEP108E

    Plasmid

    #97394

    Purpose

    binary construct of plasma membrane-targeted sfGFP1-10 for plant expression

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    sfGFP1-10OPT
    

    Use

    Tags

    Plasma membrane target

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. yTREX

    Plasmid

    #177306

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

    Depositor

    Karl-Erich Jaeger

    Article

    Domrose et al Synth Syst Biotechnol. 2017 Nov 15;2(4):

    Type

    Empty backbone

    Use

    Synthetic Biology; Yeast expression, tn5 genomic …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pWM_12x601_25bpLinker

    Plasmid

    #157788

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_25bp linker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAIDA1

    Plasmid

    #79180

    Purpose

    A construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tags

    Depositor

    Gen Larsson

    Article

    Jarmander et al Microb Cell Fact. 2012 Sep 3;11:118. doi

    Insert

    Adhesin Involved in Diffuse Adherence
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pmScarlet3-alphaTubulin_C1

    Plasmid

    #189768

    Purpose

    Construct for labelling microtubules in mammalian cells with mScarlet3-alphatubulin

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3-alphaTubulin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. TetOn-mCherry-eGFP-RAMP4

    Plasmid

    #109014

    Purpose

    Tet-On construct to induce expression of tandem eGFP-mCherry tagged RAMP4 (ER marker)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    RAMP4 (SERP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pMF-FU_TetO_GW_PPARG2

    Plasmid

    #193289

    Purpose

    Lentiviral construct that expresses a dox-inducible human PPARG2

    Depositor

    Rudolf Jaenisch

    Article

    Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

    Insert

    PPARG2 (PPARG2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. L304-EGFP-Tubulin-WT

    Plasmid

    #64060

    Purpose

    EGFP fused at the N-terminus of tubulin; lentiviral construct

    Depositor

    Weiping Han

    Article

    Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

    Insert

    tubulin
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. GLUT1-GFP

    Plasmid

    #89440

    Purpose

    GLUT1 construct

    Depositor

    Jeffrey Rathmell

    Article

    Wieman et al Biochem J. 2009 Mar 1;418(2):345-67. doi

    Insert

    GLUT1 (Slc2a1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pmCherry-C1 mCherry-NLS

    Plasmid

    #58476

    Purpose

    Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Insert

    3X NLS with 3X stop codons
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. TRE-mCherry-p2A-eNpHR3.0-TS

    Plasmid

    #158703

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    mCherry-p2A-eNpHR3.0-TS
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge