We narrowed to 9,259 results for: Coli

1. pST5832

   Plasmid

   #36256

   Depositor

   Carolyn Bertozzi, Jessica Seeliger

   Article

   Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

   Insert

   lacZ
   

   Use

   Synthetic Biology; Mycobacteria replicating vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Modified Hsp60

   Available since

   Nov. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b T7pCONS sfGFP

   Plasmid

   #154467

   Purpose

   pET15b with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Use

   Tags

   His-thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. RSV-betaGal

   Plasmid

   #24058

   Depositor

   Herbert Samuels, Frederick Stanley

   Article

   Flug et al J Biol Chem. 1987 May 5. 262(13):6373-82

   Insert

   LacZ (lacZ E. coli)
   

   Use

   Tags

   RSV promoter

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pMS2

   Plasmid

   #191123

   Purpose

   Transcription of 12xMS2 repeats from a tetracycline-inducible promoter

   Depositor

   Ariel Lindner

   Article

   Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

   Insert

   MS2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   tetracycline-inducible promoter

   Available since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMYO127TAG-SepT

   Plasmid

   #34625

   Depositor

   Dieter Söll, Jesse Rinehart

   Article

   Park et al Science. 2011 Aug 26;333(6046):1151-4.

   Insert

   Myoglobin-127TAG
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   codon-optimized + an amber stop codon was introdu…

   Promoter

   Ipp

   Available since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXylA-agrCA-I

   Plasmid

   #53437

   Purpose

   xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

   Depositor

   Cynthia Collins

   Article

   Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

   Insert

   agrCA locus
   

   Use

   Synthetic Biology

   Tags

   none

   Expression

   Bacterial

   Mutation

   "MVQTS" sequence added to the N-terminu…

   Promoter

   PxylA

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBEST-p15A-Pl-tetO1-deGFP-T500 (122p)

   Plasmid

   #70198

   Purpose

   Promoter panel

   Depositor

   Richard Murray

   Article

   Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

   Insert

   deGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRT801

   Plasmid

   #68112

   Purpose

   Integration vector derived from Streptomyces phage phiBT1. Apramycin resistance gene, oriT, ColE1 origin of replication.

   Depositor

   Maggie Smith

   Article

   Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

   Inserts

   aac3(IV)
   oriT
   attP
   int
   

   Use

   Streptomyces integrating vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET30b_T7Sav

   Plasmid

   #138588

   Purpose

   T7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 ori

   Depositor

   Markus Jeschek

   Article

   Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

   Insert

   T7-tagged streptavidin variant with native C-terminus
   

   Use

   Synthetic Biology

   Tags

   T7-tag

   Expression

   Bacterial

   Mutation

   Promoter

   PT7

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEIAV-SIN6.1 CBLacZ-LucWm1A

    Plasmid

    #44167

    Depositor

    John Olsen

    Article

    Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

    Inserts

    LacZ
    firefly luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    Promoter

    CB, CMV enhancer/chicken beta-actin promoter

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEINO5

    Plasmid

    #179489

    Purpose

    Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

    Depositor

    Bogumil Karas

    Article

    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

    Insert

    aacC1
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHCKan-INSyibp-Nef

    Plasmid

    #134593

    Purpose

    Low Phosphate inducible Nef

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    HIV Nef protein
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    yibD

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCO706

    Plasmid

    #139743

    Purpose

    Individual component of Caffeic Acid biosynthesis pathway, hpaC

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    hpaB
    

    Use

    Moclo level 0 component

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCO707

    Plasmid

    #139744

    Purpose

    Individual component of Caffeic Acid biosynthesis pathway, hpaC

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    hpaC
    

    Use

    Moclo level 0 component

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDrop

    Plasmid

    #184872

    Purpose

    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pAL3

    Plasmid

    #114673

    Purpose

    T7 vector having very low basal expression of target protein can clone and express almost any gene. Asymmetric ligation clones as many as 3 genes simultaneously for co-expression from the same mRNA.

    Depositor

    William Studier

    Article

    Studier et al Curr Protoc Mol Biol. 2018 Oct;124(1):e6

    Type

    Empty backbone

    Use

    Tags

    optional Ser-Gly-6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. DVK_AF

    Plasmid

    #66068

    Purpose

    MoClo Destination Vector: (based on pSB1K3) [A:LacZa:F]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. GFP E17TAG/Q157TAG

    Plasmid

    #68299

    Purpose

    E17/Q157 TAG GFP reporter

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    GFP E17TAG/Q157TAG
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNVLTv2-pvdIJD-Ptrc-base

    Plasmid

    #169243

    Purpose

    Suicide vector for integrating expression cassette in P. putida (IPTG induction)

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRYG

    Plasmid

    #113642

    Purpose

    Positive control: shows the ratio of GFP signal to RFP signal with a tyrosine residue in the linker that connects the two genes.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    mRFP1-linker-sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLBC0151

    Plasmid

    #172796

    Purpose

    Level 0 module containing CT_TurboID

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    TurboID with 3XHA tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    no

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLV-EF1a-MinD-IRES-Puro

    Plasmid

    #213114

    Purpose

    Expression of untagged MinD in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinD (minD Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBT005

    Plasmid

    #177323

    Purpose

    MCP-SoxS (R93A) expression

    Depositor

    James Carothers

    Article

    Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

    Insert

    SoxS
    

    Use

    Tags

    MCP (MS2 Coat Protein)

    Expression

    Mutation

    R93A

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET29b-ClbPpep-CHis

    Plasmid

    #48243

    Purpose

    First 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domain

    Depositor

    Emily Balskus

    Article

    Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

    Insert

    ClbP-pep
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    First 375 amino acids of the peptidase, which con…

    Promoter

    T7

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p15aC-1D-5

    Plasmid

    #107865

    Purpose

    Bacterial expression of PIS and PI4P5K

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Inserts

    PIS
    phosphatidylinositol 4-phosphate 5-kinase
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    Promoter

    proD and proD (in operon)

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMA2867

    Plasmid

    #25439

    Depositor

    Mikhail Alexeyev

    Article

    Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

    Insert

    mCherry-miR155-lacZalpha (Mir155hg Mouse, Discosoma-mouse-E. coli)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p005kanRFP

    Plasmid

    #58533

    Purpose

    RFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    Red fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5 between two lac operators

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRSET FLII12Pglu-600u

    Plasmid

    #13563

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16A Y12S-ECFP-D13R
    

    Use

    Tags

    Citrine

    Expression

    Bacterial

    Mutation

    F16A ECFP insertion in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSB3K3-IC15

    Plasmid

    #165408

    Purpose

    Bacteria gene circuit on medium copy backbone.

    Depositor

    Xiaojun Tian

    Article

    Zhang et al Nat Commun. 2021 Feb 8;12(1):853. doi: 1

    Insert

    IC15 gene circuit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTU2-a-RFP

    Plasmid

    #72958

    Purpose

    Level 2 Destination vector - Position A - Assembly of 2 TU's from Level 1-AB

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - a site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only