165,965 results

1. BirA in pET28a (w400-2)

   Plasmid

   #26624

   Depositor

   Eric Campeau

   Insert

   BirA
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-Hygro

   Plasmid

   #127763

   Purpose

   Used for expression of sgRNA in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. psgRNA

   Plasmid

   #114005

   Purpose

   express sgRNA. ColE1, Kan

   Depositor

   David Bikard

   Article

   Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

   Insert

   gRNA
   

   Promoter

   BBa_J23119

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. MaLionG

   Plasmid

   #113906

   Purpose

   Green ATP sensor

   Depositor

   Tetsuya Kitaguchi

   Article

   Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

   Insert

   MaLionG
   

   Expression

   Mammalian

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCX-TRI-2A

   Plasmid

   #74674

   Purpose

   mammalian expression of TRI-2A (hHO1/F2A/hCD73/F2A/hCD39)

   Depositor

   Roberto Giovannoni

   Article

   Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

   Inserts

   HO1 (HMOX1 Human)
   CD73 (NT5E Human)
   CD39 (ENTPD1 Human)
   

   Tags

   F2A

   Expression

   Mammalian

   Promoter

   pCAGGS

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ230 (LbCpf1)

   Plasmid

   #86210

   Purpose

   LbCpf1 Gateway entry plasmid

   Depositor

   Yiping Qi

   Article

   Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

   Insert

   LbCpf1
   

   Use

   CRISPR; Gateway compatible lbcpf1 entry clone

   Tags

   5' and 3' NLS

   Expression

   Plant

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAF0084 Ef1a-Cp36-2a-EGFP

   Plasmid

   #193462

   Purpose

   Expression of Cp36 recombinase from Ef1a promoter

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Cp36
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. POLG-pcDNA3.1

   Plasmid

   #191487

   Purpose

   Expresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.

   Depositor

   Hans Zempel

   Insert

   POLG (POLG Human)
   

   Tags

   V5 tag, 6X His

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-RLuc8

   Plasmid

   #87121

   Purpose

   Mammalian expression vector expressing mutant version of the reporter gene-Renilla Luciferase (Rluc8), which is more stable in mouse serum and has more light output.

   Depositor

   Sanjiv Sam Gambhir

   Article

   Loening et al Protein Eng Des Sel. 2006 Sep;19(9):391-

   Insert

   Renilla Luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   A55T, C124A, S130A, K136R, A143M, M185V, M253L, a…

   Promoter

   CMV

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA Flag Lamp2b-HA

    Plasmid

    #71292

    Purpose

    Flag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.

    Depositor

    Joshua Leonard

    Article

    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

    Insert

    Lamp2b (LAMP2 Human)
    

    Tags

    Flag, HA, and Lamp2 signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCV-hCD19

    Plasmid

    #127889

    Purpose

    Expresses hCD19

    Depositor

    Anjana Rao

    Article

    Chen et al Nature. 2019 Mar;567(7749):530-534. doi:

    Insert

    hCD19 (CD19 Human)
    

    Use

    Retroviral

    Tags

    no tags

    Mutation

    none

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. mEmerald-MAP4-N-10

    Plasmid

    #54153

    Purpose

    Localization: Microtubules, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    MAP4 (MAP4 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pVS1-VIR2

    Plasmid

    #134745

    Purpose

    Virulence and replication helper

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    vir gene cluster
    

    Use

    Vir gene expression

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28-hXRCC1

    Plasmid

    #70759

    Purpose

    bacterial expression of human XRCC1

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    XRCC1 (XRCC1 Human)
    

    Tags

    6-HIS

    Expression

    Bacterial

    Promoter

    T7/lac operon

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWZL-Blast FAPwt

    Plasmid

    #210643

    Purpose

    Expresses the FAP (Fibroblast Activation Protein) protein.

    Depositor

    Jing Yang

    Article

    Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

    Insert

    Fibroblast Activation Protein Alpha (FAP Human)
    

    Use

    Retroviral

    Mutation

    none

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQE-60NA-proinsulin-msGFP2

    Plasmid

    #160466

    Purpose

    Encodes a fusion of human proinsulin and the monomeric superfolder GFP2

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    proinsulin-msGFP2
    

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Orai1 YFP

    Plasmid

    #19756

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    Orai1 (ORAI1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Oct. 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-3XpolyA-3XPC-miniCMV-mCherry-WPRE-insulator

    Plasmid

    #168291

    Purpose

    optimized miniCMV (OminiCMV)

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    mCherry
    

    Expression

    Mammalian

    Promoter

    miniCMV

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. v3em-Cterm-PE2max-∆RNaseH-dualU6

    Plasmid

    #198735

    Purpose

    AAV genome encoding C-terminal PE2max and U6 expression cassettes

    Depositor

    David Liu

    Article

    Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

    Insert

    NpuC-CtermPE2max∆RNaseH
    

    Use

    AAV

    Promoter

    Cbh

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. SepRS(2)/pSertRNA(B4)/EF-Sep

    Plasmid

    #173897

    Purpose

    The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.

    Depositor

    Jason W Chin

    Article

    Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d

    Inserts

    SepRS(2)
    pSertRNA
    EF-Sep
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. Fuw-dCas9-Dnmt3a

    Plasmid

    #84476

    Purpose

    Lentiviral construct to express dCas9 fused with Dnmt3a

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Dnmt3a (DNMT3A Synthetic, Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pSpdCas9-huTET1CD-T2A-GFP (PX458)

    Plasmid

    #129025

    Purpose

    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA cloning site
    

    Tags

    HA-Tag, NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pGP-CMV-GCaMP6f

    Plasmid

    #40755

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Insert

    GCaMP6f
    

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLKO.1 Sox2 HM a

    Plasmid

    #26353

    Depositor

    Matthew Meyerson

    Article

    Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

    Insert

    Sox2 shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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25. pUltra-Plus

    Plasmid

    #161775

    Purpose

    3rd generation lentiviral multicistronic vector for mammalian expression of three genes of interest

    Depositor

    Jorge Luis Arias-Arias

    Type

    Empty backbone

    Use

    Lentiviral; Multicistronic

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMRE131

    Plasmid

    #118485

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mTurquoise2
    

    Expression

    Bacterial

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCMV-T7 RNA POL

    Plasmid

    #138352

    Purpose

    Expressing T7 RNA Polymerase in mammalian cells

    Depositor

    Baisong Lu

    Insert

    T7 RNA POL
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 4xNLS-pMJ915v2-sfGFP

    Plasmid

    #88921

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 476 p5E-mcs

    Plasmid

    #26029

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Type

    Empty backbone

    Use

    Entry vector

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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30. pAAV-CKIIa-stGtACR1-FusionRed

    Plasmid

    #105679

    Purpose

    Expresses a soma-targeted GtACR1 in mammalian neurons under the CaMKIIa promoter

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

    Insert

    stGtACR1
    

    Use

    AAV

    Tags

    FusionRed and Kv2.1

    Expression

    Mammalian

    Promoter

    CaMKIIa

    Available Since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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