We narrowed to 34,221 results for: cas

1. pEF_dCas9

   Plasmid

   #68416

   Purpose

   Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A/H841A

   Promoter

   human EF1[alpha]

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDY1462_SERPINA1_CCA32_Caspase

   Plasmid

   #193194

   Purpose

   Express Caspase conditional upon RNA transcript

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

   Insert

   iCaspase9 (CASP9 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-iCas9

   Plasmid

   #160048

   Purpose

   inducible expression of Cas9

   Depositor

   Xiaojun Lian

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpCas9-NG

   Plasmid

   #138566

   Purpose

   Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   SpCas9-NG
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTPGI_dCas9_VP64

   Plasmid

   #49013

   Purpose

   encodes yeast-optimized dCas9_VP64 synthetic transcription factor

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   pTPGI (galactose+aTc inducible)

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmiCas9

   Plasmid

   #162758

   Purpose

   express miCas9

   Depositor

   Eugene Chen

   Article

   Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

   Insert

   miCas9
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Mammalian

   Mutation

   Contains Brex27 sequence range is 4958-5065

   Promoter

   CMV

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. NAX_CAGGS_CAS9_m4_KRAB_mcherry

   Plasmid

   #167878

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

8. pSpCas9_human_ADAR1_ccC

   Plasmid

   #158115

   Purpose

   deletion hADAR1

   Depositor

   Emanuela Felley-Bosco

   Insert

   Adar1 (ADAR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9_human_ADAR1_ccB

   Plasmid

   #158116

   Purpose

   deletion hADAR1

   Depositor

   Emanuela Felley-Bosco

   Insert

   Adar1 (ADAR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin

    Plasmid

    #211471

    Purpose

    lentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistance

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    Cas9_T2A_mNeonGreen_P2A_BlasticidinR
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, EF-1a

    Available since

    March 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQdCas12a.sggfp(C)

    Plasmid

    #236042

    Purpose

    The plasmid pQdCas12a.sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene.

    Depositor

    Radhakrishnan Mahadevan

    Insert

    quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    quorum sensing promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBbdCas9S

    Plasmid

    #149638

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, empty

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3_dCas7-11

    Plasmid

    #197605

    Purpose

    Expresses inactive Cas7-11 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2022 Nov 25;378(6622):874-881.

    Insert

    dCas7-11
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas9-HE

    Plasmid

    #109400

    Purpose

    Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein

    Depositor

    Jean-Paul Concordet

    Article

    Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

    Insert

    HDR-Enhancer domain of human CtIP protein (RBBP8 Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. PtPuc3_diaCas9_sgRNA

    Plasmid

    #109219

    Purpose

    Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum

    Depositor

    Per Winge

    Article

    Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10

    Inserts

    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    LHCF1 terminator, LHCF2 promoter, U6 3' regi…

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. nLbCas12a

    Plasmid

    #213745

    Purpose

    For circular RNA-mediated prime editor using nLbCas12a-D156R-R1138A in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    nLbCas12a
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R, R1138A

    Promoter

    CMV

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. SaCas9

    Plasmid

    #188494

    Purpose

    Expresses FLAG tagged SaCas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNOC_hfnCas12a_CRATES

    Plasmid

    #176251

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and type IIS restriction site for endonuclease BaeI at the crRNA region that facilitates the generation of mulitpl

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. SaCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122090

    Purpose

    U6 driven SaCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SaCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. RCASBP-AP (A) (CC#37)

    Plasmid

    #15160

    Depositor

    Connie Cepko

    Article

    Fekete et al Mol Cell Biol. 1993 Apr . 13(4):2604-13.

    Insert

    PLAP (ALPP Human)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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