We narrowed to 15,575 results for: nol

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pCAG-RFP6 POD-nAb-WPRE

Plasmid

#244974

Purpose

Expresses a fusion protein of a nanobody against RFP and peroxidase (POD) in mammalian cells

Depositor

Hiroyuki Hioki

Article

Yamauchi et al Commun Biol. 2025 Jun 18;8(1):903. doi:

Insert

RFP6 POD-nAb

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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RSVpGL3 (RSV promoter-luciferase in pGL3-basic)

Plasmid

#40343

Depositor

Alexander Dent

Article

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Insert

RSV promoter

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

RSV

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA-SV40-mCherry

Plasmid

#240538

Purpose

Lentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with mCherry. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pMM764

Plasmid

#68901

Purpose

IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Mutation

L51P

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCI-neo-sOVA

Plasmid

#25098

Depositor

Maria Castro

Article

Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

Insert

soluble ovalbumin

Expression

Bacterial and Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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EasyFusion T2A-H2B-mCherry

Plasmid

#113089

Purpose

General donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-mCherry

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pQE30-His-NDK

Plasmid

#124136

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Nucleotide diphosphate kinase (ndk Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-M13-TEV-C-P2A-TdTomato

Plasmid

#92389

Purpose

expresses Cal-light M13-TEV-C component and TdTomato in mammalian cells

Depositor

Hyungbae Kwon

Article

Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

M13-TEV-C-P2A-TdTomato

Tags

TdTomato

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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EasyFusion T2A-H2B-GFP

Plasmid

#112851

Purpose

General donor vector with T2A-H2B-GFP coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-GFP

Use

Synthetic Biology

Promoter

No

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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TAL3194

Plasmid

#41368

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-tfec-Left (tfec Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2012

Availability

Academic Institutions and Nonprofits only
TAL3195

Plasmid

#41369

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-tfec-Right (tfec Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2012

Availability

Academic Institutions and Nonprofits only
FosB

Plasmid

#187908

Purpose

Expresses FosB in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

FosB (Fosb Mouse)

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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MLM3613

Plasmid

#42251

Purpose

Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

Streptococcus pyogenes Cas9

Use

CRISPR; Zebrafish expression

Promoter

CMV

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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4xNLS-pMJ915v2-sfGFP

Plasmid

#88921

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pIVT-Cas9-TMV

Plasmid

#238016

Purpose

for DNA-free gene knockout editing in rice and wheat or other plants

Depositor

Caixia Gao

Article

Qiu et al Plant Biotechnol J. 2025 Apr;23(4):1348-

Insert

SpCas9

Expression

Plant

Promoter

T7

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pQE30-His-AlaRS

Plasmid

#124103

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Alanyl-tRNA ligase (alaS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBB572

Plasmid

#27397

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

ibpA, ibpB (ibpB, ibpA E.coli)

Expression

Bacterial

Available Since

March 28, 2011

Availability

Academic Institutions and Nonprofits only

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pYHY2

Plasmid

#216108

Purpose

Constitutive expression of secretory BT0294 SP-NanoLuc via BfP1E6-RBS8 promoter-RBS pair in Bacteroides species.

Depositor

Shannon Sirk

Article

Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

Insert

NanoLuc

Use

Synthetic Biology; E. coli-bacteroides shuttle ve…

Tags

3xFLAG tag and BT0294 signal peptide

Expression

Bacterial

Promoter

BfP1E6 promoter

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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PX856

Plasmid

#62888

Purpose

C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa536-1368)

Use

CRISPR

Tags

FKBP12, NLS, NLS SV40, and VP64

Expression

Mammalian

Mutation

Asparagine 863 to Alanine (N863A)

Promoter

CBh

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-RhoBAST

Plasmid

#196341

Purpose

Overexpression of circular RhoBAST

Depositor

Murat Sunbul

Article

Sunbul et al Nat Biotechnol. 2021 Jun;39(6):686-690.

Insert

Tornado-RhoBAST

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBB535

Plasmid

#27392

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

dnaK, dnaJ (dnaJ, dnaK E. coli)

Expression

Bacterial

Available Since

March 28, 2011

Availability

Academic Institutions and Nonprofits only

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gCH128 (non-targeting crRNA)

Plasmid

#217339

Purpose

Non-targeting crRNA

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

crNT-3

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAG1186 Lyn11-ECFP-FIREmate

Plasmid

#205168

Purpose

Expresses Lyn11-ECFP-FIREmate in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

Lyn11-ECFP-FIREmate

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GAVPO

Plasmid

#159923

Purpose

GAVPO (VIVID transactivator) expressed under the CMV promoter in the pcDNA3.1 backbone. AKA 133

Depositor

Fei Chen

Article

Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

Insert

GAVPO

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-cOVA

Plasmid

#25097

Depositor

Maria Castro

Article

Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

Insert

cytoplasmic ovalbumin

Expression

Bacterial and Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pRN114

Plasmid

#127221

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, Wus, AtSTM

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a.Tat(48-57)-EGFP

Plasmid

#165040

Purpose

Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

Depositor

Dokyun Na

Article

Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

Insert

EGFP

Tags

6XHis and Tat(48-57)

Expression

Bacterial

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pORF2

Plasmid

#206026

Purpose

An expression vector for mouse LINE1 ORF2

Depositor

Masamichi Kamihira

Article

Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

Insert

ORF2 derived from mouse LINE1

Expression

Mammalian

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDONRPEX18Km-SceI-pheS

Plasmid

#111610

Purpose

Gateway compatible allelic exchange vector for Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Type

Empty backbone

Use

Allelic exchange in burkholderia sp.

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG1169 FIREmate-ECFP-Cb5

Plasmid

#205167

Purpose

Expresses FIREmate-ECFP-Cb5 in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

FIREmate-ECFP-Cb5

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
DL033:cFos::eGFP-iPAK4

Plasmid

#177883

Purpose

eGFP-iPAK4 fusion with cFos promoter, cloned into a lentivirus backbone

Depositor

Adam Cohen

Article

Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

iPAK4

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

cFos

Available Since

July 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGNW6

Plasmid

#122093

Purpose

suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-PLE-IRES-mCherry

Plasmid

#45766

Depositor

Luke Lavis

Article

Tian et al Proc Natl Acad Sci U S A. 2012 Mar 27;10

Insert

esterase

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG1247 H2B-ECFP-FIREmate

Plasmid

#205171

Purpose

Expresses H2B-ECFP-FIREmate in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

H2B-ECFP-FIREmate

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE60-EFTu-His

Plasmid

#124127

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Elongation factor Tu (tufB Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CMV-PEmax-P2A-EGFP

Plasmid

#240534

Purpose

For lentiviral expression of PEmax with EGFP

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

PEmax-P2A-EGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE30-His-AsnRS

Plasmid

#124105

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Asparaginyl-tRNA ligase (asnS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE60-EFG-His

Plasmid

#124126

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Elongation factor G (fusA Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE30-His-IF2

Plasmid

#124124

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Initiation factor 2 (infB Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-Neq2X7

Plasmid

#182366

Purpose

Expresses Neq2X7 DNA polymerase

Depositor

Morten Norholm

Article

Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

Insert

Neq2X7 DNA polymerase

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 15,575 results for: nol