Addgene

We narrowed to 1,001 results for: Np

Showing: 401 - 440 of 1001 results

Results per page:

Sort results by:

ApoE3_mCh-SspB (pBS1144)

Plasmid

#185326

Purpose

For the mammalian expression of the human protein ApoE3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
UbG76V-eGFP-V5His_15aa

Plasmid

#23969

Depositor

Nico Dantuma

Article

Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

Insert

Ub-G76V-eGFP-V5HIS_15aa (UBB Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

G76V mutation in Ubiquitin. Ub fused to N-termin…

Promoter

Available since

April 16, 2010

Availability

Academic Institutions and Nonprofits only
Anti-Pan-Nav channel [K58/35R]

Plasmid

#128623

Purpose

Mammalian Expression Plasmid of anti-Pan-Nav channel. Derived from hybridoma K58/35.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-Nav Channel Pan recombinant mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Pan-Shank [N23B/49R]

Plasmid

#128637

Purpose

Mammalian Expression Plasmid of anti-Pan-Shank. Derived from hybridoma N23B/49.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-Shank Pan recombinant mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AfrLEA3m_mCh-2xFKBP (pBS1137)

Plasmid

#185321

Purpose

For the mammalian expression of the brine shrimp protein AfrLEA3m attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AfrLEA3m

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_APOE_HUMAN_E4_47-300 (pBS0847)

Plasmid

#185279

Purpose

For the mammalian expression of the human protein APOE_HUMAN_E4_47-300. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_E4_47-300

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_PvLEA4_repeats_k3_26 (pBS0762)

Plasmid

#185244

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k3_26

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3 Flag MFF iso5 WT

Plasmid

#74378

Purpose

Flag tagged human MFF isoform 5

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
Anti-S100A5 [N176A/35.1R]

Plasmid

#114560

Purpose

Mammalian Expression Plasmid of anti-S100A5 (Rat). Derived from hybridoma N176A/35.1.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-S100A5 (Rattus norvegicus) recombinant mouse monoclonal antibody (S100a5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Nrp1-AP-His

Plasmid

#71971

Purpose

Expresses the extracellular region of the Neuropilin 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp1 (Nrp1 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_SERF2_HUMAN (pBS0751)

Plasmid

#185239

Purpose

For the mammalian expression of the human protein SERF2_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

SERF2_HUMAN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CAHS2parrc-mCh-2xFKBP (pBS1109)

Plasmid

#185304

Purpose

For the mammalian expression of the tardigrade protein CAHS2parrc attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS2parrc

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_MAHS_RAMVA (pBS0310)

Plasmid

#185169

Purpose

For the mammalian expression of the tardigrade protein MAHS_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

MAHS_RAMVA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-TrpV3 [N15/4R]

Plasmid

#177501

Purpose

Mammalian Expression Plasmid of anti-TrpV3 (Rat). Derived from hybridoma N15/4.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-TrpV3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv3 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Pan-Ankyrin [N388A/60R]

Plasmid

#128640

Purpose

Mammalian Expression Plasmid of anti-Pan-Ankyrin. Derived from hybridoma N388A/60.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-Ankyrin Pan recombinant mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-VGlut3 [N34/34.2R]

Plasmid

#114494

Purpose

Mammalian Expression Plasmid of anti-VGlut3 (Rat). Derived from hybridoma N34/34.2.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-VGlut3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Slc17a8 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Pan-SAPAP [N127/31.1R]

Plasmid

#114529

Purpose

Mammalian Expression Plasmid of anti-Pan-SAPAP (Rat). Derived from hybridoma N127/31.1.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-SAPAP (Rattus norvegicus) recombinant mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-SVOP [N356/23R]

Plasmid

#114499

Purpose

Mammalian Expression Plasmid of anti-SVOP (Rat). Derived from hybridoma N356/23.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-SVOP (Rattus norvegicus) recombinant mouse monoclonal antibody (Svop Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Flag MFF iso1 WT

Plasmid

#74388

Purpose

Flag tagged human MFF isoform 1

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

delta exon 1 (1-26)

Promoter

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
ApoE2_mCh-SspB (pBS1142)

Plasmid

#185324

Purpose

For the mammalian expression of the human protein ApoE2 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_MAHS_RAMVA_81-236-FLAG (pBS0297)

Plasmid

#185328

Purpose

For the mammalian expression of the tardigrade protein MAHS_RAMVA_81-236-FLAG. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

MAHS_RAMVA_81-236-FLAG

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Flag MFF iso4 WT

Plasmid

#74390

Purpose

Flag tagged human MFF isoform 4

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
Anti-Pan-FHF-A [N235/22R]

Plasmid

#114509

Purpose

Mammalian Expression Plasmid of anti-Pan-FHF-A (Human). Derived from hybridoma N235/22.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-FHF-A (Homo sapiens) recombinant mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Pannexin-2 [N121A/31R]

Plasmid

#140073

Purpose

Mammalian Expression Plasmid of anti-Pannexin-2 (Rat). Derived from hybridoma N121A/31.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pannexin-2 (Rat) recombinant mouse monoclonal antibody (Panx2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-SNAT1 [N104/32.1R]

Plasmid

#114517

Purpose

Mammalian Expression Plasmid of anti-SNAT1 (Rat). Derived from hybridoma N104/32.1.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-SNAT1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Slc38a1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Flag MFF iso1 S172A

Plasmid

#74391

Purpose

Flag tagged human S172A MFF isoform 1 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

delta exon 1 (1-26) and Ser 172 Ala.

Promoter

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
USPQ-HTT-TALEN1

Plasmid

#92243

Purpose

TALEN targeting upstream of HTT gene (KKR), forms obligate heterodimer with USPQ-HTT-TALEN2

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

TALEN

Use

TALEN

Tags

Expression

Mutation

Promoter

CMV

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
USPQ-HTT-TALEN2

Plasmid

#92244

Purpose

TALEN targeting upstream of HTT gene (ELD), forms obligate heterodimer with USPQ-HTT-TALEN1

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

TALEN

Use

TALEN

Tags

Expression

Mutation

Promoter

CMV

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
gRNA[Sxl].1026B

Plasmid

#112688

Purpose

express gRNA targeting Sxl under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[Sxl] (Sxl Fly)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Synaptotagmin-10 [N269/73R]

Plasmid

#114558

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-10 (Mouse). Derived from hybridoma N269/73.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Synaptotagmin-10 (Mus musculus) recombinant mouse monoclonal antibody (Syt10 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Flag MFF iso4 S172A

Plasmid

#74393

Purpose

Flag tagged human S172A MFF isoform 4 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Ser 172 Ala (numbering based on MFF isoform 1)

Promoter

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
aTY178_pUAS_Dkk1-IRES-tagBFP2_WPRE

Plasmid

#225353

Purpose

Lentiviral vector for DKK1 and BFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

Dkk1 (Dkk1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
PKMC356_pTRE-ActivinA_IRES_BFP_WPRE

Plasmid

#225355

Purpose

Lentiviral vector for ActivinA and BFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

Actvin A and TagBFP (Inhba Mouse, Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
aTY135_mtk02_pCAG-nfGFPtether_P2A_H2B-IRFP_SV40pA

Plasmid

#225350

Purpose

piggyback backbone for non-fluorescent GFP tether and H2B-IRFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

H2B (H2B Human, Synthetic)

Use

Tags

miRFP and non-fluorescent GFP (Y67F)-P2A

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE323-LPIV Puro-P2A-miRFP720 ZF6x12-C-DsRed-Express2(x6) phEF1α-BlastR

Plasmid

#201539

Purpose

Landing pad integration vector used to generate ZF6 synTF reporter line (HIE156), assembled with pPD1178 (destination vector), pHIE324-329 (TUPV1-6), pHIE280 (TUPV7), pPD1157 (linker)

Depositor

Joshua Leonard

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Inserts

PuromycinR-P2A-miRFP720
ZF6x12-C-DsRed-Express2(x6)
BlasticidinR

Use

Tags

Expression

Mammalian

Mutation

Promoter

None, ZF6x12(C)_YB_TATA Minimal Promoter, and hEF…

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag

Plasmid

#191572

Purpose

Mutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.

Depositor

Joshua Leonard

Article

Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

Insert

SARS-CoV-2 Spike Protein (S Synthetic)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

D614G and F486S (confers reduced binding affinity…

Promoter

CMV

Available since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTFG011_Spike_Parental_D614G_3xFlag

Plasmid

#191571

Purpose

SARS-CoV-2 Spike protein under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. For expression and pseudotyping lentiviral particles.

Depositor

Joshua Leonard

Article

Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

Insert

SARS-CoV-2 Spike Protein (S Synthetic)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

D614G mutation. Last 19 amino acids truncated.

Promoter

CMV

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
95.b_pSTC0-zeo

Plasmid

#202335

Purpose

Design 95.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

95.b

Use

Tags

Expression

Mutation

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
90.b_pSTC0-zeo

Plasmid

#202337

Purpose

Design 90.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

90.b

Use

Tags

Expression

Mutation

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
80.b_pSTC0-zeo

Plasmid

#202339

Purpose

Design 80.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

80.b

Use

Tags

Expression

Mutation

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 401 - 440 of 1001 results

Results per page:

Sort results by: