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We narrowed to 22,079 results for: his

Showing: 5401 - 5450 of 22079 results

  1. pET15-His-∆NGlpG(C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G)

    Plasmid
    #155134
    Purpose
    Expresses N-terminally His-tagged E. coli GlpG C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G variant (residues 87-276) from pET15b
    Depositor
    Sinisa Urban
    Article
    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910
    Insert
    GlpG (glpG Escherichia coli)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    C104A/D243G/L244G/F245G/M247C/S248G/M249G/A250G, …
    Promoter
    T7
    Available since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    155134-plasmid-map-sequence-id-310516

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  2. pET-28b-T7-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1897)

    Plasmid
    #114074
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) with C-terminal NLS and His-tag
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RVR (S542R/K548V/N552R) with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    S542R, K548V and N552R
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114074-plasmid-map-sequence-id-220510

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  4. pET-28b-T7-henAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1935)

    Plasmid
    #114073
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a-HF1(E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, K548R and N282A
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114073-plasmid-map-sequence-id-220529

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  6. pET-28b-T7-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1931)

    Plasmid
    #114075
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, K548V and N552R
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114075-plasmid-map-sequence-id-220517

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  14. R620-M18-303: CMV51p> SARS-CoV-2 S-RBD(319-541)-His6 K417T/E484K/N501Y

    Plasmid
    #170202
    Purpose
    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant
    Depositor
    Dominic Esposito
    Article
    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.
    Insert
    SARS-CoV-2 RBD (S SARS-CoV-2)
    Use
    Tags
    His6
    Expression
    Mammalian
    Mutation
    K417T, E484K, N501Y
    Promoter
    CMV51
    Available since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170202-plasmid-map-sequence-id-334271

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  16. R620-M05-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 K417N/E484K/N501Y

    Plasmid
    #170191
    Purpose
    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant
    Depositor
    Dominic Esposito
    Article
    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.
    Insert
    SARS-CoV-2 RBD (S SARS-CoV-2)
    Use
    Tags
    His6
    Expression
    Mammalian
    Mutation
    K417N, E484K, N501Y
    Promoter
    CMV51
    Available since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170191-plasmid-map-sequence-id-334254

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  18. R620-M06-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP K417N/E484K/N501Y

    Plasmid
    #170192
    Purpose
    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant
    Depositor
    Dominic Esposito
    Article
    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.
    Insert
    SARS-CoV-2 RBD (S SARS-CoV-2)
    Use
    Tags
    3C-His8-SBP
    Expression
    Mammalian
    Mutation
    K417N, E484K, N501Y
    Promoter
    CMV51
    Available since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170192-plasmid-map-sequence-id-334252

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  19. R620-M19-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP K417T/E484K/N501Y

    Plasmid
    #170203
    Purpose
    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant
    Depositor
    Dominic Esposito
    Article
    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.
    Insert
    SARS-CoV-2 RBD (S SARS-CoV-2)
    Use
    Tags
    3C-His8-SBP
    Expression
    Mammalian
    Mutation
    K417T, E484K, N501Y
    Promoter
    CMV51
    Available since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170203-plasmid-map-sequence-id-334219

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  20. pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(HI)

    Plasmid
    #204699
    Purpose
    This plasmid allows for inducible expression of engineered BRD4-p300(HI) fusion tagged with mNeonGreen, in mammalian cells.
    Depositor
    Michael Rosen
    Article
    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1
    Insert
    BRD4-p300 (BRD4, EP300 Human)
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    deletion of all TF-bidning domains in p300
    Promoter
    CMV
    Available since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204699-plasmid-map-sequence-id-405091

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  21. pQE60 MGH

    Plasmid
    #204244
    Purpose
    Bacterial expression of a fusion of watermelon glyoxysomal malate dehydrogenase (MDH) and GFP with His tag; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MGH
    Use
    Tags
    6X His and GFP
    Expression
    Bacterial
    Mutation
    fusion of MDH and GFP with His tag
    Promoter
    Lac
    Available since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    204244-plasmid-map-sequence-id-411051

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  22. pAIP4_1JL1

    Plasmid
    #234206
    Purpose
    Heterologous protein expression of ribonuclease HI (RNase HI) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1JL1 (rnhA Escherichia coli)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234206-plasmid-map-sequence-id-464171

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  23. pcDNA3.1_FASN

    Plasmid
    #107138
    Purpose
    His tagged human FASN cloned in pcDNA3.1 vector for expression in mammalian cells
    Depositor
    Brijesh Kumar Singh
    Insert
    Fatty acid synthase (FASN Human)
    Use
    Tags
    His
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107138-plasmid-map-sequence-id-423159

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  24. pET-UBE3A iso 2 N terminus

    Plasmid
    #185643
    Purpose
    expression of His-tagged UBE3A isoform 2 1-87 in bacterial cells
    Depositor
    Irina Bezsonova
    Insert
    ubiquitin-protein ligase E3A (UBE3A Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    Sept. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    185643-plasmid-map-sequence-id-367329

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  25. pSPIH6

    Plasmid
    #190676
    Purpose
    E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)
    Depositor
    John Vederas
    Article
    Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi
    Inserts
    Small ubiquitin-like modifier
    Mxe GyrA intein with C-terminal chitin binding domain and His tag
    Use
    Tags
    Chitin binding domain, His tag, and N-terminal Hi…
    Expression
    Bacterial
    Mutation
    Added C-terminal his tag and None
    Promoter
    T7
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    190676-plasmid-map-sequence-id-383624

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  26. ScSpe1_pET20b(+)

    Plasmid
    #118343
    Purpose
    Expresses the His tagged Ornithine Decarboxylase from Saccharomyces cerevisiae in Escherichia coli BL21 or similar strains
    Depositor
    Markus Ralser
    Insert
    SPE1
    Use
    Tags
    Histidine
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    118343-plasmid-map-sequence-id-229413

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  27. pET24a MDH_AQUAR

    Plasmid
    #204239
    Purpose
    Bacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_AQUAR
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204239-plasmid-map-sequence-id-413339

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  28. pET24a MDH_9CREN

    Plasmid
    #204243
    Purpose
    Bacterial expression of I. islandicus malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_9CREN
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204243-plasmid-map-sequence-id-413343

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  29. pET24a MDH_STRAW

    Plasmid
    #204240
    Purpose
    Bacterial expression of S. avermitilis malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_STRAW (mdh Streptomyces avermitilis)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204240-plasmid-map-sequence-id-413348

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  30. pET24a MDH_CHLP8

    Plasmid
    #204242
    Purpose
    Bacterial expression of C. parvum (green sulfur photosynthetic bacterium) malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_CHLP8
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204242-plasmid-map-sequence-id-413340

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  31. pET28a MDHC_YEAST

    Plasmid
    #204232
    Purpose
    Bacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHC_YEAST (MDH2 Budding Yeast)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204232-plasmid-map-sequence-id-413337

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  32. pET24a MDHC1_ARATH

    Plasmid
    #204237
    Purpose
    Bacterial expression of Arabidopsis cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHC1_ARATH (AT1G04410 Mustard Weed)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204237-plasmid-map-sequence-id-413345

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  33. pET28a MDH_PFALCI

    Plasmid
    #204235
    Purpose
    Bacterial expression of unicellular protozoan parasite P. falciparum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_PFALCI (PF3D7_0618500 Plasmodium falciparum)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204235-plasmid-map-sequence-id-413338

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  34. pET24a MDH_HALMA

    Plasmid
    #204241
    Purpose
    Bacterial expression of H. marismortui (Dead Sea halophilic red archaeon) cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_HALMA (mdh Haloarcula marismortui)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204241-plasmid-map-sequence-id-413344

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  35. pET28a MDHM_YEAST

    Plasmid
    #204231
    Purpose
    Bacterial expression of yeast mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHM_YEAST (MDH1 Budding Yeast)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does not include transit peptide and includes a M…
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204231-plasmid-map-sequence-id-413335

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  36. pMT-Flag-RELISH

    Plasmid
    #63750
    Purpose
    Expresses drosophila RELISH tagged with a Flag epitopes, under control of metallothionein promoter
    Depositor
    Cyril Gueydan
    Insert
    Relish (Rel Fly)
    Use
    Tags
    Flag
    Expression
    Insect
    Mutation
    Promoter
    pMT
    Available since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    63750-plasmid-map-sequence-id-106428

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  37. pET28a MDHP_YEAST

    Plasmid
    #204233
    Purpose
    Bacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHP_YEAST (MDH3 Budding Yeast)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    The last 3 C-terminal residues (SKL) are the pero…
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204233-plasmid-map-sequence-id-413332

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  38. Ec dnaN-pHK/P

    Plasmid
    #237239
    Purpose
    over-express E. coli hk/P-dnaN (Pol III beta sliding clamp subunit, N-terminal cleveable his-kinase tag)
    Depositor
    Michael O'Donnell
    Insert
    dnaN (beta), N-terminal his-kinase cleavable tag (dnaN E. coli)
    Use
    Tags
    his-10+PKA kinase motif+PreScission Protease clea…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237239-plasmid-map-sequence-id-469256

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  39. pET24a MDHM1_ARATH

    Plasmid
    #204236
    Purpose
    Bacterial expression of Arabidopsis mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHM1_ARATH (mMDH1 Mustard Weed)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does done include the first 52 residues that are …
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204236-plasmid-map-sequence-id-413347

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  40. pET24a MDHNP_ARATH

    Plasmid
    #204238
    Purpose
    Bacterial expression of Arabidopsis chloroplastic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHNP_ARATH (AT5G58330 Mustard Weed)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does not include the chloroplastic transit sequen…
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204238-plasmid-map-sequence-id-413341

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  41. pET-30a(+)-N-SH2+IA

    Plasmid
    #111273
    Purpose
    express murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
    Use
    Tags
    HHHHHHENLYFQG
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111273-plasmid-map-sequence-id-215706

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  43. pET-Bst-LF-Idaho

    Plasmid
    #170469
    Purpose
    Expresses His-tagged Bst-LF-Idaho in E. coli
    Depositor
    Jonathan Schmid-Burgk
    Insert
    Bst-LF-Idaho
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170469-plasmid-map-sequence-id-338354

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  44. pJBL7068

    Plasmid
    #167237
    Purpose
    His-tagged sfGFP for protein purification
    Depositor
    Julius Lucks
    Insert
    pT7-6XHis-sfGFP-MGA
    Use
    Synthetic Biology
    Tags
    6X His
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167237-plasmid-map-sequence-id-331130

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  45. pRD421

    Plasmid
    #163112
    Purpose
    Production of His6_TEV-cleavable-linker_mEos3.2_H-NSdbd in BL21 (DE3) pLysS
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    His-tag_TEV-cleavable-linker_mEos3.2_H-NSdbd
    Use
    Tags
    His-tag with TEV-cleavable linker
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163112-plasmid-map-sequence-id-321831

    Enlarge

  47. pET22B_SA_PcrA

    Plasmid
    #102999
    Purpose
    The plasmid is used to express Staphylococcus aureus PcrA, a DNA helicase, with N-terminal His-tag and Gly-Gly linker
    Depositor
    Martin Webb
    Insert
    SA_PcrA
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    PcrA 199-2423
    Promoter
    T7
    Available since
    May 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    102999-plasmid-map-sequence-id-199646

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  48. pcDNA3-OPN-V5

    Plasmid
    #11617
    Depositor
    Steven Johnson
    Insert
    Osteopontin (SPP1 Human)
    Use
    Tags
    His and V5
    Expression
    Mammalian
    Mutation
    C9L and E160K
    Promoter
    CMV
    Available since
    July 18, 2007
    Availability
    Academic Institutions and Nonprofits only

  49. pColdII-SIRPT1-Loxodonta Africana

    Plasmid
    #75351
    Purpose
    Nt His-tag Loxodonta Africana 89-1368
    Depositor
    Kalle Gehring
    Insert
    Sacsin Loxodonta Africana 89-1368
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    75351-plasmid-map-sequence-id-142900

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  50. pANY8

    Plasmid
    #164900
    Purpose
    Containing His-ELP-intein multifunctional tag for protein purification by combining Ni-NTA-based and ELP-based strategy with a specially designed purification protocol.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-ELP-Intein-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    164900-plasmid-map-sequence-id-330483

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Showing: 5401 - 5450 of 22079 results