We narrowed to 35,392 results for: CaS;

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pCaSpeR-act5cB-QF#7m1

Plasmid

#46126

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7m1

Expression

Insect

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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ciCas9(F22)_pcDNA5

Plasmid

#100552

Purpose

Expresses ciCas9(F22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

ciCas9(F22)

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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V1-MESA-45F-M-dCas9

Plasmid

#84504

Purpose

MESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64

Depositor

Joshua Leonard

Article

Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

Insert

V2-MESA-35F-M-dCas9

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV dCas9-MC (eGFP)

Plasmid

#89931

Purpose

Expresses the dCas9-MC fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

Depositor

Carl Novina

Article

Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

Insert

dCas9-MC

Tags

Flag

Expression

Mammalian

Mutation

deactivated Cas9, fragment of M.SssI (residues 27…

Promoter

CMV

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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SpyCas9 PE2-3HA

Plasmid

#169853

Purpose

Mammalian Expression, SpyCas9 prime editor with 3HA-tag

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SpCas9-H840A-M-MLV-3HA

Tags

3XHA tag and bpSV40 NLS

Expression

Mammalian

Promoter

cmv

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-ArCas12a-2AeGFP

Plasmid

#123632

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

ArCas12a

Tags

3xHA tag

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-LpCas12a-2AeGFP

Plasmid

#123635

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

LpCas12a

Tags

3xHA tag

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huLb5Cas12a

Plasmid

#174684

Purpose

Expresses 6xHis-MBP tagged Lb5Cas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huLb5Cas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pX459V2.0-SpCas9-HF4

Plasmid

#108295

Purpose

pX459 V2.0 (Plasmid #62988) with the Y450A, N497A, R661A, Q695A, and Q926A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG_ATP1A1-G7

Plasmid

#173203

Purpose

Expresses the ATP1A1 G7 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 17. pX330-like plasmid.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G7 sgRNA + FLAGless enhanced specificity Cas9 (1.1)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-TD-HilA

Plasmid

#153330

Purpose

Triggers secretion of SptP120 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-HA4-7c12 Tandem Monobody

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-dHeFSpCas9

Plasmid

#92116

Purpose

Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive HeFSpCas9 with FLAG tag

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

Promoter

Cbh

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pBLO 9.2_bCas9-CP199

Plasmid

#121496

Purpose

TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Promoter

p15A

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huHkCas12a

Plasmid

#174683

Purpose

Expresses 6xHis-MBP tagged HkCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huHkCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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RCAS(A) mWnt5a (CT#539)

Plasmid

#13936

Depositor

Cliff Tabin

Article

Hartmann et al Development. 2000 Jul . 127(14):3141-59.

Insert

Wnt-5a (Wnt5a Mouse)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
A3F-Cas9n-UGI

Plasmid

#119138

Purpose

Base editor made from APOBEC3F

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3F-Cas9 nickase-UGI

Use

CRISPR

Mutation

None

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pX330 NC-FKBP-Cas9

Plasmid

#162725

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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A3C-Cas9n-UGI

Plasmid

#119136

Purpose

Base editor made from APOBEC3C

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3C-Cas9 nickase-UGI

Use

CRISPR

Mutation

None

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; T3_gRNA

Plasmid

#199335

Purpose

pDEL161; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type III sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 type III sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2

Plasmid

#107730

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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