We narrowed to 9,259 results for: Coli

1. pCRISP_IS

   Plasmid

   #120425

   Purpose

   This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.

   Depositor

   Tamás Fehér

   Article

   Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

   Insert

   CRISPR spacers targeting IS1, IS5, IS3
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   constitutive

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. E17TAG GFP zeo resistance

   Plasmid

   #69118

   Purpose

   For E17TAG GFP reporter expression in non-zeocin resistant strains

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   E17TAG GFP
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   E17 to TAG

   Promoter

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

3. tet operator

   Plasmid

   #8901

   Depositor

   Lynda Chin

   Article

   Chin et al Nature. 1999 Jul 29. 400(6743):468-72.

   Insert

   tet operator
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pBbE1k-2

   Plasmid

   #59945

   Purpose

   dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human

   Depositor

   Taek Soon Lee

   Article

   Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

   Inserts

   dihydropterine reductase
   pterin-4a-carbinolamine dehydratase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   pTrc

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTU1-B-RFP

   Plasmid

   #72940

   Purpose

   Level 1 Destination vector - Position B

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 1 - B site
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Beta-lactamase - G717C (nucleotide) - silent muta…

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSET FLIPglu-3.2mDelta13

   Plasmid

   #13560

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

   Insert

   FLIPglu F16A S112A Delta13
   

   Use

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   F16A, S112A in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXM03

   Plasmid

   #121150

   Purpose

   This plamid was used for biosynthesis of Prenylated 4‑Hydroxybenzoate in E.coli

   Depositor

   Minjuan Xu

   Article

   He et al ACS Synth Biol. 2018 Sep 21;7(9):2094-21

   Insert

   XimB, IDS2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. SepOTSκ

   Plasmid

   #68291

   Purpose

   Phosphoserine orthogonal translation system

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SepRS/EF-Sep, 4xtRNA-Sep-A37
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBW-m4092686

   Plasmid

   #153895

   Purpose

   Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Nearly native encoding;inactivating mutations in …

   Promoter

   Promoter | t7 consensus from NEB, G is +1

   Available since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pRSET FLII275Pglu-4.6m

    Plasmid

    #13565

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16A G275S-ECFP-K276R
    

    Use

    Tags

    Citrine

    Expression

    Bacterial

    Mutation

    F16A, ECFP insert in mglB

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIIS18-SapI

    Plasmid

    #128599

    Purpose

    Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

    Depositor

    Tohru Suzuki

    Article

    Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIIS18-BsmBI

    Plasmid

    #128648

    Purpose

    Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

    Depositor

    Tohru Suzuki

    Article

    Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pY1

    Plasmid

    #50597

    Purpose

    Backbone: pRBS-01, Origin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5,

    Depositor

    Jay Keasling

    Article

    Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

    Type

    Empty backbone

    Use

    Synthetic Biology; Empty vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLacUV5

    Available since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pRSET FLIPglu-2uDelta13

    Plasmid

    #13561

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16F D152A Delta13
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    F16A, D152A in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLBC0150

    Plasmid

    #172795

    Purpose

    Level 0 module containing NT2_TurboID

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    TurboID with 3XHA tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    no

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHCKan-yibDp-GST

    Plasmid

    #134592

    Purpose

    Low Phosphate inducible GST

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    Glutathione S-transferase
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    yibD

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPJ173(JPUB_006777)

    Plasmid

    #83834

    Purpose

    Carries NEW operon 10 final

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Inserts

    Kuste3336
    Kuste3339
    Kuste3341
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    op10_3339_for, op10_3341_for, and op10_BsaI_3336_…

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPJ167(JPUB_006765)

    Plasmid

    #83831

    Purpose

    Carries NEW operon 3 final

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Inserts

    Kuste2803
    Kuste2804
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    op3_2804_for and op3_BsmBI_2803_for

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Vh-HA-DHFR

    Plasmid

    #20217

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    DHFR (folA E. coli)
    

    Use

    Tags

    HA and Vh

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pKB237

    Plasmid

    #170030

    Purpose

    Encodes LacI-controlled expression of surface-displayed CCL19 and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    CCL19 (CCL19 Escherichia coli, Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTU1-A-lacZ

    Plasmid

    #72935

    Purpose

    Level 1 Destination vector - Position A

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 1 - A site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Beta-lactamase - G717C (nucleotide) - silent muta…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSB74

    Plasmid

    #19591

    Depositor

    Jay Keasling

    Article

    Bang et al Appl Environ Microbiol. 2000 Sep . 66(9)

    Insert

    thiosulfate reductase (phsB S. enterica serovar Typhimurium LT2)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

23. pCx55.5-TrpR

    Plasmid

    #86373

    Purpose

    for zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherry

    Depositor

    Rachel Wong

    Article

    Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

    Insert

    TrpR
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    cx55.5

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDY4

    Plasmid

    #53589

    Purpose

    Produces an active Lactuca sativa (lettuce) lycopene epsilon-cyclase in E. coli. Use with pAC-LYC or pAC-LYCipi to produce epsilon-carotene in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Proc Natl Acad Sci U S A. 2001 Feb 27;98

    Insert

    lcyE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-clbB

    Plasmid

    #114155

    Purpose

    Expresses full-length ClbB

    Depositor

    Emily Balskus

    Article

    Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

    Insert

    clbB
    

    Use

    Tags

    6xHis-thrombin-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBEL2003

    Plasmid

    #139888

    Purpose

    Expression plasmid for LetAB with 5 ring (rings 12347) LetB (without TM region) mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; delta515-746)-6xHis

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBEL2000

    Plasmid

    #139885

    Purpose

    Expression plasmid for LetAB with 2 ring (rings17) LetB (without TM region) mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; delta161-747)-6xHis

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pXylA-agrCA-IV

    Plasmid

    #53438

    Purpose

    xylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureus

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    agrCA locus
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Bacterial

    Mutation

    "MVQTS" sequence added to the N-terminu…

    Promoter

    PxylA

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMMB206-CT61

    Plasmid

    #165410

    Purpose

    Bacteria gene circuit on low copy backbone.

    Depositor

    Xiaojun Tian

    Article

    Zhang et al Nat Commun. 2021 Feb 8;12(1):853. doi: 1

    Insert

    CT61 gene circuit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRSET FLII32Pglu-2.2m

    Plasmid

    #13566

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16A P32S-ECFP-D33R
    

    Use

    Tags

    6xHis and Citrine

    Expression

    Bacterial

    Mutation

    F16A, ECFP insert in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge