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PX458_ZNF326_iso1_2

Plasmid

#128244

Purpose

Encodes gRNA for 3' target of human ZNF326_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF326_iso1 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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PX458_THRA_iso1_1

Plasmid

#128245

Purpose

Encodes gRNA for 3' target of human THRA_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

THRA_iso1 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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PX458_THRA_iso1_2

Plasmid

#128246

Purpose

Encodes gRNA for 3' target of human THRA_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

THRA_iso1 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_SIX4_iso1_1

Plasmid

#128247

Purpose

Encodes gRNA for 3' target of human SIX4_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

SIX4_iso1 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_SIX4_iso1_2

Plasmid

#128248

Purpose

Encodes gRNA for 3' target of human SIX4_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

SIX4_iso1 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_ZHX3_iso1_1

Plasmid

#128249

Purpose

Encodes gRNA for 3' target of human ZHX3_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZHX3_iso1 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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PX458_ZBTB49_iso1_1

Plasmid

#128250

Purpose

Encodes gRNA for 3' target of human ZBTB49_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB49_iso1 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_ZBTB49_iso1_2

Plasmid

#128251

Purpose

Encodes gRNA for 3' target of human ZBTB49_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB49_iso1 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PX330_SSX2B

Plasmid

#128252

Purpose

Encodes gRNA for 3' target of human SSX2B

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

SSX2B gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJA46

Plasmid

#59928

Purpose

gRNA for cleavage at rde-1(D801) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

rde-1(D801) gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pJA45

Plasmid

#59927

Purpose

gRNA for cleavage at rde-1(D718) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

rde-1(D718) gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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Lenti-Cas9-gRNA-GFP

Plasmid

#124770

Purpose

3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCW-Cas9

Plasmid

#50661

Purpose

Doxycycline-inducible lentiviral expression of SpCas9

Depositor

David Sabatini, Eric Lander

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Insert

humanized S. pyogenes Cas9

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Tet ON

Available since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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ph7SK-gRNA

Plasmid

#53189

Purpose

Expresses the S. pyogenes sgRNA from the 7SK promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

human 7SK

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pCW-Cas9-Blast

Plasmid

#83481

Purpose

Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA

Depositor

Mohan Babu

Inserts

SpCas9
Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Replaced puromycin N-acetyltransferase on the ori…

Promoter

Tight Tre and human phosphoglycerate kinase 1 pro…

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCpf1

Plasmid

#122185

Purpose

Bacterial genome editing plasmid with kanamycin resistance marker

Depositor

Cheng-Cai Zhang

Article

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCpf1-sp

Plasmid

#122186

Purpose

Bacterial genome editing plasmid with spectinomycin resistance marker

Depositor

Cheng-Cai Zhang

Article

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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p15A_SpyCas9_CmR

Plasmid

#119162

Purpose

Expresses S. pyogenes Cas9

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

Cas9 from S. pyogenes (NEWENTRY )

Use

Crispr

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEcgRNA

Plasmid

#166581

Purpose

CRISPR-Cas9-assisted genome editing in Escherichia coli

Depositor

Sheng Yang

Article

Li et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCasPA

Plasmid

#113347

Purpose

Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

Depositor

Quanjiang Ji

Article

Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge