165,947 results

1. nuclear NAD+ biosensor

   Plasmid

   #186789

   Purpose

   NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleus

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   nuclear NAD+ biosensor
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lenti

   Plasmid

   #138315

   Purpose

   Lenti expression of mTgBFP2

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   mTAGBFP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. GLP1R-DuET

   Plasmid

   #213253

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GLP1R (GLP1R Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUK21

   Plasmid

   #49788

   Purpose

   E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

   Depositor

   Joachim Messing

   Article

   Vieira et al Gene. 1991 Apr;100:189-94.

   Type

   Empty backbone

   Use

   Cloning vector

   Promoter

   lacZ

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBabe-Kras Wt

   Plasmid

   #75282

   Purpose

   Expresses human Kras wild-type

   Depositor

   Channing Der

   Insert

   KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   None. Wild-Type

   Available Since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag p21 WT

   Plasmid

   #16240

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

   Insert

   p21 (CDKN1A Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. CLYBL-TO-hNGN2-BSD-mApple

   Plasmid

   #124229

   Purpose

   Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.

   Depositor

   Michael Ward

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   NGN2 (NEUROG2 Human)
   

   Expression

   Mammalian

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EF1a-IRES-Hygro

   Plasmid

   #85134

   Purpose

   lentiviral backbone

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_hU6-peg(ATP1A1-T804N)_mU6-BsmBI_Puro

   Plasmid

   #234070

   Purpose

   For lentiviral expression of user-specified pegRNA in tandem with epegRNA (ATP1A1-T804N) for ouabain co-selection

   Depositor

   Greg Findlay

   Article

   Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET29-bdSUMO-Anti-ALFA nanobody

    Plasmid

    #212311

    Purpose

    Expression of anti-ALFA nanobody

    Depositor

    Arthur Laganowsky

    Article

    Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

    Insert

    anti-ALFA nanobody
    

    Tags

    bdSUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. RMCE halo-flag DNA-PKcs

    Plasmid

    #233918

    Purpose

    Low copy number provides expression of human DNA-PKcs with an N-terminal halo-flag tag.

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    human DNA-PKcs (PRKDC Human)
    

    Tags

    flag-halo

    Expression

    Mammalian

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBABE-puro-hTERT-HA

    Plasmid

    #1772

    Depositor

    Bob Weinberg

    Article

    Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

    Insert

    hTERT (TERT Human)
    

    Use

    Retroviral

    Tags

    2XHA

    Expression

    Mammalian

    Available Since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQAT57

    Plasmid

    #223429

    Purpose

    T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-U6-epegRNA-SV40-puro

    Plasmid

    #240539

    Purpose

    Lentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

    Depositor

    Wensheng Wei

    Article

    Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

    Insert

    epegRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. GW1-pHRed

    Plasmid

    #31473

    Purpose

    genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima

    Depositor

    Gary Yellen

    Article

    Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

    Insert

    pHRed
    

    Expression

    Mammalian

    Available Since

    July 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid

    #185912

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpRY-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. RMCE K>R DNA-PKcs

    Plasmid

    #233917

    Purpose

    Low copy number provides expression of human DNA-PKcs with a single K>R substitution that ablates kinase activity.

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    human DNA-PKcs kinase inactive mutant K3753>R (PRKDC Human)
    

    Expression

    Mammalian

    Mutation

    K3753R

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA GFP10-ZIP

    Plasmid

    #182241

    Purpose

    Tripartite split-GFP mammalian. Express leucine zipper with GFP10

    Depositor

    Stéphanie Cabantous

    Article

    Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

    Insert

    GFP10-ZIP
    

    Tags

    split-GFP10 tripartite

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA ZIP-GFP11

    Plasmid

    #182242

    Purpose

    Tripartite split-GFP mammalian. Express leucine zipper with GFP11

    Depositor

    Stéphanie Cabantous

    Article

    Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

    Insert

    ZIP-GFP11
    

    Tags

    split-GFP11 tripartite

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA-FLAG-METTL3

    Plasmid

    #160250

    Purpose

    Express FLAG-METTL3 in human cells

    Depositor

    Alessandro Fatica

    Article

    Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

    Insert

    methyltransferase like 3 (METTL3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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