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Plasmid#220785PurposeExpresses N-terminal EGFP-LST2 in mammalian cellsDepositorInsertLST2 (ZFYVE28 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-PiGM-Iq(eGFP)
Plasmid#221602PurposeA recombinant AAV2 plasmid encoding the PiGM-Iq system with CRY2PHR, CIBN and EGFP as expression marker. hSynapsin promoter for panneuronal expression.DepositorInsertPiGM-Iq (eGFP)
UseAAVTagsExpressionMutationRGS2 1-53 truncationPromoterHuman SynapsinAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-ArgiNLS-EGFP
Plasmid#220594PurposeCre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein.DepositorInsertEGFP
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-mEGFP (FspAI) (JM49)
Plasmid#206436PurposeExpression in Schneider cells for imagingDepositorTypeEmpty backboneUseTagsmEGFPExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-PH-mEGFP (FspAI) (JM50)
Plasmid#206450PurposemEGFP expression in Schneider cells for imagingDepositorInsertmEGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Blasticidin XLone-eGFP
Plasmid#159754PurposeAAVS1 donor plasmid for targeted inducible eGFP expression in human cellsDepositorInsertall-in-one tet-on system
UseCRISPR and TALEN; Donor plasmid for targeted knoc…TagsExpressionMammalianMutationPromoterTRE3GS inducible promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PGK EGFP-RAB3A-T86E
Plasmid#206148PurposePGK EGFP-RAB3A-T86EDepositorInsertRAB3A (RAB3A Human)
UseTagsEGFPExpressionMammalianMutationT86EPromoterPGKAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
PGK EGFP-RAB3A-T86A
Plasmid#206149PurposePGK EGFP-RAB3A-T86ADepositorInsertRAB3A (RAB3A Human)
UseTagsEGFPExpressionMammalianMutationT86APromoterPGKAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/T0-eGFP NFATC2IP
Plasmid#221433PurposeMammalian expression vector of eGFP tagged NFATC2IPDepositorInsertNFATC2IP (NFATC2IP Human)
UseTagseGFPExpressionMammalianMutation1st Methionine of NFATC2IP deletedPromoterCMVAvailable sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAM-AAV-hIsl2-EGFP
Plasmid#153206PurposeHuman Isl2 (hIsl2) promoter-mediates gene expression in retinal neurons with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EGFP.Flag-EZH2-mt1
Plasmid#220244PurposeExpresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.DepositorInsertEZH2 mt 1 (EZH2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationR27A, R31A, F165APromoterEF-1αAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-KSR1-CA3 (N-KSR)
Plasmid#217755PurposeFluorescent reporter for glucosyl-ceramide (putative, mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseTagsEGFPExpressionMammalianMutationCA3 domain aa 317-400PromoterCMVAvailable sinceJune 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-Repo-Man-1-890
Plasmid#184272PurposeExpresses C-terminally truncated GFP-RepoManDepositorInsertcell division cycle associated 2 (CDCA2 Human)
UseTagsGFPExpressionMammalianMutationtruncated Repo-Man after aminoacid 893PromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKSR1-CA3-GS-EGFP (C-KSR-GS)
Plasmid#217757PurposeFluorescent reporter for ceramide (mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseTagsEGFPExpressionMammalianMutationCA3 domain aa 317-400 with GGSSGGGGA linkerPromoterCMVAvailable sinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5_NLS-Halo-eGFP-BASU
Plasmid#218449Purposeexpressed the NLS-Halo-eGFP-BASU fusion protein via stable Flp-In integrationDepositorInsertNLS-Halo-eGFP-BASU
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-Flag-Miwi-EGFP
Plasmid#212575PurposeLentiviral expression of Flag-tagged mouse Miwi in mammalian cellsDepositorInsertMiwi (Piwil1 Mouse)
UseLentiviralTags5x Flag and SNAP tagExpressionMammalianMutationPromoterSFFVAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYN-eGFP-oPRE
Plasmid#218681PurposeAAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.DepositorInserteGFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGW1-ExSYTE-P2A-EGFP
Plasmid#216257PurposeExSYTE (P2A) EGFPDepositorInsertExSYTE P2A EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGW1-ExSYTE-EGFP-DTE
Plasmid#216260PurposeExSYTE - EGFP fusion with 3'UTR of Rat Arc DTEDepositorInsertExSYTE EGFP - Rat DTE
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
F23-pAc5.1 EGFP Cup
Plasmid#206488PurposeCup expression for Schneider cell imagingDepositorInsertCup
UseTagsEGFPExpressionInsectMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGS96_EGFP(A206K)-I3-01 AAVS donor
Plasmid#211865PurposeI3-01 transgene integration at AAVS1 safe harbor locusDepositorInsertI3-01
UseCRISPRTagsExpressionMutationN/APromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-APOBEC1-YTH-EGFP
Plasmid#209322PurposeAAV packaging vector containing a APOBEC1-YTH expression, a P2A-EGFP expression cassette,.DepositorInsertAPOBEC1-YTH-HA
UseAAVTagsHAExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-APOBEC1-YTHmut-EGFP
Plasmid#209323PurposeAAV packaging vector containing a APOBEC1-YTHmut expression cassette, a P2A-EGFP expression cassette.DepositorInsertAPOBEC1--YTHmut-HA
UseAAVTagsHAExpressionMutationYTH domain lacks AA 385-409 comprising the m6A-bi…PromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EGFP-APOBEC1-YTH
Plasmid#209319PurposeAAV packaging vector containing a EGFP-P2A expression cassette, APOBEC1-YTH cassette.DepositorInsertAPOBEC1-YTH-HA
UseAAVTagsHAExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EGFP-APOBEC1-YTHmut
Plasmid#209320PurposeAAV packaging vector containing a EGFP-P2A expression cassette, APOBEC1-YTHmut cassette.DepositorInsertAPOBEC1-YTHmut-HA
UseAAVTagsHAExpressionMutationYTH domain lacks AA 385-409 comprising the m6A-bi…PromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-EGFP12-TTTA
Plasmid#215857PurposeThis plasmid encodes sgRNA that target EGFP with stretch sequenceDepositorInsertsgRNA-EGFP12 with TTTA stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 EGFP-LC3B HRD
Plasmid#207551PurposeHomologous recombination donor to integrate and expression cassette for eGFP-LC3B in the AAVS1 locus of human cellsDepositorInsertTET inducible EGFP-LC3B
UseTagsEGFPExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 EGFP-GABARAPL1 HRD
Plasmid#207552PurposeHomologous recombination donor to integrate and expression cassette for EGFP-GABRAPL1 in the AAVS1 locus of human cellsDepositorInsertTET inducible EGFP-GABARAPL1
UseTagsEGFPExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-EGFP12-WT(TTTT)
Plasmid#215856PurposeThis plasmid encodes sgRNA that target EGFP with stretch sequenceDepositorInsertsgRNA-EGFP12 with TTTT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ORP8-H514A-H515A
Plasmid#208360PurposeMammalian expression of fluorescent N-terminally tagged H514A-H515A mutant of ORP8DepositorInsertORP8 (OSBPL8 Human)
UseTagsEGFPExpressionMammalianMutationH514A-H515APromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psgRNA-GST-EGFP-GPIBY55
Plasmid#213720PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIBY55 under a CMV promoter.DepositorInsertEGFP
UseCRISPRTagsGSTExpressionMutationPromoterCMVAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
psgRNA-GST-EGFP-GPIDAF
Plasmid#213719PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF under a CMV promoter.DepositorInsertEGFP
UseCRISPRTagsGSTExpressionMutationPromoterCMVAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
psgRNA-GST-EGFP-GPICEAM7
Plasmid#213721PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 under a CMV promoter.DepositorInsertEGFP
UseCRISPRTagsGSTExpressionMutationPromoterCMVAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-TetON-mEGFP-MYOD1_AroLITE
Plasmid#215618PurposeFor integration of MYOD1 AroLITE T2A mEGFPDepositorInsertMYOD1 AroLITE (MYOD1 Human)
UseTagsT2A mEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-TetON-mEGFP-NGN2_AroLITE_A
Plasmid#215611PurposeFor integration of NGN2 AroLITE T2A mEGFPDepositorInsertNGN2 AroLITE (NEUROG2 Human)
UseTagsT2A mEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-TetON-mEGFP-NGN2_AroPERFECT
Plasmid#215612PurposeFor integration of NGN2 AroLITE T2A mEGFPDepositorInsertNGN2 AroLITE (NEUROG2 Human)
UseTagsT2A mEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-TetON-mEGFP-MYOD1_AroLITE-C
Plasmid#215619PurposeFor integration of MYOD1 AroLITE C T2A mEGFPDepositorInsertMYOD1 AroLITE C (MYOD1 Human)
UseTagsT2A mEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-TetON-mEGFP-MYOD1_WT
Plasmid#215617PurposeFor integration of MYOD1 WT T2A mEGFPDepositorInsertMYOD1 (MYOD1 Human)
UseTagsT2A mEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-TetON-mEGFP-HOXD4_WT
Plasmid#215626PurposeFor integration of HOXD4 WT mEGFPDepositorInsertHOXD4 (HOXD4 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-TetON-mEGFP-MYOD1_AroPERFECT-C
Plasmid#215621PurposeFor integration of MYOD1 AroPERFECT C T2A mEGFPDepositorInsertMYOD1 AroPERFECT C (MYOD1 Human)
UseTagsT2A mEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only