We narrowed to 16,109 results for: form
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Plasmid#192407PurposeTo act as the 0-input state of the A NIMPLY B gate.DepositorInsertB3RT-Act2-B3RT::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantAvailable SinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP739_L2_pV01_0I_B3RT-OCS-Rluc_lacZ
Plasmid#192383PurposeTo act as the off state for testing of the alt B3 recombinase.DepositorInsertAct2::B3RT-OCS-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP740_L2pV1_1I_B3RT-OCS-Rluc_TCTP-B3o_lacZ
Plasmid#192384PurposeTo act as the on state for testing of the alt B3 recombinase.DepositorInsertAct2::B3RT-OCS-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP716_L2sV3_NAND_1IF2_FRT_Rluc_FlpF2_C1_lacZ
Plasmid#192416PurposeTo act as a 1-input state of the split-NAND gate.DepositorInsertFRT-Act2-FRT::RlucRluc
UseSynthetic BiologyTagsPESTExpressionPlantAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP717_L2sV3_NAND_2I_FRT_Rluc_Flp-C1_lacZ
Plasmid#192417PurposeTo act as the 2-input state of the split-NAND gate.DepositorInsertFRT-Act2-FRT::RlucRluc
UseSynthetic BiologyTagsPESTExpressionPlantAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP715_L2sV3_NAND_1IF1_FRT_Rluc_FlpF1_C1-NLS_lacZ
Plasmid#192415PurposeTo act as a 1-input state of the split-NAND gate.DepositorInsertFRT-Act2-FRT::RlucRluc
UseSynthetic BiologyTagsPESTExpressionPlantAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP691_L2_2I_FRT-OCS_Rluc_Flp_C1
Plasmid#192414PurposeTo act as the 2-input state of the split-AND gate.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP764_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_1IB_lacZ
Plasmid#192408PurposeTo act as a 1-input state of the A NIMPLY B gate.DepositorInsertB3RT-Act2-B3RT::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
FnRPR9-10
Plasmid#182568PurposeHuman fibronectin fragment, type-III repeats 9-10, R1374A, P1376A, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 9-10
TagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1374A, P1376A, R1379AAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
FnRPR8-10
Plasmid#182570PurposeHuman fibronectin fragment, type-III repeats 8-10, R1374A, P1376A, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 8-10
TagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1374A, P1376A, R1379AAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Myo1b motor
Plasmid#187364PurposeExpresses rat myosin 1b isoform b motor domain labelled with EGFPDepositorAvailable SinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15 PemrR-gfp biosensor
Plasmid#185793PurposeThe pET15 PemrR-gfp biosensor is transcriptional fusion of GFP with the promoter of the emrRAB operon. This biosensor is responsive to monoaromatics, such as vanillin and syringaldehyde.DepositorInsertpEmrR-gfp
TagsEGFPExpressionBacterialPromoterPemrRAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Myo1b-Tail
Plasmid#187365PurposeExpresses rat myosin 1b isoform b Tail domain labelled with EGFPDepositorAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
L1-AA027
Plasmid#185752PurposeLevel 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a).DepositorInsertHygR2
UseSynthetic BiologyExpressionPlantAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
membrane-HsPLK1-dead
Plasmid#185572Purposeto see protein dynamicsDepositorInsertPLK1-dead (PLK1 Human)
UseIn vitro transcription (mrna synthesis)Tagsmembrane targeting sequence from Neuromodulin (ML…MutationK82R kinase-dead mutantAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
FAK C-term
Plasmid#186147PurposeFor expresseion of recombinant FAK c-terminal domain in E. coliDepositorAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-V5-Nxph1
Plasmid#184336PurposeExpression of V5-tagged mouse neurexophilin-1DepositorAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-9-35S-11
Plasmid#176881PurposeMoClo Level 0 plasmid, contains 35SDepositorInsert35S terminator
ExpressionPlantMutationWTAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC0x_gRNA_Pos5b_Ptet
Plasmid#179338PurposegRNA Position Vector 5b, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 camDepositorInsertPtet gRNA with sfGFP dropout
UseCRISPRAvailable SinceMarch 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMC0x_gRNA_Pos5a_Ptet
Plasmid#179337PurposegRNA Position Vector 5a, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 camDepositorInsertPtet gRNA with sfGFP dropout
UseCRISPRAvailable SinceMarch 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Rp51a_SUS1 5'ss_No_BP_pBP-pUC18
Plasmid#100990PurposeContains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence). Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutationDepositorInsertRP51A (RPS17A Budding Yeast)
ExpressionBacterial and YeastMutationGGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->…Available SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_P0526-sgRNArodA
Plasmid#149658Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30, P0526Available SinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-mEos2-APEX2
Plasmid#115563PurposeLifeact-mEos2-APEX2 fusion for PALM and CLEM. Parton lab clone IETDepositorInsertmEos2
TagsAPEX2 and LifeactExpressionMammalianAvailable SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-APEX2 (GC rich)
Plasmid#115550PurposeMultisite gateway vector for 3' tagging with GC-rich APEX2. Used to make 115563. Parton lab clone HHSDepositorInsertAPEX2 (GC rich)
UseMultisite gateway entry cloneAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pLyGo-Ec-2
Plasmid#163128PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding MalE signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab19
Plasmid#140888PurposeExpression of zebrafish GFP-tagged Rab19 in mammalian cells.DepositorAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(2)
Plasmid#164271PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
TagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(1)
Plasmid#164270PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
TagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab26 in pCSDEST2
Plasmid#140884PurposeExpression of zebrafish GFP-tagged Rab26 in mammalian cells.DepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab40b in pCSDEST2
Plasmid#140883PurposeExpression of zebrafish GFP-tagged Rab40b in mammalian cells.DepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab38a
Plasmid#140887PurposeExpression of zebrafish GFP-tagged Rab38a in mammalian cells.DepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti_stgRNA-Puro
Plasmid#164124PurposeBackbone for lentiviral stgRNA library construction (library 1 and 2)DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterhuman U6Available SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9H(-10TC)_arr2
Plasmid#149587Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterPflaB, PpQE30Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNAflaB
Plasmid#149561PurposeflaB gRNA expression in B. burgdorferiDepositorInsertsgRNAflaB
UseCRISPRExpressionBacterialPromoterPsynAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_PflaBS-sgRNAflaB
Plasmid#149643Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30, PflaBSAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_arr2
Plasmid#149582Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterPflaB, PpQE30Available SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNArodA
Plasmid#149569PurposerodA gRNA expression in B. burgdorferiDepositorInsertsgRNArodA
UseCRISPRExpressionBacterialPromoterPsynAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_P0826L-sgRNAflaB
Plasmid#149648Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30, P0826LAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-11a (GB1 WT - A34F/V39L)
Plasmid#136610PurposeExpresses GB1 WT double mutant (A34F/V39L)DepositorInsertGB1 WT double mutant (A34F/V39L)
Tags6x-HistagExpressionBacterialMutationMutation from GB1 WT: A34F - V39LPromoterT7 promoterAvailable SinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_Psyn-sgRNArodA
Plasmid#149655Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30, PsynAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_PresTS-sgRNAflaB
Plasmid#149644Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30, PresTSAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_PresTL-sgRNAflaB
Plasmid#149645Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30, PresTLAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_P0026-sgRNAflaB
Plasmid#149646Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30, P0026Available SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_P0826S-sgRNAflaB
Plasmid#149647Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30, P0826SAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_Psyn-sgRNAftsI
Plasmid#149649Purposeall-in-one CRISPRi vector for targeting B. burgdorferi ftsIDepositorInsertdCas9, lacI, sgRNAftsI
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30, PsynAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_P0526-sgRNAftsI
Plasmid#149650Purposeall-in-one CRISPRi vector for targeting B. burgdorferi ftsIDepositorInsertdCas9, lacI, sgRNAftsI
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30, P0526Available SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9G(-10TC)_arr2
Plasmid#149585Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterPflaB, PpQE30Available SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9B(-10TC)_arr2
Plasmid#149586Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterPflaB, PpQE30Available SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9B_arr2
Plasmid#149572Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneExpressionBacterialPromoterPflaB, PpQE30Available SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only