We narrowed to 13,644 results for: NTS

1. FLAG-ZnT1-WT

   Plasmid

   #82340

   Purpose

   Expresses FLAG-tagged ZnT1-WT in mammalian cells

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

   Insert

   ZnT1-WT (SLC30A1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDGE332

   Plasmid

   #153241

   Purpose

   M1 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDGE333

   Plasmid

   #153242

   Purpose

   M2 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9

   Plasmid

   #175547

   Purpose

   Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag7 and Tomm20-HaloTag9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-PEmax-SpG-P2A-hMLH1dn

   Plasmid

   #211550

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

   Insert

   PEmax-SpG-P2A-hMLH1dn
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHACK-GAL4>QF2(newHA1)

   Plasmid

   #104873

   Purpose

   HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

   Depositor

   Christopher Potter

   Article

   Lin et al Genetics. 2016 Jun 22. pii: genetics.116

   Insert

   T2A-QF2
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDGE335

   Plasmid

   #153244

   Purpose

   M3 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LentiV2-mU6-evopreQ1

   Plasmid

   #210190

   Purpose

   pegRNA/ngRNA library cloning

   Depositor

   Yin Shen

   Article

   Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDR111_mannose_Bgal no NLS

   Plasmid

   #188399

   Purpose

   Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

   Depositor

   Christopher Contag

   Article

   Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

   Insert

   lacZ
   

   Use

   Tags

   PhoD secretion peptide

   Expression

   Bacterial

   Mutation

   Promoter

   D-mannose

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBJ2

    Plasmid

    #167131

    Purpose

    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Kanamycin promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. tetO-TBX5

    Plasmid

    #46032

    Depositor

    John Gearhart

    Article

    Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

    Insert

    TBX5 (TBX5 Human)
    

    Use

    Lentiviral

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFastBac_human-IL-18

    Plasmid

    #214338

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMOD_A3701

    Plasmid

    #91052

    Purpose

    Module A, Promoter: 35S, Gene: Csy4-P2A-dAtCas9-VPR, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-dAtCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    35S

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTRANS_253

    Plasmid

    #91125

    Purpose

    Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: WDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KillerOrange /pQE-30

    Plasmid

    #74748

    Purpose

    KillerOrange gene for bacterial expression

    Depositor

    Konstantin Lukyanov

    Article

    Sarkisyan et al PLoS One. 2015 Dec 17;10(12):e0145287. d

    Insert

    KillerOrange
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    KillerRed with substitutions G3C Y66W D113S N145S…

    Promoter

    T5

    Available since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBMN-FKBP DD(L106P)-UbK0G75S-sfGFP

    Plasmid

    #170157

    Purpose

    Expression for LIBRON constructs

    Depositor

    Thomas Wandless

    Article

    Miyamae et al Cell Chem Biol. 2020 Dec 17;27(12):1573-

    Insert

    FKBP DD (L106P)-UbK0G75S
    

    Use

    Retroviral

    Tags

    sfGFP

    Expression

    Mutation

    change all lysin residues of ubiquitin to Arg, G7…

    Promoter

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FSW-HRP-TM

    Plasmid

    #82540

    Purpose

    Lentiviral vector for expressing HRP-TM in neurons

    Depositor

    Alice Ting

    Article

    Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

    Insert

    HRP fused to TM domain of PDGFR
    

    Use

    Lentiviral

    Tags

    HRP

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMSP3545-dCas9Str

    Plasmid

    #153516

    Purpose

    Expresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backbone

    Depositor

    Kimberly Kline

    Article

    Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

    Insert

    dCas9Str
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    nisA

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LentiV2-EF1α-nCas9/RT

    Plasmid

    #210188

    Purpose

    Expression of nCas9/RT-P2A-Puro

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Insert

    nCas9/RT-P2A-Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTPH418b

    Plasmid

    #185725

    Purpose

    Directed evolution of Cas9 adenine base editors with altered PAM compatibility

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    gIII(N)-NpuN-NpuC-gIII(C)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTPH412

    Plasmid

    #185724

    Purpose

    Directed evolution of Cas9 adenine base editors with altered PAM compatibility

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-gp41-8N
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    ecTadA(8e) R26G

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAT15482_ABE8e

    Plasmid

    #174120

    Purpose

    Expresses ABE8e with an EF1a promoter

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    eABE
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p{CFD4-3xP3::DsRed}

    Plasmid

    #86864

    Purpose

    Expresses DsRed in eyes and provides a backbone for expressing two gRNAs. With attB integration site.

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. His6-TEV-mCerulean3-pBluescript

    Plasmid

    #166757

    Purpose

    For purification of mCerulean3 fluorophore

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    mCerulean3
    

    Use

    Tags

    6x Histidine tag, and TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGBT9-NF1-GRD

    Plasmid

    #19993

    Depositor

    Fuyuhiko Tamanoi

    Article

    Morcos et al Mol Cell Biol. 1996 May . 16(5):2496-503

    Insert

    Neurofibromatosis type I (NF1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. HisTEV-cDAXX

    Plasmid

    #197012

    Purpose

    E. coli expression vector

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    DAXX
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDGE655

    Plasmid

    #153253

    Purpose

    M1 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

    Plasmid

    #63168

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-V5"
    

    Use

    Tags

    N-myristoylation

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCBC-MT2T3

    Plasmid

    #50594

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T2, gRNA scaffold, TaU3t plus, U6-26p, T3
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Cas9 Null

    Plasmid

    #198727

    Purpose

    Expresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pG10-90

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRK1043

    Plasmid

    #8835

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    tobacco etch virus protease, catalytic domain
    

    Use

    Tags

    MBP and polyarginine

    Expression

    Bacterial

    Mutation

    Autolysis-resistant S219V mutant

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBJ4

    Plasmid

    #167133

    Purpose

    A kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Kanamycin promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRK5-HA-SZT2

    Plasmid

    #87035

    Purpose

    mammalian expression of SZT2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    SZT2 (SZT2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. ArcLight-A242

    Plasmid

    #36857

    Depositor

    Vincent Pieribone

    Article

    Jin et al Neuron. 2012 Sep 6;75(5):779-85.

    Insert

    fluorescent protein voltage sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    semian CMV

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3-HA-ERK2 WT

    Plasmid

    #8974

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PKG I alpha WT

    Plasmid

    #16390

    Depositor

    Bernard Weinstein

    Article

    Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

    Insert

    PKG1 alpha (PRKG1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMarVF1

    Plasmid

    #99579

    Purpose

    Minitransposon delivery vector pMarVF1 (suicide vector) in V. fischeri ES114

    Depositor

    Mark Mandel

    Article

    Brooks JF et al Proc Natl Acad Sci U S A. 2014 Dec 2;111

    Insert

    V. fischeri mariner transposon (with erythromycin-resistance (ErmR) cassette)
    

    Use

    Suicide vector in v. fischeri es114

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRU324

    Plasmid

    #167691

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::Cas9i
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. CAG-DIO-mRuby2

    Plasmid

    #104058

    Purpose

    AAV expression of Cre-dependent reporter mRuby2

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    mRuby2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Map3k1

    Plasmid

    #29581

    Depositor

    Dustin Maly

    Article

    Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

    Insert

    Map3k1 (MAP3K1 Human)
    

    Use

    Tags

    GST, HIS6, and TEV

    Expression

    Bacterial

    Mutation

    Constitutively Active. Contains catalytic domain …

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only