We narrowed to 11,551 results for: NSI

1. pGEM-sgMUC4_dual-MTS-3x-Set I

   Plasmid

   #162762

   Purpose

   Separately expressing 3 unique sgMUC4_dual-MTS (SetI)

   Depositor

   Antony K Chen

   Article

   Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

   Insert

   sgMUC4_dual-MTS_1; sgMUC4_dual-MTS_2; sgMUC4_dual-MTS_3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Flag Snail 4SA

   Plasmid

   #16220

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

   Insert

   Snail (SNAI1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   4SA: S108A, S112A, S116A, S120A

   Promoter

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

3. Lsg-LRRK2-1

   Plasmid

   #199277

   Purpose

   nicking sgRNA to induce LRRK2 (c. 6055 G > A) mutation using PE3

   Depositor

   Ting Zhou

   Article

   Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

   Insert

   LRRK2 nick
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYFAC-CH1

   Plasmid

   #168977

   Purpose

   AMA1 based expression in filamentous fungi. pYFAC-pyrG carrying PalcA ; unique restriction site: PacI

   Depositor

   Yit Heng Chooi

   Article

   Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

   Type

   Empty backbone

   Use

   Synthetic Biology; Heterologous expression in fil…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJW4.02

   Plasmid

   #62867

   Purpose

   Bacterial expression of MBP-PGL-1

   Depositor

   Geraldine Seydoux

   Article

   Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

   Insert

   MBP-PGL-1
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pZH518

   Plasmid

   #102671

   Purpose

   Tetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRK5-mFzd10-1D4

   Plasmid

   #42272

   Depositor

   Jeremy Nathans, Chris Garcia

   Article

   Yu et al Development. 2012 Dec 1;139(23):4383-94.

   Insert

   Frizzled 10 (Fzd10 Mouse)
   

   Use

   Tags

   1D4

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 28, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pOAD

   Plasmid

   #196825

   Purpose

   Yeast two-hybrid ; Gal4 activation domain

   Depositor

   Stan Fields

   Article

   Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

   Type

   Empty backbone

   Use

   Tags

   Gal4 Activation domain

   Expression

   Yeast

   Mutation

   Promoter

   ADH

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFGAMS-mMaple3

   Plasmid

   #154912

   Purpose

   Expresses human PFAS-mMaple3 fusion in mammalian cells

   Depositor

   Stephen Benkovic

   Article

   Chan et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

   Insert

   PFAS (PFAS Human)
   

   Use

   Tags

   mMaple3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMZ-432

    Plasmid

    #195463

    Purpose

    Lentiviral Integratable, 4OHT/TMX- and GZV-controllable expression of synZiFTR: ZF3-p65

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pSFFV - SMASh - ZF3 - p65 - ERT2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMZ-431

    Plasmid

    #195462

    Purpose

    Lentiviral Integratable, 4OHT/TMX- and GZV-controllable expression of synZiFTR: ZF3-p65

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pSFFV - ZF3 - p65 - ERT2 - SMASh
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. wt1aCreERT2

    Plasmid

    #108464

    Purpose

    Used to generate a cryst:mCherry wt1a:CreERT2 zebrafish transgenic line.

    Depositor

    Nadia Mercader Huber

    Article

    Sanchez-Iranzo et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

    Insert

    wt1aCreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLentiGuide_GFP_sgEPAS1#1

    Plasmid

    #183119

    Purpose

    pLentiGuide modified to express GFP and an sgRNa targeting EPAS1

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgEPAS1-1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLentiGuide_GFP_sgEPAS1#2

    Plasmid

    #183120

    Purpose

    pLentiGuide modified to express GFP and an sgRNA targeting EPAS1

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    EPAS1-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. human b1 ecto-pIRES

    Plasmid

    #113387

    Purpose

    Express human beta 1 ectodomain with secretion peptide, purification tags, and C-terminal clasp

    Depositor

    Timothy Springer

    Article

    Li et al EMBO J. 2017 Mar 1;36(5):629-645. doi: 1

    Insert

    human beta 1 ectodomain
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCfB9337 (pgRNA_IV-1_NatMX)

    Plasmid

    #161590

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Insert

    guiding RNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX6p1 AO7RE

    Plasmid

    #137843

    Purpose

    GST tagged AO7 encoding aa 126-258 for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-roxSTOProx-LynEGFP

    Plasmid

    #133923

    Purpose

    Reporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Dre-based recombination

    Depositor

    Frank Bradke, Sebastián Dupraz

    Article

    Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

    Insert

    LynEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCLC-mEos2

    Plasmid

    #38010

    Depositor

    Xiaowei Zhuang

    Article

    Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Use

    Tags

    mEos2

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS2 GFP-GSK3-MAPK-E3mut

    Plasmid

    #29691

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    GFP glycogen synthase kinase 3 recognition site E3 ligase site mutated primed by MAPK
    

    Use

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    recognition sites only, and E3 ligase site mutated

    Promoter

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

21. pAID-CMV linearizing in pX330

    Plasmid

    #140609

    Purpose

    Crispr/Cas9 plasmid for linearization of pAID-CMV

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    sgRNA for pAID-EF1a plasmid digestion
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. psiCHECK-2-Zfp36-3'UTR

    Plasmid

    #61780

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Zfp36

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Zfp36 3'UTR
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

23. Prohibitin-bio-His

    Plasmid

    #50800

    Purpose

    Expresses enzymatically monobiotinylated full length Prohibitin

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    Prohibitin
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDZ645 pKAN 1x-mCherry-12xPP7 V4

    Plasmid

    #73173

    Purpose

    Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    1x-mCherry
    

    Use

    Tags

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LV anti-FITC-LaG16-Y186F/Y229A-2Xmutant-T2A-BFP

    Plasmid

    #194934

    Purpose

    LV construct encoding Y186F/Y229A double mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y186F/Y229A)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. EK0354 SFFV-mCherry-SG(Bdnf.s1L72)SG-EGFP (FLP-IN)

    Plasmid

    #191151

    Purpose

    Expression of a 72 bp long sensor for murine Bdnf 3' UTR in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:Bdnf.s1L72:EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBABE-puro-mTurquoise2-Nuf2

    Plasmid

    #80761

    Purpose

    Express Nuf2 N-terminally labeled with mTurquoise2

    Depositor

    Daniel Needleman

    Article

    Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

    Insert

    Nuf2 (NUF2 Human)
    

    Use

    Retroviral

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMC-Ura-Cen-lvl1

    Plasmid

    #176182

    Purpose

    preassembled yeast MoClo level-1 backbone with GFP dropout and URA3 marker

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MAC_N_RAB5A

    Plasmid

    #111692

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    RAB5A (RAB5A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pZH509

    Plasmid

    #102664

    Purpose

    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Dom34(full length)

    Plasmid

    #19098

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

32. pMC-6-AtSpCas9-9

    Plasmid

    #197778

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    AtSpCas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMC-1-AtU6-Y

    Plasmid

    #197779

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    AtU6 promoter for sgRNA assembly
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMC-1-OsU6-X

    Plasmid

    #197780

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    OsU6 promoter for sgRNA assembly
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMC-Z-SpCas9-gRNA-repeat-polyT-11

    Plasmid

    #197781

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    gRNA repeat for U6 sgRNA assembly
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJin-290

    Plasmid

    #125718

    Purpose

    ABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19 with CGG RNAP-C

    Depositor

    Bryan Dickinson

    Article

    Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

    Insert

    PCGG-gRNA GFP-off expression, FKBP-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-FRB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pVL23 - pVND7

    Plasmid

    #116019

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pVND7
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Ilmol

    Plasmid

    #120310

    Purpose

    A red-shifted GEVI consists of triple mutant ciona based voltage sensing domain with optimized linker and dTomato K159R.

    Depositor

    Bradley Baker

    Article

    Yi et al Sci Rep. 2018 Oct 12;8(1):15199. doi: 10

    Insert

    Ilmol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBS B2rescue A70D

    Plasmid

    #78658

    Purpose

    Generates B2 inserts suitable for rescue

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al PLoS Genet. 2012;8(8):e1002842. doi: 10.

    Insert

    B2rescue cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. mEmerald-C-Src-7

    Plasmid

    #54052

    Purpose

    Localization: Membrane/Vesicles, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

    Insert

    C-Src
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits