163,682 results

1. pAAV_BiPVe4_Gq_dTomato

   Plasmid

   #213938

   Purpose

   AAV construct expressing HA-hM3Dq-dTomato driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_Gq_dTomato

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Insert

   HA-GqDREADD-dTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Flag mTOR wt

   Plasmid

   #26603

   Purpose

   Mammalian expression of human flag-tagged mTor

   Depositor

   Jie Chen

   Article

   Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

   Insert

   mTOR (MTOR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GFP-ERK2

   Plasmid

   #37145

   Depositor

   Rony Seger

   Article

   Rubinfeld et al J Biol Chem. 1999 Oct 22;274(43):30349-5

   Insert

   ERK2 (MAPK1 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa1-6 deleted as a result of cloning strategy

   Promoter

   CMV

   Available Since

   Nov. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

4. TFORF1335

   Plasmid

   #142786

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   POU3F2 (POU3F2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDRF1-GW ymNeongreen-T2A-mCherry

   Plasmid

   #125700

   Purpose

   Produces equimolar expression of yeast codon optimized mNeongreen and mCherry

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymNeongreen-T2A-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. human beta-catenin pcDNA3

   Plasmid

   #16828

   Purpose

   Mammalian expression of human beta-catenin with Flag tag

   Depositor

   Eric Fearon

   Article

   Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

   Insert

   beta-catenin (CTNNB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. mPiezo1-IRES-eGFP

   Plasmid

   #80925

   Purpose

   expresses mouse Piezo1 with GFP

   Depositor

   Ardem Patapoutian

   Article

   Coste et al Science. 2010 Oct 1;330(6000):55-60. doi

   Insert

   mouse Piezo1 CDS (Piezo1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEBTet-SNAP-Cep152

   Plasmid

   #136825

   Purpose

   Mammalian expression of the centrosomal protein Cep152 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Cep152 (CEP152 Human, Synthetic)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICE-HA-NLS-I-PpoI

   Plasmid

   #46963

   Purpose

   For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   NLS-I-PpoI
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TET

   Available Since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-synapsin-HaloCaMP1a-EGFP

    Plasmid

    #138327

    Purpose

    chemigenetic calcium indicator

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Has Service

    AAV1 and AAV9

    Insert

    HaloCaMP1a
    

    Use

    AAV

    Tags

    EGFP, His-tag, and Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNCS mTangerine

    Plasmid

    #91763

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Tangerine (mTangerine)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    monomeric Tangerine
    

    Use

    Tags

    no tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CSII-prEF1a-mCherry-3xNLS

    Plasmid

    #125262

    Purpose

    Fluorescent reporter for cell size

    Depositor

    Jan Skotheim

    Article

    Berenson et al Mol Biol Cell. 2019 Nov 15;30(24):2985-2

    Insert

    mCherry-3xNLS
    

    Use

    Lentiviral

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCW57-MCS1-2A-MCS2

    Plasmid

    #71782

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AiP12828 - pAAV-AiE0456m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2828)

    Plasmid

    #224006

    Purpose

    AiE0456m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    minBG

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR TERRA

    Plasmid

    #111383

    Purpose

    TERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

    Depositor

    Klaus Holzmann

    Article

    Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

    Insert

    TERRA
    

    Use

    Gateway entry cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. gag-COM-pol

    Plasmid

    #211373

    Purpose

    Packaging plasmid for v3b PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    MMLVgag-COM-pol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG1160 mCherry-FIREtag

    Plasmid

    #205164

    Purpose

    Expresses mCherry -FIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    mCherry-FIREtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MSCV-BCR-ABL1-p190-IRES-EGFP

    Plasmid

    #219964

    Purpose

    Express human BCR-ABL1-P190 in mammalian cells

    Depositor

    Mohamed Fareh

    Insert

    BCR-ABL1-P190 (ABL1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AbVec2.0-IGHG1

    Plasmid

    #80795

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-FLAG-CRBN-pGK-HYG

    Plasmid

    #107380

    Purpose

    Mammalian expression of FLAG-CRBN

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    cereblon (CRBN Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET24b-APEX2

    Plasmid

    #111702

    Purpose

    For IPTG-inducible expression of APEX2 in E. coli

    Depositor

    Jessica Seeliger

    Article

    Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

    Insert

    APEX2
    

    Use

    Tags

    6xHis and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-TrxRFP1

    Plasmid

    #98996

    Purpose

    mammalian expression of a biosensor to monitor thioredoxin redox dynamics

    Depositor

    Huiwang Ai

    Article

    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

    Insert

    human thioredoxin 1, GS-rich linker, fused with a redox-sensitive RFP
    

    Use

    Tags

    His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pWY82

    Plasmid

    #65721

    Purpose

    Plasmid with 2.5 kb of telomere sequence

    Depositor

    James Birchler

    Article

    Yu et al Proc Natl Acad Sci U S A. 2006 Nov 14;10

    Insert

    Telomere
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMU-ACTLr

    Plasmid

    #61193

    Purpose

    Fluorescent reporter for F-actin LifeAct-mCherry expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    LifeAct-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available Since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Gal:mCherry-CRY2-uTEV1Δ(220-242)

    Plasmid

    #135452

    Purpose

    Yeast expression of uTEV1Δ fused to CRY2 and mCherry

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    mCherry-CRY2-TEV1Δ(220-242)
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Gal

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. EWS-GFP

    Plasmid

    #164701

    Purpose

    Expression of EWS-GFP

    Depositor

    Cheryl Arrowsmith

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMOS008: GCaMP6F calcium sensor (cytosolic)

    Plasmid

    #163045

    Purpose

    GCaMP6F calcium sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    GCaMP6F
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. hZMPSTE24-3FLAG_pQCXIP

    Plasmid

    #89759

    Purpose

    Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.

    Depositor

    Martin Dorf

    Article

    Fu et al J Exp Med. 2017 Feb 28. pii: jem.2016127

    Insert

    Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
    

    Use

    Retroviral; Bicistronic expression: puromycin re…

    Tags

    9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…

    Expression

    Mammalian

    Mutation

    Y253H in ZMPSTE24 (see depositor comments below)

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pNCS mHoneydew

    Plasmid

    #91760

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Honeydew (mHoneydew)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    monomeric Honeydew
    

    Use

    Tags

    no tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CROP_C9_Puro_ERBB2

    Plasmid

    #183287

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets ERBB2 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    ERBB2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge