We narrowed to 30,827 results for: Eng
-
-
pVMG0173_Cq-Actin5c-Cas9
Plasmid#169345PurposeExpression of Cas9 in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0173_Cq-Actin5c-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ009808Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVMG0212_Cq-nanos-Cas9
Plasmid#169348PurposeExpression of Cas9 in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0212_Cq-nanos-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ011551Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVMG0213_Cq-vasa-Cas9
Plasmid#169347PurposeExpression of Cas9 in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0213_Cq-vasa-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ009286Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM mGata3
Plasmid#25796DepositorInsertGata3 (Gata3 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAC1-pCR8-dCas9VP48
Plasmid#48214PurposedCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A) fusion with VP48 activation domain
UseCRISPR; Gateway donorTagsHA Tag and VP48ExpressionMutationD10A;H840APromoterAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHDR-CDKN2A-Ex2-CMV-H2B-mOrange2
Plasmid#110735PurposeRecombination template for replacing CDKN2A Exon2 with Ef1alpha-H2B-mOrange2DepositorInsertH2B-mOrange2
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(D323A/D325A)
Plasmid#86592PurposeExpresses human full-length Akt1(D323A/D325A) mutantDepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutationD323A/D325A transforming mutationsPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
p53-(1-393)-E285A-N268D
Plasmid#35090DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains aa 1-393 - E285A-N268DPromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
p53-(1-393)-E285V-N268D
Plasmid#35088DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains aa 1-393 - E285V-N268DPromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
tdEos-VASP-5
Plasmid#57685PurposeLocalization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertVASP
UseTagstdEosExpressionMammalianMutationA209T in VASPPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pInducer21 Flag-Sesn2 419A/422A/426A
Plasmid#61870PurposeDox inducible lentivirial expression of Flag-Sesn 419A/422A/426A (mouse) mutantDepositorInsertSesn2 (Sesn2 Mouse)
UseTagsFlagExpressionMammalianMutation419A/422A/426APromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVEA3-delta7
Plasmid#234802PurposeMammalian expression of bovine arrestin-3 (deleted residues 176, 177, 178, 182-185)DepositorInsertArrestin-3 (ARR3 Bovine)
UseTagsVenusExpressionMammalianMutationdeleted residues 176, 177, 178, 182-185PromoterAvailable sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-BmNG
Plasmid#232204PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: BSD-3xTy-T2A-3xTy-mNeonGreenDepositorInsertmNeongreen
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-BmSc
Plasmid#232205PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: BSD-3xTy-T2A-3xTy-mScarletDepositorInsertmScarlet
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-CT-BmSc
Plasmid#232208PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-BSD-3xTyDepositorInsertmScarlet
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-PmSc
Plasmid#232548PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mScarletDepositorInsertmScarlet
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl-2-K22R
Plasmid#220293Purposemammalian expression of Bcl-2-K22R tagged with GFPDepositorInsertBcl2 (BCL2 Human)
UseTagsEmerald GFPExpressionMammalianMutationK22RPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl-2-G101V
Plasmid#220295Purposemammalian expression of Bcl-2-G101V tagged with GFPDepositorInsertBcl2 (BCL2 Human)
UseTagsEmerald GFPExpressionMammalianMutationG101VPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl-2-K17R
Plasmid#220292Purposemammalian expression of Bcl-2-K17R tagged with GFPDepositorInsertBcl2 (BCL2 Human)
UseTagsEmerald GFPExpressionMammalianMutationK17RPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl-2-ΔBH1
Plasmid#220297Purposemammalian expression of Bcl-2-ΔBH1 tagged with GFPDepositorInsertBcl2 (BCL2 Human)
UseTagsEmerald GFPExpressionMammalianMutationBH1 deletedPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl-2-F104C
Plasmid#220296Purposemammalian expression of Bcl-2-F104C tagged with GFPDepositorInsertBcl2 (BCL2 Human)
UseTagsEmerald GFPExpressionMammalianMutationF104CPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl-2-ΔBH3
Plasmid#220299Purposemammalian expression of Bcl-2-ΔBH3 tagged with GFPDepositorInsertBcl2 (BCL2 Human)
UseTagsEmerald GFPExpressionMammalianMutationBH3 deletedPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl-2-ΔBH2
Plasmid#220298Purposemammalian expression of Bcl-2-ΔBH2 tagged with GFPDepositorInsertBcl2 (BCL2 Human)
UseTagsEmerald GFPExpressionMammalianMutationBH2 deletedPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl-2-T132L
Plasmid#220303Purposemammalian expression of Bcl-2-T132L tagged with GFPDepositorInsertBcl2 (BCL2 Human)
UseTagsEmerald GFPExpressionMammalianMutationT132LPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl-2-A131L
Plasmid#220302Purposemammalian expression of Bcl-2-A131L tagged with GFPDepositorInsertBcl2 (BCL2 Human)
UseTagsEmerald GFPExpressionMammalianMutationA131LPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl-2-G128L
Plasmid#220301Purposemammalian expression of Bcl-2-G128L tagged with GFPDepositorInsertBcl2 (BCL2 Human)
UseTagsEmerald GFPExpressionMammalianMutationG128LPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl-2-ΔBH4
Plasmid#220300Purposemammalian expression of Bcl-2-ΔBH4 tagged with GFPDepositorInsertBcl2 (BCL2 Human)
UseTagsEmerald GFPExpressionMammalianMutationBH4 deletedPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GCaMP6f-WPRE
Plasmid#216275PurposeAAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of green fluorescent calcium indicator GCaMP6fDepositorInsertGCaMP6f
UseAAV and Cre/LoxTags6XHIS and XpressExpressionMutationPromoterhuman Synapsin 1 (hSyn)Available sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH4316
Plasmid#200171PurposePflp-18 LoxP EBFP (stop) LoxP twk-40(gf) GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40(gf) GFPDepositorInserttwk-40 (twk-40 Nematode)
UseTagsGFPExpressionWormMutationPromoterPflp-18Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH4018
Plasmid#200043PurposePrig-3 FRT let858 (stop) FRT zif-1 SL2 EBFP unc-54 3' UTR C.elegans AVA and other neurons expression of zif EBFPDepositorInsertzif-1 (zif-1 Nematode)
UseTagsEBFPExpressionWormMutationPromoterPrig-3Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-Ef1a-FAS-GCaMP6f-WPRE
Plasmid#141237PurposeAAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)DepositorInsertGCaMP6f
UseAAV and Cre/LoxTags6XHIS and XpressExpressionMutationPromoterHuman elongation factor-1 alpha (EF-1 alpha)Available sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLINE1ΔORF2-scFvFc
Plasmid#206021PurposeThe ORF2 sequence was removed from pLINE1-scFvFcDepositorInsertmouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF2 sequence
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLINE1ΔORF1-2-scFvFc
Plasmid#206022PurposeThe ORF1 and ORF2 sequences were removed from pLINE1-scFvFcDepositorInsertmouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF1 and ORF2 sequences
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-SHTN1S
Plasmid#209561PurposeMammalian expression of Shtn1 short isoformDepositorInsertShtn1 short isoform (4930506M07Rik Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-PBD-GFP-U6ac-rac2 guides
Plasmid#168251Purpose"label active Rac under Rac2 neutrophil-specific knockout background"DepositorInsertPBD GFP
UseZebrafish expressionTagsExpressionMutationPromoterLyzCAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-Utrophin-GFP-U6ac-Rac2 guides
Plasmid#168254Purpose"label stable F-actin under Rac2 neutrophil-specific knockout background"DepositorInsertUtrophin-GFP
UseZebrafish expressionTagsExpressionMutationPromoterLyzCAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
puc19_N_tag
Plasmid#186650PurposeEmpty donor plasmid.DepositorInsertN-terminal 3xFlag-3xHA Tag
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-PBD-GFP-U6ac-ctrl guides
Plasmid#168252Purpose"label active Rac; control for neutrophil-specific knockout"DepositorInsertPBD GFP
UseZebrafish expressionTagsExpressionMutationPromoterLyzCAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-Utrophin-GFP-U6ac-ctrl guides
Plasmid#168253Purpose"label stable F-actin; control for neutrophil-specific knockout"DepositorInsertUtrophin-GFP
UseZebrafish expressionTagsExpressionMutationPromoterLyzCAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Tmem209
Plasmid#175154PurposeLentiviral expression of V5-tagged mouse Tmem209DepositorInsertTmem209 (Tmem209 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Zdhhc6-V5
Plasmid#175146PurposeLentiviral expression of mouse Zdhhc6-V5DepositorInsertZdhhc6 (Zdhhc6 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Ptdss2-V5
Plasmid#175143PurposeLentiviral expression of mouse Ptdss2-V5DepositorInsertPtdss2 (Ptdss2 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-mRnf185
Plasmid#175126PurposeLentiviral expression of V5-tagged mouse Rnf185DepositorInsertRnf185 (Rnf185 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmem43-V5
Plasmid#175111PurposeLentiviral expression of mouse Tmem43-V5DepositorInsertTmem43 (Tmem43 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Myc-MBP
Plasmid#175103PurposeLentiviral expression of Myc-tagged MBPDepositorInsertMBP
UseLentiviralTagsMycExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-DGCR8(+6)
Plasmid#171541PurposeEncodes mutant human DGCR8 proteinDepositorInsertDGCR8 (DGCR8 Human)
UseTagsExpressionMammalianMutationa six amino acid_TRLTEG_insertion in the L1 regionPromoterAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
TMD18
Plasmid#163673PurposeExpresses a ST6GAL1 mutant with 18 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
UseTagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TMD19
Plasmid#163674PurposeExpresses a ST6GAL1 mutant with 19 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
UseTagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
PdnaK-IR2-IR2 GFP pZa
Plasmid#170083PurposeGFP expression under the control of E. coli dnaK promoter engineered with double IR2 HAIR motif from M. tuberculosisDepositorInsertGreen fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPdnaK-IR2-IR2Available sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only
