Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

171,784 results

Showing: 5701 - 5750 of 171784 results

  3. pIR-CMV-SCN1A-Variant-1-IRES-mScarlet

    Plasmid
    #162278
    Purpose
    Eukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
    Depositor
    Al George
    Article
    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do
    Insert
    SCN1A Variant 1, stabilized (SCN1A Human)
    Tags
    IRES-mScarlet
    Expression
    Mammalian
    Mutation
    IVS intron inserted after bp2946. Silent codon c…
    Promoter
    CMV
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    162278-plasmid-map-sequence-id-338003

    Enlarge

  7. XLone-PE2

    Plasmid
    #136463
    Purpose
    Expresses prime editing 2 (PE2) enzyme upon doxycycline treatment
    Depositor
    Xiaojun Lian
    Insert
    PE2
    Expression
    Mammalian
    Promoter
    TRE3GS
    Available Since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    136463-plasmid-map-sequence-id-272540

    Enlarge

  8. pAAV-CAG-LiLac

    Plasmid
    #184570
    Purpose
    Fluorescent reporter for lactate (with lifetime and intensity response); expresses in mammalian cells and can be packaged as AAV
    Depositor
    Gary Yellen
    Article
    Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:
    Has Service
    AAV8
    Insert
    LiLac fluorescent sensor of lactate
    Use
    AAV
    Tags
    His7
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    184570-plasmid-map-sequence-id-490399

    Enlarge

  12. Shank3-HA/pRK5

    Plasmid
    #197335
    Purpose
    Plasmid expressing an antigen targeting Shank3
    Depositor
    Morgan Sheng
    Insert
    Shank3 (Shank3 Rat)
    Tags
    HA
    Expression
    Mammalian
    Available Since
    June 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    197335-plasmid-map-sequence-id-388451

    Enlarge

  16. pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4

    Plasmid
    #217971
    Purpose
    Provides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.
    Depositor
    Robert Piper
    Article
    Minard et al Biol Open. 2025 Oct 15;14(10):bio061793.
    Insert
    2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4
    Expression
    Mammalian
    Available Since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    217971-plasmid-map-sequence-id-436106

    Enlarge

  18. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

    Plasmid
    #185911
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8e-nSpG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185911-plasmid-map-sequence-id-367364

    Enlarge

  21. pGL4 [luc2P/Myc/Hygro] Vector

    Plasmid
    #236815
    Purpose
    Express Myc response element in luciferase reporter assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    Myc (MYC Human)
    Use
    Luciferase
    Mutation
    None
    Promoter
    minP
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236815-plasmid-map-sequence-id-471821

    Enlarge

  25. mCherry-7aa-cb5-pEGFP-C1

    Plasmid
    #177407
    Purpose
    To express mCherry fused to endoplasmic reticulum (ER) targeting sequence "Cb5". mCherry faces the cytosol.
    Depositor
    David Andrews
    Insert
    Cb5 tail anchor (Cyb5r4 Rat)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177407-plasmid-map-sequence-id-348154

    Enlarge

  26. LSB-hsa-miR-192-3p

    Plasmid
    #103303
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-192-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-192-3p target (MIR192 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103303-plasmid-map-sequence-id-195655

    Enlarge

  27. LSB-hsa-miR-192-5p

    Plasmid
    #103304
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-192-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-192-5p target (MIR192 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103304-plasmid-map-sequence-id-195657

    Enlarge

  42. pTipi2.1_Anti-Rho IPI-[1D4] Mouse/Rabbit HC

    Plasmid
    #221365
    Purpose
    Mammalian expression of Anti-Rho IPI-[1D4] Mouse/Rabbit HC; to be used with Anti-Rho IPI-[1D4] light chain (Plasmid 221366) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Article
    Yang et al bioRxiv, March 20, 2026
    Insert
    Anti-Rho IPI-[1D4] Mouse/Rabbit HC
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221365-plasmid-map-sequence-id-436834

    Enlarge

  43. pAAV-hSyn-hM3D(Gq)-mCherry

    Plasmid
    #50474
    Purpose
    Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM3D(Gq)-mCherry (CHRM3 Human)
    Use
    AAV
    Tags
    mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50474-plasmid-map-sequence-id-471606

    Enlarge

  44. JD150

    Plasmid
    #229485
    Purpose
    Adenoviral helper plasmid containing E2A, VA RNA I, E4orf6 and L4-22k genes
    Depositor
    Nerea Zabaleta
    Article
    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3
    Insert
    E2A, VA RNA I, E4orf6, L4-22k/33k
    Use
    AAV and Adenoviral
    Expression
    Mammalian
    Promoter
    CMV (E2A, E4orf6), Ef1a (L4-22k) and wt promoter …
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    229485-plasmid-map-sequence-id-455002

    Enlarge

  48. pLL3.7

    Plasmid
    #11795
    Purpose
    3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.
    Depositor
    Luk Parijs
    Article
    Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Promoter
    mouse U6
    Available Since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only
    11795-plasmid-map-sequence-id-237575

    Enlarge

  49. pIR-CMV-SCN2A-Variant-1-IRES-mScarlet

    Plasmid
    #162279
    Purpose
    Eukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
    Depositor
    Al George
    Article
    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do
    Insert
    SCN2A Variant 1, stabilized (SCN2A Human)
    Tags
    IRES-mScarlet
    Expression
    Mammalian
    Mutation
    IVS intron inserted after bp4551.
    Promoter
    CMV
    Available Since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162279-plasmid-map-sequence-id-321873

    Enlarge

Showing: 5701 - 5750 of 171784 results