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Plasmid#127968Purposeconstitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCLYBL-CAG-dCas9-NLS-BFP-KRAB
UseTALENExpressionMammalianPromoterCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti6-DEST PINK1-V5 WT
Plasmid#13320DepositorAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSB700
Plasmid#64046PurposeLentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent proteinDepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyExpressionMammalianPromoterU6, CAGGSAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT7-PE7 for IVT
Plasmid#214813PurposeTemplate for in vitro transcription of PE7DepositorInsertPE7
UseTemplate for in vitro transcriptionTagsSV40 bpNLS and c-Myc NLSPromoterT7 (inactivated)Available sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-VPR-c- Myc_NLS-mCherry-WPRE
Plasmid#176269PurposeExpression of dCasMINI-V4-VPR-mCherry in mammalian cellsDepositorInsertdCasMINI-V4-VPR-mCherry
UseLentiviralExpressionMammalianPromoterPGKAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64
Plasmid#180263PurposeLentiviral expression vector for dCas9-VP64 in mammalian cellsDepositorInsertdCas9
UseCRISPR and LentiviralExpressionMammalianMutationNuclease dead Cas9PromoterSFFVAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pAAV-FLEX-tdTomato
Plasmid#28306PurposeCre-dependent AAV-mediated expression of tdTomato under the CAG promoterDepositorHas serviceAAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.S, AAV PHP.eB, and AAV RetrogradeInserttdTomato
UseAAV and Cre/Lox; Adeno-associated virusExpressionMammalianMutationN/APromoterCAGAvailable sinceSept. 7, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GRAB_ACh3.0
Plasmid#121922Purposeexpress the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0DepositorHas serviceAAV9InsertGPCR activation based ACh sensor GRAB-ACh3.0
UseAAVPromoterhSynAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Integrin-Beta1-N-18
Plasmid#55064PurposeLocalization: Integrin/Focal Adhesions, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIntegrin-Beta1 (ITGB1 Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE_puro
Plasmid#118692PurposeEmpty vector to express overexpression cDNADepositorTypeEmpty backboneUseLentiviralPromoterCMVAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiGuide-Hygro-eGFP
Plasmid#99375PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-eGFP
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
C1(1-29)-TurboID-V5_pLX304
Plasmid#107175Purposeexpresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vectorDepositorInsertTurboID (BirA mutant)
UseLentiviralMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Available sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro
Plasmid#188768PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX-TRE-dCas9-KRAB-MeCP2-BSD
Plasmid#140690PurposeInducible knockdown of gene expression in human cells for pooled scRNA-seq experimentsDepositorPublicationInsertCas9m4-KRAB-MeCP2
UseCRISPR and LentiviralExpressionMammalianAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD6
Plasmid#73915Purposetissue-specific multiplex gRNA plasmidDepositorInsert:tRNA:gRNA:tRNA:gRNA:tRNA
UseCRISPRExpressionInsectPromoterUAS-DSCPAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBOB-CAG-SARS-CoV2-Spike-HA
Plasmid#141347Purpose3rd Generation lentiviral vector expressing the codon-optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M PaoDepositorInsertSARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
UseLentiviralTagsHAMutationresynthesized with human codon optimization nucle…PromoterCAGAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSL1142 (pSPIN, pBBR1 backbone)
Plasmid#160730PurposeSingle-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TFORF3549
Plasmid#145025PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.DepositorInsertGFP
UseLentiviralExpressionMammalianPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SpyCatcher003-sfGFP
Plasmid#133449PurposeExpresses SpyCatcher003-superfolder GFP in bacterial cytoplasmDepositorInsertSpyCatcher003-sfGFP
TagsHis6 and TEV siteExpressionBacterialPromoterT5Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by