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Showing: 2221 - 2240 of 15071 results

  1. pCMV-T7-SpCas9(ARGIMR)-P2A-EGFP (AHK147)

    Plasmid
    #223069
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with ARGIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(ARGIMR)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(ARGIMR)=D1135A, S1136R, G1218G, E1219I, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223069-plasmid-map-sequence-id-447645

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  2. pCMV-T7-SpCas9(ARTWGR)-P2A-EGFP (RAS2995)

    Plasmid
    #223070
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with ARTWGR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(ARTWGR)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(ARTWGR)=D1135A, S1136R, G1218T, E1219W, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223070-plasmid-map-sequence-id-447646

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  3. pCMV-T7-SpCas9(KRHWMR)-P2A-EGFP_(RAS3808)

    Plasmid
    #228251
    Purpose
    pCMV human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(KRHWMR)-P2A-EGFP
    Use
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(ARGIMR)=D1135K, S1136R, G1218H, E1219W, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    228251-plasmid-map-sequence-id-451023

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  4. pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEV

    Plasmid
    #74055
    Purpose
    retroviral expression plasmid for human NFATc2/C(1-686, RIT) (without C-terminal region, with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc2, isoform C (NFATC2 AS 1-686 (N terminus deleted), Human)
    Use
    Retroviral
    Tags
    AVI-TEV and FLAG
    Expression
    Mammalian
    Mutation
    silent mutation A1723C in the sequence of NM_1730…
    Promoter
    Available since
    June 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    74055-plasmid-map-sequence-id-142272

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  9. Monomer NK

    Plasmid
    #31178
    Depositor
    Feng Zhang
    Insert
    Monomer-NK
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 29, 2011
    Availability
    Academic Institutions and Nonprofits only

  10. pICSL50025

    Plasmid
    #174589
    Purpose
    Level 0 C-terminal tag module carrying 6xHIS uncleavable
    Depositor
    Mark Banfield
    Insert
    6xHIS uncleavable
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174589-plasmid-map-sequence-id-344730

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  11. pICSL30017

    Plasmid
    #174583
    Purpose
    Level 0 N-terminal tag module carrying 6xHIS uncleavable
    Depositor
    Mark Banfield
    Insert
    6xHIS uncleavable
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174583-plasmid-map-sequence-id-344729

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  12. pICSL30022

    Plasmid
    #174586
    Purpose
    Level 0 N-terminal tag module carrying Twin Streptavidin uncleavable
    Depositor
    Mark Banfield
    Insert
    Twin Streptavidin uncleavable
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174586-plasmid-map-sequence-id-344732

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  13. pICSL50027

    Plasmid
    #174591
    Purpose
    Level 0 C-terminal tag module carrying Twin Streptavidin uncleavable
    Depositor
    Mark Banfield
    Insert
    Twin Streptavidin uncleavable
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174591-plasmid-map-sequence-id-344734

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  14. pMal-Halo

    Plasmid
    #209349
    Purpose
    Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and a HRV 3C cleavable C-terminal HaloTag-StepTagII
    Depositor
    Lucia Strader
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    209349-plasmid-map-sequence-id-419545

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  15. pMal-mRuby3

    Plasmid
    #209348
    Purpose
    Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and a HRV 3C cleavable C-terminal mRuby3-StepTagII
    Depositor
    Lucia Strader
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    209348-plasmid-map-sequence-id-419546

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  16. pHS3

    Plasmid
    #81038
    Purpose
    HO endonuclease expression
    Depositor
    John McCusker
    Insert
    NATMX - HO endonuclease
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF
    Available since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  19. pOpen-TEV protease

    Plasmid
    #165509
    Purpose
    Used for site-specific cleavage of proteins. TEV cleaves the protein consensus sequence EXXYXQ^S/G
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    TEV protease
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165509-plasmid-map-sequence-id-338590

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  20. pET28-iLOVf

    Plasmid
    #63723
    Purpose
    E coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteins
    Depositor
    Brian Smith
    Type
    Empty backbone
    Use
    Tags
    TEE, iLOV, 6xHis, thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    63723-plasmid-map-sequence-id-110780

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Showing: 2221 - 2240 of 15071 results