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Showing: 3391 - 3420 of 29307 results

  2. pMA_Int

    Plasmid
    #110096
    Purpose
    E. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Insert
    mycophage integrase
    Use
    Required for transformation of attb integrating v…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110096-plasmid-map-sequence-id-211299

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  4. AAV-CamK2-tTA

    Plasmid
    #210731
    Purpose
    AAV vector expressing tetracycline-controlled transactivator (tTA) under CamK2 promoter (for expressing tTA in glutamatergic neurons)
    Depositor
    Wei Xu
    Article
    Thompson et al Cell Rep Methods. 2023 Dec 18;3(12):1006
    Insert
    tetracycline-controlled transactivator (tTA)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CamK2
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    210731-plasmid-map-sequence-id-418042

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  7. PB-CT3G-ERP2-MG-IRES2-mNeonGreen

    Plasmid
    #175506
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    175506-plasmid-map-sequence-id-345872

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  13. pCAT024

    Plasmid
    #171636
    Purpose
    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-BFP
    mTagBFP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    171636-plasmid-map-sequence-id-337539

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  18. OmEF1aCas9P2ApuroSB

    Plasmid
    #165484
    Purpose
    Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells
    Depositor
    Dietmar Kueltz
    Article
    Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10
    Insert
    Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
    Use
    CRISPR; Transposon
    Tags
    Expression
    Mutation
    Promoter
    Oreochromis mossambicus EF1 alpha promoter (OmEF1…
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    165484-plasmid-map-sequence-id-330622

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  28. pCMV MN (eGFP)

    Plasmid
    #89932
    Purpose
    Expresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.
    Depositor
    Carl Novina
    Article
    Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.
    Insert
    MN
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    M.SssI residues 2-272 fragment
    Promoter
    CMV
    Available since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    89932-plasmid-map-sequence-id-177997

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  29. pPotef-cdc3CB16-NatR

    Plasmid
    #86465
    Purpose
    Expresses an N-terminal Cdc3-mCherry fusion under the control of the constitutive Potef promotor. 16boxB stem-loops are integrated into the 3'UTR to visualize the mRNA.
    Depositor
    Michael Feldbrügge
    Article
    Baumann et al EMBO Rep. 2014 Jan;15(1):94-102. doi: 10
    Insert
    Cdc3
    Use
    Ustilago maydis expression
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Potef
    Available since
    March 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    86465-plasmid-map-sequence-id-173986

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  30. N-BLa 1.2

    Plasmid
    #88999
    Purpose
    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.
    Depositor
    Dieter Langosch
    Article
    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103
    Insert
    N-BLa 1.2 fusion protein
    Use
    Tags
    Flag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    May 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    88999-plasmid-map-sequence-id-179971

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Showing: 3391 - 3420 of 29307 results