We narrowed to 5,565 results for: SPI

1. pEBB-II-I-GFP beta isoform

   Plasmid

   #22147

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pEBG-TFII-I alpha

   Plasmid

   #22154

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Tags

   GST and His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pEBG-TFII-I beta

   Plasmid

   #22155

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Tags

   GST and His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pEBG-TFII-I deltaBR

   Plasmid

   #22185

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2001 Mar 16. 276(11):8377-8

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Deleted amino acids 301-306

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pEBG-TFII-I-delta N90

   Plasmid

   #22165

   Depositor

   Ananda Roy

   Article

   Sacristan et al J Biol Chem. 2004 Feb 20. 279(8):7147-58

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Tags

   GST and His

   Expression

   Mammalian

   Mutation

   The first 90 aa of TFII-I are deleted

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pEBB-II-I-EGFP delta isoform

   Plasmid

   #22149

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEBG-TFII-I N90

   Plasmid

   #22186

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2001 Mar 16. 276(11):8377-8

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Contains the first 90 aa of TFII-I

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEBB-II-I-deltaNLS1-EGFP

   Plasmid

   #22150

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 297-304

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEBG-TFII-deltaNLS1

   Plasmid

   #22153

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Tags

   GST and His

   Expression

   Mammalian

   Mutation

   deletion of amino acids 297-304

   Promoter

   Available since

   Nov. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 300_pETcon_SARS2_FLip

    Plasmid

    #222230

    Purpose

    yeast surface display of the SARS-CoV-2 FLip variant RBD

    Depositor

    Tyler Starr

    Article

    Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

    Insert

    SARS-CoV-2 FLip RBD (S Budding Yeast, SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 297_pETcon_SARS2_Omicron-BA286

    Plasmid

    #222231

    Purpose

    yeast surface display of the SARS-CoV-2 BA.2.86 variant RBD

    Depositor

    Tyler Starr

    Article

    Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

    Insert

    SARS-CoV-2 BA.2.86 RBD (S Budding Yeast, SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 299_pETcon_SARS2_EG-5

    Plasmid

    #222232

    Purpose

    yeast surface display of the SARS-CoV-2 EG.5 variant RBD

    Depositor

    Tyler Starr

    Article

    Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

    Insert

    SARS-CoV-2 EG.5 RBD (S Budding Yeast, SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET_His6-MBP-TEV-Cas6-RT-Cas1_WT

    Plasmid

    #186274

    Purpose

    Expresses Thiomicrospira Cas6-RT-Cas1 in E. coli

    Depositor

    Jennifer Doudna

    Article

    Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

    Insert

    Thiomicropira Cas6-RT-Cas1
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. as-mOPN-PB0

    Plasmid

    #106450

    Purpose

    Bacterial expression of antisense to murine osteopontin

    Depositor

    Georg Weber

    Article

    Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

    Insert

    antisense-osteopontin (Spp1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    antisense

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TAL3527

    Plasmid

    #47246

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-retinitispigmentosa2(X-linkedrecessive)-right (rp2 Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

16. TAL3526

    Plasmid

    #47245

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-retinitispigmentosa2(X-linkedrecessive)-left (rp2 Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pcI-ts,ind+-(NSP8)

    Plasmid

    #160656

    Purpose

    Expresses codon optimized SARS-CoV-2 NSP8 in E. coli

    Depositor

    Kenneth Johnson

    Article

    Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

    Insert

    SARS-CoV-2 NSP8 (ORF1ab Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. WN10151

    Plasmid

    #80441

    Purpose

    Expresses human codon-optimized dead (832A) LbCpf1

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Insert

    dead (D832A) LbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21b-RBD-JA1

    Plasmid

    #167126

    Purpose

    Expresses SARS-CoV-2 RBD in bacterial system

    Depositor

    David Margulies

    Article

    Ahmad et al bioRxiv. 2021 Jan 27. doi: 10.1101/2021.

    Insert

    SARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    residues (333 to 529 aa)

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMX-Flag-WIP

    Plasmid

    #11772

    Depositor

    Jack Strominger

    Article

    Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

    Insert

    WIP (WIPF1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

21. pFUGW-ZipACR-EYFP

    Plasmid

    #88844

    Purpose

    Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    ZipACR-NotI-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MAC-SARS-CoV-2 S

    Plasmid

    #158371

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 S (S )
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pFUGW-RapACR-EYFP

    Plasmid

    #111075

    Purpose

    Fast anion channelrhodopsin (current half-decay time 3-10 ms depending on voltage and light intensity) for time-resolved inhibition of neuronal spiking

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin RapACR
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFUGW-RapACR_T111C-EYFP

    Plasmid

    #119195

    Purpose

    Fast anion channelrhodopsin mutant RapACR_T111C for time-resolved inhibition of neuronal spiking

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin RapACR fast mutant T111C
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mutation

    changed threonine 111 to cysteine

    Promoter

    Ubiquitin

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-myc-ASPM MTB

    Plasmid

    #27668

    Depositor

    Joseph Loturco

    Article

    Paramasivam et al Cell Cycle. 2007 Jul 1. 6(13):1605-12.

    Insert

    Abnormal spindle-like microcephaly associated (Aspm Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Contains amino‑terminal putative microtubule bind…

    Promoter

    Available since

    April 1, 2011

    Availability

    Academic Institutions and Nonprofits only

26. pRPL28(K55M) SpHIS5 (pNTI666)

    Plasmid

    #115436

    Purpose

    Expresses K55M RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K55M; MfeI site in intron

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSFG-TFII-I-GFP beta

    Plasmid

    #22200

    Depositor

    Ananda Roy

    Article

    Hakre et al Mol Cell. 2006 Oct 20. 24(2):301-8.

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-myc-ASPM CTR

    Plasmid

    #27669

    Depositor

    Joseph Loturco

    Article

    Paramasivam et al Cell Cycle. 2007 Jul 1. 6(13):1605-12.

    Insert

    Abnormal spindle-like microcephaly associated (Aspm Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Contains carboxy terminal region of ASPM (amino a…

    Promoter

    Available since

    March 21, 2011

    Availability

    Academic Institutions and Nonprofits only

29. pSFG-TFII-I-GFP delta

    Plasmid

    #22190

    Depositor

    Ananda Roy

    Article

    Desgranges et al Mol Cell Biol. 2005 Dec . 25(24):10940-5

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pISH-Emx2

    Plasmid

    #74346

    Purpose

    Use to synthesize riboprobes for in situ hybridization (ISH) probe for Emx2. Not for gene expression.

    Depositor

    Peter Mombaerts

    Article

    Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

    Insert

    empty spiracles homeobox 2 (Emx2 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBS U6 WIP RNAi

    Plasmid

    #11774

    Depositor

    Jack Strominger

    Article

    Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

    Insert

    WIP RNAi (WIPF1 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

32. pRSF1b-His8-TEV-PI31-1-151

    Plasmid

    #202593

    Purpose

    Bacterial expression of HsPI31-1-151

    Depositor

    Brenda Schulman

    Article

    Kraus et al EMBO Rep. 2023 Aug 3;24(8):e56399. doi:

    Insert

    PI31 (PSMF1 Human)
    

    Use

    Tags

    8xHis, TEV

    Expression

    Bacterial

    Mutation

    aa 1-151 only

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. 185_pETcon_SARS2_BQ1-1

    Plasmid

    #208085

    Purpose

    yeast surface display of the SARS-CoV-2 BQ.1.1 variant RBD

    Depositor

    Tyler Starr

    Article

    Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

    Insert

    SARS-CoV-2 BQ.1.1 RBD (S Budding Yeast, SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZ

    Plasmid

    #192379

    Purpose

    To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output (despite the presence of the lacZ endlinker).

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV-dLbCpf1-BE-EE

    Plasmid

    #154147

    Purpose

    Expresses dLbCpf1-BE-EE(dLbCas12a-BE-EE) in mammalian cells.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

    Insert

    dLbCpf1-BE-EE (dCas12a-BE-EE)
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    APOBEC1(R126E+R132E), dLbCpf1(D832A+E925A+D1148A)

    Promoter

    CMV

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRPL28 SpHIS5 (pNTI663)

    Plasmid

    #115433

    Purpose

    Expresses wild-type RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    MfeI site in intron

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRPL28(Q38P) SpHIS5 (pNTI664)

    Plasmid

    #115434

    Purpose

    Expresses Q38P RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Q38P; MfeI site in intron

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRPL28(H39L) SpHIS5 (pNTI665)

    Plasmid

    #115435

    Purpose

    Expresses H39L RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H39L; MfeI site in intron

    Promoter

    ashbya gospii TEF1 and endogenous

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEBG-TFII-I L30&37P

    Plasmid

    #22166

    Depositor

    Ananda Roy

    Article

    Sacristan et al J Biol Chem. 2004 Feb 20. 279(8):7147-58

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Tags

    GST and His

    Expression

    Mammalian

    Mutation

    Changed Leucine 30 to Proline and changed leucine…

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

40. pET30a-SARS-CoV2-NP

    Plasmid

    #165090

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pEGFP-C2_SEPT9 i4

    Plasmid

    #71617

    Purpose

    mammalian expression of human SEPT9 i4 with a GFP fusion

    Depositor

    Elias Spiliotis, Cristina Montagna

    Article

    Connolly et al Breast Cancer Res. 2011 Aug 10;13(4):R76

    Insert

    Septin 9 (SEPTIN9 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pDestTol2CG2-mnx1:ReaChR-citrine

    Plasmid

    #169046

    Purpose

    Destination transgenesis vector for expressing ReaChR fused with citrine in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

    Depositor

    Paola Arlotta

    Article

    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

    Insert

    ReaChR-Citrine
    

    Use

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    Promoter

    3xMnx1

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBbB8k-csg-nb112

    Plasmid

    #171702

    Purpose

    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the spike protein of SARS-CoV-2 via a flexible linker.

    Depositor

    Neel Joshi

    Article

    Birnbaum et al ACS ES T Water. 2022 Nov 11;2(11):1836-1

    Insert

    csgBACEFG, with nanobody fused to CsgA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pEGFP-C2_SEPT9 i1

    Plasmid

    #71609

    Purpose

    mammalian expression of human SEPT9 i1 with a GFP fusion.

    Depositor

    Elias Spiliotis, Cristina Montagna

    Article

    Connolly et al Breast Cancer Res. 2011 Aug 10;13(4):R76

    Insert

    Septin 9 (SEPTIN9 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pRC10-EFS-huLbCpf1-2A-Blast-WPRE

    Plasmid

    #123359

    Purpose

    Lentiviral vector to constitutively express humanized LbCpf1/Cas12a, with blasticidin resistance for selection.

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Methods. 2019 May;16(5):405-408. doi

    Insert

    human codon-optimized Cpf1/Cas12a from Lachnospiraceae bacterium,
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCMV-dLbCpf1-BE-YE1

    Plasmid

    #154145

    Purpose

    Expresses dLbCpf1-BE-YE1(dLbCas12a-BE-YE1) in mammalian cells.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

    Insert

    dLbCpf1-BE-YE1 (dCas12a-BE-YE1)
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    APOBEC1(W90Y+R126E), dLbCpf1(D832A+E925A+D1148A)

    Promoter

    CMV

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. PhyB-mCherry-Spc72

    Plasmid

    #51582

    Purpose

    Constitutively express spindle pole body localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Use

    Tags

    Spc72 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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48. pET30a-SARS-CoV2-ORF8

    Plasmid

    #165089

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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49. pBBK69 pHomL-RPL18Bp-SPC42-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179053

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of SPC42-Link-Scarlet(rfp), a marker for the spindle pole

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-SPC42-Link-Scarlet(rfp)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pMX-Flag-WIP 460 deletion

    Plasmid

    #11773

    Depositor

    Jack Strominger

    Article

    Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

    Insert

    WIP aa1-460 (WIPF1 Human)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    aa461-503 deleted

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only