We narrowed to 6,450 results for: siae
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Plasmid#157656PurposeGFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequenceDepositorInsertGFP-gRNA
UseInsert storage (replicative in e. coli)TagsGFP-gRNAAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Plas-CRISPR_reporter
Plasmid#157658PurposeCRISPR reporter for mRNA quantification, integrative plasmid into NPR2 geneDepositorInsertdCAS9-VP64, mCherry, KanMX
UseInsert storage (replicative in e. coli)MutationN28D in mCherry- please see depositor comments be…Available SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-NHX1-HaloTagx2
Plasmid#177107PurposeExpresses Nhx1-HaloTagx2 in yeast cellsDepositorInsertNHX1 (NHX1 Budding Yeast)
ExpressionYeastAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAID6
Plasmid#136989PurposeYeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4DepositorInsertsdLbCpf1-VP
dSpCas9-RD1152
SaCas9
Csy4
ExpressionYeastPromoterENO2, TDH3, TEF1, and TPI1Available SinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-NHX1-iGFP
Plasmid#177108PurposeExpresses Nhx1-iGFP in yeast cellsDepositorInsertNHX1 (NHX1 Budding Yeast)
ExpressionYeastAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-EM7-Erv1p
Plasmid#202484PurposeExpresses sulfhydryl oxidase (Erv1p) constitutivelyDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGSHU
Plasmid#72244PurposeContains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoterDepositorInsertsKlURA3
hyg
I-SceI
ExpressionBacterialAvailable SinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-13Myc-His3MX6
Plasmid#41602DepositorInsertHis3
UseYeast genomic targetingTags13Myc, A. gossypii translation elongation factor …Available SinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
EMC1-2EGFP-ter-NAT
Plasmid#184763PurposeMarker for yeast endoplasmic reticulum (ER). Integration of 2xGFP tag at EMC1 C terminus. Uses antibiotic resistance marker natMX6.DepositorAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBD-pZ-FLAG
Plasmid#183148PurposeYeast-two-hybrid BD vector with pZ promoter (activated by ZPM)DepositorInsertGAL4 BD
UseYeast two-hybridTagsFLAGExpressionYeastAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACT-Lex
Plasmid#183149PurposeYeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)DepositorInsertGAL4 AD
UseYeat two-hybridTagsHA tagExpressionYeastAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSJ2039 (pRS316-NOP1pr-GFP1-10-SCS2TM)
Plasmid#86418PurposeONM/ER split GFP1-10DepositorInsertNOP1pr-GFP1-10-Scs2TM
TagsGFP1-10ExpressionYeastPromoterNOP1Available SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-Gal80ts
Plasmid#51619Purposevertebrate expression of full-length temperature-sensitive Gal80 with stopDepositorInsertGal80ts (GAL80 Budding Yeast)
UseVertebrate expressionMutationtemperature sensitive (G203V, G323R)*Available SinceMarch 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
IRE1-2EGFP-ter-kanMX4
Plasmid#184766PurposeIntegration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.DepositorAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
LT2_7_OxPro
Plasmid#177709PurposePlasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)DepositorInsertspTRX2_5xUAS-ymYPET-tCYC1
pTEFmut8-mCherry-tADH1
UseUsed as donor dna in genome integration after lin…ExpressionYeastAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYSD063
Plasmid#212916PurposeEncodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertmRuby2
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFvL106
Plasmid#64751PurposeRecombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, URA selectionDepositorInsertRITE cassette
UseRite cassettePromoternoneAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKAR1
Plasmid#61625PurposeFRET-based biosensor for monitoring JNK activityDepositorInsertJNKAR1
TagsCitrine and ECFPExpressionMammalianPromoterCMVAvailable SinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVB3011
Plasmid#113389PurposeGalactose-inducible L-BC gag: cure killerDepositorInsertL-BC Gag
ExpressionYeastMutationPlease see depositor commentsAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Nat
Plasmid#232097PurposeYeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selectionDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
M4754 KanMX::HIS3 Disruptor Converter
Plasmid#51684PurposeYeast marker swap plasmid for converting KanMX3 to HIS3DepositorAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
MmSphk1
Plasmid#118599PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in miceDepositorInsertMus musculus sphingosine kinase 1
TagsFLAGExpressionYeastPromoterGAL1, 10Available SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-Cerulean-VPS21
Plasmid#18842DepositorAvailable SinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
ESCRT-I
Plasmid#17634DepositorInsertExpressionBacterialAvailable SinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
HsSPHK1
Plasmid#118091PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)DepositorInsertHomo sapiens Sphingosine Kinase 1
TagsFLAGExpressionYeastPromoterGAL1, 10Available SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFSKm4-ATF1
Plasmid#221133PurposeFormaldehyde sensor based on the frmR repressor, which expresses yeast alcohol acetyl transferase (ATF1) upon detectionDepositorAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLifeAct-miRFP713
Plasmid#136562PurposeF-actin labeling with a near-infrared fluorescent protein miRFP713DepositorInsertLifeAct-miRFP713
TagsmiRFP713ExpressionMammalianPromoterCMVAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBPhsFlp2::PEST
Plasmid#63171PurposeDrosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock PromoterDepositorInserthsFlp2::PEST
ExpressionInsectAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDS257
Plasmid#34971PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.DepositorInsertMid2(SS/TM)-GFP-LOVpep
ExpressionYeastAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH847-gst-optsc
Plasmid#96908PurposePlasmid for yeast expression of GST encoded by preferred codonsDepositorArticleInsertyeast codon optimised glutathione-S transferase
Tags8xhistidineExpressionYeastPromoterTDH3Available SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTS2374-Tier1-PhCMV-Gal4_VP16
Plasmid#169582PurposeTier-1 vector encoding PhCMV-driven Gal4-VP16 transactivator (PhCMV-Gal4-VP16-pA).DepositorInsertGAL4-transactivator
ExpressionMammalianPromoterPhCMVAvailable SinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
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LT2_12_GlyRNA
Plasmid#177710PurposePlasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)DepositorInsertspTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1
UseUsed as donor dna in genome integration after lin…ExpressionYeastAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p415TEF cyto roGFP2-Grx2(yeast)
Plasmid#65005PurposeYeast expression plasmid for roGFP2-Grx2; yeast codon optimized roGFP; yeast Grx2DepositorInsertGRX2
TagsroGFP2ExpressionYeastAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
HYG-TET-OFF – ubiquitin – LEU – 4FLAG
Plasmid#193315PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.DepositorInsertUbiquitin - Leucine - 4FLAG N-terminal tagging
Tags4FLAGExpressionBacterialAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDSAR-Cre-F2A-I-SceI
Plasmid#62302PurposeAnopheles docking site transgenesis plasmid expressing Cre recombinase and I-SceI nuclease, vasa promoter, DsRed markerDepositorInsertCre recombinase, I-SceI nuclease
ExpressionInsectPromotervasaAvailable SinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTW073
Plasmid#64754PurposeRecombination-Induced Tag Exchange (RITE) Cassette, HA to a V5 epitope tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassettePromoternoneAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMH5
Plasmid#52531Purposeexpresses FLP recombinase in DrosophilaDepositorAvailable SinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPROEX Hsp104 (1-575)
Plasmid#1318DepositorInsertHsp104 (1-575) (HSP104 Budding Yeast)
Tags6X His + TEV cleavageExpressionBacterialMutationaa1-575 of Hsp104Available SinceJan. 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ABKAR(T/A)
Plasmid#140052PurposeNegative control mutant for ABKAR biosensor.DepositorInsertABKAR(T/A)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceNov. 21, 2020AvailabilityAcademic Institutions and Nonprofits only