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Showing: 5351 - 5400 of 33749 results

  3. pDONOR-MARINA

    Plasmid
    #223328
    Purpose
    DONOR vector for genomic integration of MARINA voltage sensitive indicator gene from Platisa et al., 2017 (10.1021/acschemneuro.6b00234).
    Depositor
    Jonathan Arias
    Article
    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do
    Inserts
    MARINA
    KIR2.1 channel Golgi-to-plasma membrane trafficking signal
    Use
    CRISPR and Synthetic Biology
    Tags
    mCherry and super-ecliptic pHluorin
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    223328-plasmid-map-sequence-id-447745

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  4. pTwist-SARS-CoV-2 Spike-deltaC18 G142D (GAT)+deltaV143-Y145

    Plasmid
    #212459
    Purpose
    Encodes SARS-CoV-2 spike G142D, V143-Y145 deletion for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike G142D, V143-Y145 deletion (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    G142D, V143-Y145 deletion
    Promoter
    CAG
    Available Since
    Sept. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212459-plasmid-map-sequence-id-447107

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  5. pTwist-SARS-CoV-2 Spike-deltaC18 S371F (TTC) S373P (CCA) S375F (TTC) T376A (GCT)

    Plasmid
    #212493
    Purpose
    Encodes SARS-CoV-2 spike S371F, S373P, S375F, T376A for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike S371F, S373P, S375F, T376A (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    S371F, S373P, S375F, T376A
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212493-plasmid-map-sequence-id-432812

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  6. pTwist-SARS-CoV-2 Spike-deltaC18 Q493R (CGA) Q498R (CGA) N501Y (TAT) Y505H (CAT)

    Plasmid
    #212498
    Purpose
    Encodes SARS-CoV-2 spike Q493R, Q498R, N501Y, Y505H for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike Q493R, Q498R, N501Y, Y505H (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    Q493R, Q498R, N501Y, Y505H
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212498-plasmid-map-sequence-id-432816

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  7. pTwist-SARS-CoV-2 Spike-deltaC18 S371L (CTC) S373P (CCA) S375F (TTC)

    Plasmid
    #212467
    Purpose
    Encodes SARS-CoV-2 spike S371L, S373P, S375F for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike S371L, S373P, S375F (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    S371L, S373P, S375F
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212467-plasmid-map-sequence-id-432794

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  8. pTwist-SARS-CoV-2 Spike-deltaC18 S477N (AAC) T478K (AAA) E484A (GCA)

    Plasmid
    #212472
    Purpose
    Encodes SARS-CoV-2 spike S477N, T478K, E484A for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike S477N, T478K, E484A (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    S477N, T478K, E484A
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212472-plasmid-map-sequence-id-432798

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  9. pTwist-SARS-CoV-2 Spike-deltaC18 N211I (ATT) deltaL212+InsertionR214-EPE

    Plasmid
    #212462
    Purpose
    Encodes SARS-CoV-2 spike N211I, L212 deletion, R214 insertion for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike N211I, L212 deletion, R214 insertion (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    N211I, L212 deletion, R214 insertion
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212462-plasmid-map-sequence-id-432791

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  10. pTwist-SARS-CoV-2 Spike-deltaC18 B.1.621 (Mu)

    Plasmid
    #212452
    Purpose
    Encodes SARS-CoV-2 spike T95I ,YY144TSN ,R346K ,E484K ,N501Y ,D614G ,P681H for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike T95I ,YY144TSN ,R346K ,E484K ,N501Y ,D614G ,P681H (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    T95I ,YY144TSN ,R346K ,E484K ,N501Y ,D614G ,P681H
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212452-plasmid-map-sequence-id-432785

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  12. pTwist-SARS-CoV-2 Spike-deltaC18 K417N (AAT) L452R (CGT) T478K (AAA) E484K (AAA)

    Plasmid
    #212439
    Purpose
    Encodes SARS-CoV-2 spike K417N, L452R, T478K, E484K for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike K417N, L452R, T478K, E484K (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    K417N, L452R, T478K, E484K
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212439-plasmid-map-sequence-id-432776

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  13. pTwist-SARS-CoV-2 Spike-deltaC18 deltaR246-G252+D253N (AAC)

    Plasmid
    #212444
    Purpose
    Encodes SARS-CoV-2 spike R246-G252 deletion, D253N for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike R246-G252 deletion, D253N (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    R246-G252 deletion, D253N
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212444-plasmid-map-sequence-id-433151

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  14. pTwist-SARS-CoV-2 Spike-deltaC18 C.37

    Plasmid
    #212448
    Purpose
    Encodes SARS-CoV-2 spike G75V ,T76I ,deltaR246-G252+D253N ,L452Q ,F490S ,D614G ,T859N for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike G75V ,T76I ,deltaR246-G252+D253N ,L452Q ,F490S ,D614G ,T859N (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    G75V ,T76I ,deltaR246-G252+D253N ,L452Q ,F490S ,D…
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212448-plasmid-map-sequence-id-432781

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  15. pTwist-SARS-CoV-2 Spike-deltaC18 B.1.617v4 (B.1.617.2 v1)

    Plasmid
    #212430
    Purpose
    Encodes SARS-CoV-2 spike T19R,K77T,G142D,L452R,T478K,D614G,P681R,D950N for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike T19R,G142D,L452R,T478K,D614G,P681R,D950N (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    T19R,G142D,L452R,T478K,D614G,P681R,D950N
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212430-plasmid-map-sequence-id-433150

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  16. pTwist-SARS-CoV-2 Spike-deltaC18 K417N (AAT) L452R (CGT) T478K (AAA)

    Plasmid
    #212438
    Purpose
    Encodes SARS-CoV-2 spike K417N, L452R, T478K for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike K417N, L452R, T478K (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    K417N, L452R, T478K
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212438-plasmid-map-sequence-id-432775

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  17. pTwist-SARS-CoV-2 Spike-deltaC18 B.1.617v1 (B.1.617.1 v1)

    Plasmid
    #212428
    Purpose
    Encodes SARS-CoV-2 spike G142D,E154K,L452R,E484Q,D614G,P681R,Q1071H,H1101D for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike G142D,E154K,L452R,E484Q,D614G,P681R,Q1071H,H1101D (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    G142D,E154K,L452R,E484Q,D614G,P681R,Q1071H,H1101D
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212428-plasmid-map-sequence-id-432768

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  18. pTwist-SARS-CoV-2 Spike-deltaC18 B.1.526v1

    Plasmid
    #212415
    Purpose
    Encodes SARS-CoV-2 spike L5F,T95I,D253G,S477N,D614G,Q957R for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike L5F,T95I,D253G,S477N,D614G,Q957R (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    L5F,T95I,D253G,S477N,D614G,Q957R
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212415-plasmid-map-sequence-id-431647

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  19. pTwist-SARS-CoV-2 Spike-deltaC18 B.1.526v2

    Plasmid
    #212416
    Purpose
    Encodes SARS-CoV-2 spike L5F,T95I,D253G,E484K,D614G,A701V for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike L5F,T95I,D253G,E484K,D614G,A701V (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    L5F,T95I,D253G,E484K,D614G,A701V
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212416-plasmid-map-sequence-id-431648

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  20. pTwist-SARS-CoV-2 Spike-deltaC18 (Bluebird)

    Plasmid
    #212406
    Purpose
    Encodes SARS-CoV-2 spike T29I,T572I,D614G,Q677H,D936N for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike T29I,T572I,D614G,Q677H,D936N (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    T29I,T572I,D614G,Q677H,D936N
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212406-plasmid-map-sequence-id-431639

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  21. pTwist-SARS-CoV-2 Spike-deltaC18 (B.1.525)

    Plasmid
    #212398
    Purpose
    Encodes SARS-CoV-2 spike Q52R,A67V,Y145 deletion,E484K,D614G,Q677H,F888L for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike Q52R,A67V,Y145 deletion,E484K,D614G,Q677H,F888L (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    Q52R,A67V,Y145 deletion,E484K,D614G,Q677H,F888L
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212398-plasmid-map-sequence-id-431635

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  22. pTwist-SARS-CoV-2 Spike-deltaC18 (P.1) - No RBD Mutations

    Plasmid
    #212384
    Purpose
    Encodes SARS-CoV-2 spike L18F,R21N,P26S,D138Y,R190S,D614G,H655Y,T1027I,V1176F for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike L18F,R21N,P26S,D138Y,R190S,D614G,H655Y,T1027I,V1176F (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    L18F,R21N,P26S,D138Y,R190S,D614G,H655Y,T1027I,V11…
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212384-plasmid-map-sequence-id-431624

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  23. pTwist-SARS-CoV-2 Spike-deltaC18 SA501.V2 (Triple RBD) R683G (GGC)

    Plasmid
    #212387
    Purpose
    Encodes SARS-CoV-2 spike K417N,E484K,N501Y,R683G for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike K417N,E484K,N501Y,R683G (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    K417N,E484K,N501Y,R683G
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212387-plasmid-map-sequence-id-431626

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  24. pTwist-SARS-CoV-2 Spike-deltaC18 VOC-501Y.V2 (B.1.351) - McGuire Mutations

    Plasmid
    #212388
    Purpose
    Encodes SARS-CoV-2 spike D80A,D215G,K417N,E484K,N501Y,D614G for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike D80A,D215G,K417N,E484K,N501Y,D614G (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    D80A,D215G,K417N,E484K,N501Y,D614G
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212388-plasmid-map-sequence-id-431627

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  25. pTwist-SARS-CoV-2 Spike-deltaC18 T307I (ATA) T572I (ATC) D614G (GGT)

    Plasmid
    #212377
    Purpose
    Encodes SARS-CoV-2 spike T307I, T572I, D614G for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike T307I, T572I, D614G (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    T307I, T572I, D614G
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212377-plasmid-map-sequence-id-431618

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  26. pTwist-SARS-CoV-2 Spike-deltaC18 D614G (GGT) Q677H (CAT) D936Y (TAT)

    Plasmid
    #212379
    Purpose
    Encodes SARS-CoV-2 spike D614G, Q677H, D936Y for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike D614G, Q677H, D936Y (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    D614G, Q677H, D936Y
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212379-plasmid-map-sequence-id-431620

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  27. pTwist-SARS-CoV-2 Spike-deltaC18 Danish Mink deltaFVI

    Plasmid
    #212372
    Purpose
    Encodes SARS-CoV-2 spike deltaH69-H70, Y453F, D614G, I692V, M1229I for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike deltaH69-H70, Y453F, D614G, I692V, M1229I (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    deltaH69-H70, Y453F, D614G, I692V, M1229I
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212372-plasmid-map-sequence-id-431614

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  28. pTwist-SARS-CoV-2 Spike-deltaC18 deltaL242-L244+R246I (ATA)

    Plasmid
    #212357
    Purpose
    Encodes SARS-CoV-2 spike deltaL242-L244, R246I for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike deltaL242-L244, R246I (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    deltaL242-L244, R246I
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212357-plasmid-map-sequence-id-431604

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  29. pTwist-SARS-CoV-2 Spike-deltaC18 SAK005321 (Wrong L242H)

    Plasmid
    #212350
    Purpose
    Encodes SARS-CoV-2 spike D80A, D215G, L242H, K417N, E484K, N501Y, D614G, A701V for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike D80A, D215G, L242H, K417N, E484K, N501Y, D614G, A701V (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    D80A, D215G, L242H, K417N, E484K, N501Y, D614G, A…
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212350-plasmid-map-sequence-id-431600

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  30. pCAG-lox-inv[Talpha1-HA-Dre]-lox-mOrange2-NEDD1-gTBD

    Plasmid
    #196894
    Purpose
    Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange aimed to displace endogenous gamma-TuRC from the centrosome. Used in combination with Talpha1-iCre-pA plasmids
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    N-gTBD-mOrange2 (NEDD1 Human)
    Use
    Cre/Lox; Dre/rox
    Expression
    Mammalian
    Promoter
    Quimeric CAG
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196894-plasmid-map-sequence-id-392229

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  31. pBactin-AcGFP-Cdk5rap2-CTDdeltaCBD

    Plasmid
    #196856
    Purpose
    Expression of the centrosome targeting carboxy-terminal domain (CTD) laking the centrosome binding domain (deltaCBD) of Cdk5rap2 fused to AcGFP. It cannot displace Cdk5rap2 from centrosomes
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    AcGFP-Cdk5rap2-CTDdeltaCBD (Cdk5rap2 Rat)
    Expression
    Mammalian
    Mutation
    Lacking the centrosome-targeting domain (CTD)
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196856-plasmid-map-sequence-id-391815

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  33. R620-M19-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP K417T/E484K/N501Y

    Plasmid
    #170203
    Purpose
    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant
    Depositor
    Dominic Esposito
    Article
    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.
    Insert
    SARS-CoV-2 RBD (S SARS-CoV-2)
    Tags
    3C-His8-SBP
    Expression
    Mammalian
    Mutation
    K417T, E484K, N501Y
    Promoter
    CMV51
    Available Since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170203-plasmid-map-sequence-id-334219

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  34. R620-M05-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 K417N/E484K/N501Y

    Plasmid
    #170191
    Purpose
    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant
    Depositor
    Dominic Esposito
    Article
    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.
    Insert
    SARS-CoV-2 RBD (S SARS-CoV-2)
    Tags
    His6
    Expression
    Mammalian
    Mutation
    K417N, E484K, N501Y
    Promoter
    CMV51
    Available Since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170191-plasmid-map-sequence-id-334254

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  35. SIN40C.EFS.eGFP.mArid3a-3'UTR-Mut

    Plasmid
    #169298
    Purpose
    Constitutive overexpression a mutated 3'UTR of the murine Arid3a gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.
    Depositor
    Jan-Henning Klusmann
    Article
    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0
    Insert
    Arid3a-3'UTR-Mut (Arid3a Mouse)
    Use
    Lentiviral
    Mutation
    Mutations in miR-125b-2 and let-7c binding sites
    Promoter
    EFS
    Available Since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169298-plasmid-map-sequence-id-337864

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  36. SIN40C.EFS.eGFP.ARID3A-3'UTR-Mut

    Plasmid
    #169296
    Purpose
    Constitutive overexpression a mutated 3'UTR of the human ARID3A gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.
    Depositor
    Jan-Henning Klusmann
    Article
    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0
    Insert
    ARID3A-3'UTR-Mut (ARID3A Human)
    Use
    Lentiviral
    Mutation
    Mutations in miR-125b-2 and let-7c binding sites
    Promoter
    EFS
    Available Since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169296-plasmid-map-sequence-id-337837

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  38. pEN487 - Sororin-AID[71-114]-eGFP-FRT-Blast-FRT targeting conuct

    Plasmid
    #156433
    Purpose
    Targeting vector to introduce an AID-eGFP cassette at the mouse Cdca5 (SORORIN) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA GGGATGCCCGTCATTAAGTG
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    SORORIN, AID (Cdca5 Mouse)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    none
    Available Since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    156433-plasmid-map-sequence-id-310157

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  39. pAAV.hSynap.iAChSnFR-Venus-NULL

    Plasmid
    #137953
    Purpose
    Expresses iAChSnFR (non-binding yellow version) under hSynapsin promoter
    Depositor
    Loren Looger
    Article
    Borden et al bioRxiv 2020
    Insert
    iAChSnFR (yellow version, non-binding variant)
    Use
    AAV
    Expression
    Mammalian
    Mutation
    cpSFGFP replaced with cpSFVenus; Y140A binding si…
    Promoter
    hSynapsin
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    137953-plasmid-map-sequence-id-271684

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  44. pCW-eGFP-FHA

    Plasmid
    #114129
    Purpose
    Lentiviral vector for inducible expression of eGFP-RNF8 FHA domain fusion; 53BP1-independent recruitment to damaged chromatin
    Depositor
    Daniel Durocher
    Article
    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:
    Insert
    RNF8 FHA domain (amino acids 2-160) (RNF8 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    aa 2-160
    Promoter
    TRE promoter, Tet ON
    Available Since
    Aug. 28, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114129-plasmid-map-sequence-id-220207

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  45. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

    Plasmid
    #132393
    Purpose
    Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site
    Depositor
    Gerold Schmitt-Ulms
    Article
    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:
    Insert
    human TauP301L 2N4R-EGFP (MAPT Human, Synthetic)
    Use
    CRISPR and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Proline 301 to Leucine (P301L)
    Promoter
    TREtight
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    132393-plasmid-map-sequence-id-341619

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  47. pAd-M3cherry

    Plasmid
    #61041
    Purpose
    This plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.
    Depositor
    Qiao Zhou
    Article
    Li et al Nat Biotechnol. 2014 Dec;32(12):1223-30.
    Inserts
    Ngn3 (Neurog3 Mouse)
    pdx1 (Pdx1 Mouse)
    Mafa (Mafa Mouse)
    monomeric cherry
    Use
    Adenoviral
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    61041-plasmid-map-sequence-id-456476

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  48. pTwist-SARS-CoV-2 Δ18 B.1.617.2v1

    Plasmid
    #179905
    Purpose
    Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014
    Insert
    SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    T19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…
    Promoter
    CAG
    Available Since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    179905-plasmid-map-sequence-id-351283

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  49. pAU002 - pTRE3G-CTCF(DELTA1-265)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid
    #156429
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    DELTA1-265
    Available Since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    156429-plasmid-map-sequence-id-310209

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Showing: 5351 - 5400 of 33749 results