4,133 results

1. pLV hUbC-dCas9 VP64-T2A-GFP

   Plasmid

   #53192

   Purpose

   Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized dead Cas9 VP64 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFRT/TO/HIS/FLAG/HA-HNRNPU

   Plasmid

   #38068

   Depositor

   Markus Landthaler

   Article

   Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

   Insert

   HNRNPU heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A) (HNRNPU Human)
   

   Use

   Tags

   HIS/FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMIG-w

   Plasmid

   #12282

   Purpose

   a bicistronic IRES-GFP containing vector which is a variant of pMIG that contains a posttranscriptional regulatory element from the woodchuck hepatitis virus

   Depositor

   Luk Parijs

   Article

   Refaeli et al J Exp Med. 2002 Oct 7. 196(7):999-1005.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pEGFPN1-human Dynamin 1aa

   Plasmid

   #22163

   Depositor

   Pietro De Camilli

   Article

   Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

   Insert

   human Dynamin 1aa (DNM1 Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   D744N

   Promoter

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ai6

   Plasmid

   #22798

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

   Insert

   CAG-Floxed ZsGreen
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPGKpuro

   Plasmid

   #11349

   Depositor

   Rudolf Jaenisch

   Article

   Tucker et al Genes Dev. 1996 Apr 15. 10(8):1008-20.

   Insert

   pgk-1 5' and 3' regions (Pgk1 Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOPH6

   Plasmid

   #40315

   Depositor

   Shaun Lott

   Article

   Loo et al Protein Expr Purif. 2002 Feb;24(1):90-8.

   Insert

   Peptide:N-glycosidase F
   

   Use

   Tags

   6xHis and OmpA3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-ATG5

   Plasmid

   #13095

   Depositor

   Roberta Gottlieb

   Article

   Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

   Insert

   ATG5 (Atg5 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRK5 beta1

   Plasmid

   #16042

   Depositor

   Filippo Giancotti

   Article

   Giancotti et al Cell. 1990 Mar 9. 60(5):849-59.

   Insert

   beta1 (ITGB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GFP-C1-CAMKIIbeta

    Plasmid

    #21227

    Depositor

    Tobias Meyer

    Article

    Shen et al Neuron. 1998 Sep . 21(3):593-606.

    Insert

    CAMKIIbeta (Camk2b Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Lab plasmid KS 0002.

    Promoter

    Available since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-EGxxFP-Cetn1

    Plasmid

    #50717

    Purpose

    Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Insert

    Cetn1 (Cetn1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GFP-rGBD

    Plasmid

    #26732

    Depositor

    William Bement

    Article

    Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

    Insert

    rhotekin (rtkn2 Mouse)
    

    Use

    Reporter for rho

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    RhoA binding domain of rhotekin

    Promoter

    Available since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-GFPd2

    Plasmid

    #14760

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    EGFPd2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCI-SEP_NR2A

    Plasmid

    #23997

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    NR2A (Grin2a Rat)
    

    Use

    Tags

    SEP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFPV-mCherry

    Plasmid

    #20956

    Depositor

    Olivia Steele-Mortimer

    Article

    Drecktrah et al Traffic. 2008 Dec . 9(12):2117-29.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pWZL Neo Myr Flag PKM2

    Plasmid

    #20585

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PKM2 (PKM Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-Ef1a-DIO ChETA-EYFP

    Plasmid

    #26968

    Purpose

    AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    hChR2(E123T/H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123T, H134R

    Promoter

    EF1alpha

    Available since

    Jan. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti CMV/TO RasV12 Puro (w119-1)

    Plasmid

    #22262

    Depositor

    Eric Campeau

    Insert

    HRAS G12V (HRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    G12V mutant

    Promoter

    Available since

    Dec. 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lyn11-targeted FRB (LDR)

    Plasmid

    #20147

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    Lyn-linker-FRB (LDR) (MTOR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLNCX chick src Y527F

    Plasmid

    #13660

    Depositor

    Joan Brugge

    Insert

    src Y527F (SRC Chicken)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Y527F. Constitutive active mutant.

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA5/FRT/TO GFP HSPA8

    Plasmid

    #19487

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPA8 (HSPA8 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSUMO1

    Plasmid

    #52258

    Purpose

    Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-1 (SUMO1 Human)
    UBE2l (UBE2I Human)
    SAE-2 (UBA2 )
    SAE-1 (SAE1 Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Integrin beta 2 - mYFP

    Plasmid

    #8638

    Depositor

    Timothy Springer

    Article

    Kim et al Science 2003 Sep 19;301(5640):1720-5.

    Insert

    Integrin beta 2 (ITGB2 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    stop codon of beta2 was replaced with AgeI restri…

    Promoter

    Available since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAS1NB c Rosella I

    Plasmid

    #71245

    Purpose

    a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.

    Depositor

    Mark Prescott

    Article

    Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

    Insert

    Rosella
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1

    Available since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCS2-UTX-F

    Plasmid

    #17438

    Purpose

    Expression of UTX in mammalian cells

    Depositor

    Kai Ge

    Article

    Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

    Insert

    UTX (KDM6A Human)
    

    Use

    Tags

    6xMyc and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-ChrimsonR-tdT

    Plasmid

    #59169

    Purpose

    Expression vector for ChrimsonR-tdTomato

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-tdTomato
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    K176R

    Promoter

    CAG

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FUW-tetO-lox-hSOX2

    Plasmid

    #20729

    Depositor

    Rudolf Jaenisch

    Article

    Soldner et al Cell. 2009 Mar 6. 136(5):964-77.

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-EGFP-Rac1(T17N)

    Plasmid

    #13721

    Depositor

    Klaus Hahn

    Article

    Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

    Insert

    EGFP-Rac1(T17N) (RAC1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGFP-N1-RACK1

    Plasmid

    #41088

    Depositor

    Anna Huttenlocher

    Article

    Cox et al Mol Biol Cell. 2003 Feb;14(2):658-69.

    Insert

    Receptor for activated C kinase (RACK1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CTV

    Plasmid

    #15912

    Depositor

    Klaus Rajewsky

    Article

    Thai et al Science. 2007 Apr 27. 316(5824):604-8.

    Insert

    CAG-STOP-eGFP-ROSA26TV
    

    Use

    Cre/Lox; /stop

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge