19,627 results

1. pDestTol2CG2-U6:gRNA

   Plasmid

   #63156

   Purpose

   Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Type

   Empty backbone

   Use

   CRISPR; Tol2 destination vector for gateway

   Available Since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRK892

   Plasmid

   #71803

   Purpose

   Expression of mutated L. gasseri gusA from strong Ppgm promoter

   Depositor

   Rodolphe Barrangou, Todd Klaenhammer

   Article

   Duong et al Microb Biotechnol. 2011 May;4(3):357-67.

   Insert

   gusA3
   

   Expression

   Bacterial

   Mutation

   D573A

   Promoter

   Ppgm

   Available Since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3xnls-mScarlet3_N1

   Plasmid

   #189775

   Purpose

   Construct for labelling cell nuclei in mammalian cells using nuclear targeted mScarlet3

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   3xnls-mScarlet3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENN.AAV.hSyn.Cre.WPRE.hGH

   Plasmid

   #105553

   Purpose

   AAV expression of Cre from hSyn promoter

   Depositor

   James M. Wilson

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   Cre
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-BbsI-PITCh

   Plasmid

   #127875

   Purpose

   For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA6 asyn E46K

   Plasmid

   #105730

   Purpose

   Mammalian expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

   Insert

   alpha-synuclein E46K (SNCA Human)
   

   Expression

   Mammalian

   Mutation

   E46K

   Promoter

   CMV

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCBL101-RUBY

   Plasmid

   #199723

   Purpose

   Expresses betalain biosynthesis genes under CaMV 35S promoter

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   35S-RUBY
   

   Expression

   Plant

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. OCT4-2A-eGFP-PGK-Puro

   Plasmid

   #31938

   Purpose

   Human OCT4 genomic targeting donor

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

   Insert

   OCT4 (POU5F1 Human)
   

   Use

   Targeting donor

   Tags

   eGFP

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. PLKO.1-Scrambled

   Plasmid

   #136035

   Purpose

   Scrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   None (Scrambled)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-Sport6-CD9-pHluorin

    Plasmid

    #130905

    Purpose

    Expression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD9-pHluorin (CD9 Human, Aequorea victoria)
    

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    phluorin inserted between Asn-50 and Asn-51 in ex…

    Promoter

    CMV

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. CAG-Blimp1

    Plasmid

    #73995

    Purpose

    Plasmid expressing mouse Blimp1

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    Blimp1 gene (Prdm1 Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRKH3-ermGFP

    Plasmid

    #27169

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
    

    Expression

    Bacterial

    Mutation

    presence of an additional 183-bp region at N term…

    Available Since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-BE3

    Plasmid

    #73021

    Purpose

    Expresses BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lck-mScarlet-I

    Plasmid

    #98821

    Purpose

    In vivo visualization of the plasma membrane (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lck
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Str-Ii_VSVG-SBP-EGFP

    Plasmid

    #65300

    Purpose

    synchronize trafficking of VSVG from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    VSV-G
    

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. -1748 human cyclin D1 promoter pGL3Basic

    Plasmid

    #32726

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase; Reporter gene

    Promoter

    -1748CCND1 promoter

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-Kl-EGFP

    Plasmid

    #45532

    Depositor

    Zipora Yablonka-Reuveni

    Article

    Phelps et al FEBS J. 2016 May;283(9):1653-68. doi: 10

    Insert

    alpha Klotho (Kl Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCSC-NGN2-IRES-GFP-T2A-Sox11

    Plasmid

    #90214

    Purpose

    To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with ISL1, LHX3, FGF2 and two small molecules, forskolin and dorsomorphin.

    Depositor

    Chun-Li Zhang

    Article

    Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

    Insert

    NGN2-IRES-GFP-T2A-Sox11 (NEUROG2, Sox11 Mouse, Human)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYLL201

    Plasmid

    #53454

    Purpose

    Expresses HA-tagged mouse DNA topoisomerase IIbeta (full length cDNA, in pCS2+ backbone)

    Depositor

    Leroy Liu, Lisa Lyu

    Article

    Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.

    Insert

    HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) (Top2b Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. GABA(A) receptor subunit a1SE

    Plasmid

    #49168

    Purpose

    pHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

    Depositor

    Tija Jacob, Stephen Moss

    Article

    Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do

    Insert

    GABA(A) receptor subunit alpha-1 (Gabra1 Mouse)
    

    Tags

    myc and pHGFP (pHIuorin)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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