Addgene

We narrowed to 18,950 results for: Cre

Showing: 5401 - 5450 of 18950 results

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pTK_Sox10_enh_84_Citrine

Plasmid

#130584

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox10_enh_84

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pN_35S/mEGFP

Plasmid

#132565

Purpose

mEGFP expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)

Depositor

Mathias Pribil

Article

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mEGFP

Use

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

Plasmid

#196710

Purpose

Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pPP378

Plasmid

#158797

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-1 (D371A)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRS315Nab3

Plasmid

#110532

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Article

Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

Insert

NAB3 (NAB3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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2xsspB-mSc-sspB-Tiam1

Plasmid

#114424

Purpose

Translocating Tiam1 to iLID and activate Rac1

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

Tiam1

Use

Tags

mScarlet

Expression

Mammalian

Mutation

truncated Tiam1 (64-437)

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-CaaX(tH)

Plasmid

#109539

Purpose

Multisite gateway vector for 3' tagging with the CaaX domain from Hras

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CaaX domain from Hras

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Wnt11 STOP

Plasmid

#35885

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

WNT11 (WNT11 Human)

Use

Entry vector

Tags

Expression

Mutation

None

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt3-V5

Plasmid

#35928

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3 (WNT3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pNH082

Plasmid

#42165

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-p19-T7-EGFP100

Plasmid

#46309

Depositor

Judy Lieberman

Article

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
EGFP100 DNA-1
linker
EGFP100 DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
iLID-eGFP-2xC2-iLID

Plasmid

#114418

Purpose

anchor iLID domain to plasma membrane with slower diffusion

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLBC0030

Plasmid

#172777

Purpose

Level 0 module containing NT2_nYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS N-terminal eYFP (1-174)

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0031

Plasmid

#172778

Purpose

Level 0 module containing CT_nYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS N-terminal eYFP (1-174)

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0040

Plasmid

#172779

Purpose

Level 0 module containing NT2_cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS C-terminal eYFP (175-238)

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0041

Plasmid

#172780

Purpose

Level 0 module containing CT_cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS C-terminal eYFP (175-238)

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pPAP002

Plasmid

#153489

Purpose

Level 1 episomal expression plasmid for P. pastoris

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pI4-MTU-DO-URA

Plasmid

#160025

Purpose

Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Uracil selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

Expression

Yeast

Mutation

Promoter

n/a

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMTU-DO-HIS

Plasmid

#160005

Purpose

Low-copy expression vector for K. marxianus, histidine selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

Expression

Yeast

Mutation

Promoter

n/a

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM9121_TwinStrep-GFP11

Plasmid

#153514

Purpose

TwinStrep-GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

TwinStrep-GFP11

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-SP-FKBP-mCherry-TM-V5

Plasmid

#186226

Purpose

mRNA synthesis of rapalog-induced cell adhesion molecule

Depositor

Yoshihiro Morishita

Article

Usami et al Genes Cells. 2022 Jun;27(6):436-450. doi

Insert

SP-FKBP-mCherry-TM-V5

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-p19-T7

Plasmid

#46306

Depositor

Judy Lieberman

Article

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
DNA-1 site
linker
DNA-2 site

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Mutation

Promoter

Tac

Available since

July 23, 2013

Availability

Academic Institutions and Nonprofits only
786 pCSmAKT2Acherry

Plasmid

#31167

Depositor

Nathan Lawson

Article

Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

Insert

Akt

Use

Expression vector

Tags

Myr and mCherry

Expression

Mutation

membrane tethered Akt

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pN_35S/mApple

Plasmid

#132566

Purpose

mApple expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)

Depositor

Mathias Pribil

Article

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mApple

Use

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pPP384

Plasmid

#158799

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-3 (D413A)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_Sox9_enh_345_Citrine

Plasmid

#130592

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox9_enh_345

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_Sox10_enh_10E2_Cherry

Plasmid

#130590

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox10_enh_10E2

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_Sox10_enh_87_Cerulean

Plasmid

#130586

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox10_enh_87

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_Sox10_enh_85_Citrine

Plasmid

#130585

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox10_enh_85

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGM9121_GFP11

Plasmid

#153508

Purpose

GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

GFP11

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pI2-MTU-DO-HIS

Plasmid

#160016

Purpose

Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Histidine selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

Expression

Yeast

Mutation

Promoter

n/a

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Wnt10A STOP

Plasmid

#35883

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt10A (WNT10A Human)

Use

Entry vector

Tags

Expression

Mutation

None

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBT0190

Plasmid

#172815

Purpose

Level 1 module containing OEP7-mTRQ_P4 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-OEP7-mTRQ-3U+TER35S

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBC0100

Plasmid

#172789

Purpose

Level 0 module containing CT_Stop

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Spacer module with stop codons.

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBC0150

Plasmid

#172795

Purpose

Level 0 module containing NT2_TurboID

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

TurboID with 3XHA tag

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBC0050

Plasmid

#172781

Purpose

Level 0 module containing NT2_cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS 3XFLAG tag N-terminal eYFP (1-174)

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBC0051

Plasmid

#172782

Purpose

Level 0 module containing CT_3XFLAGnYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS 3XFLAG tag N-terminal eYFP (1-174)

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK-flag-GFP 1-10 tetra Cys

Plasmid

#78589

Purpose

Expression flag-GFP 1-10 tetra Cys in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-GFP 1-10 tetra Cys

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
FR_luc

Plasmid

#31098

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

firefly luciferase

Use

Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pTK_Msx1_enh_264_Citrine

Plasmid

#130610

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Msx1_enh_264

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-EF1aMS2p65HSF1hyg-W

Plasmid

#112920

Purpose

Lentiviral vector expressing MS2p65HSF1 under the control of the long human EF1a promoter

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

MS2p65HSF1-T2Ahyg

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human EF1a promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
SPDK3214

Plasmid

#97406

Purpose

binary construct of ER-targeted GUS with sfCherry11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

ER-GUS-sfCherry11

Use

Tags

ER target

Expression

Plant

Mutation

Promoter

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMGT1

Plasmid

#109404

Purpose

Tetracycline resistance reporter of ChnR activation

Depositor

Jay Keasling

Article

Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

Insert

Tetracycline resistance reporter of ChnR activation

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFLAG-CMV4-ZIKV-NS4B

Plasmid

#178846

Purpose

Mammalian expression of ZIKV ORF

Depositor

Judy Lieberman

Article

Sen Santara et al Proc Natl Acad Sci U S A. 2021 Nov 23;11

Insert

ZIKV-NS4B

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA6-rbNHERF1

Plasmid

#32942

Depositor

Edward Weinman

Article

Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

Insert

SLC9A3R1 (SLC9A3R1 rabbit)

Use

Tags

T7 tag, his, and partial FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-8xHis

Plasmid

#101699

Purpose

8xHis in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

AATG-[8xHis]-AGCC

Use

Yeast expression

Tags

Expression

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CyclinB-GFP

Plasmid

#54465

Purpose

CyclinB-GFP in pIVT vector for in vitro transcription

Depositor

Michael Lampson

Article

Davydenko et al J Cell Biol. 2013 Jul 22;202(2):221-9. d

Insert

CyclinB

Use

Tags

GFP

Expression

Mammalian

Mutation

K67E and I150T mutations compared to GenBank refe…

Promoter

Available since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only
pI3-MTU-DO-HPH

Plasmid

#160207

Purpose

Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Hygromycin selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

Expression

Yeast

Mutation

Promoter

n/a

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pI3-MTU-DO-LEU

Plasmid

#160176

Purpose

Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Leucine selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

Expression

Yeast

Mutation

Promoter

n/a

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pI4-MTU-DO-LEU

Plasmid

#160029

Purpose

Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Leucine selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

Expression

Yeast

Mutation

Promoter

n/a

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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