We narrowed to 11,549 results for: NSI

1. pDC146

   Plasmid

   #169366

   Purpose

   Expresses Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96

   Depositor

   Axel Brunger

   Article

   Cipriano et al J Biol Chem. 2013 Aug 9;288(32):23436-45

   Insert

   Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96
   

   Use

   Tags

   6 his tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHR-MCS-YFP-NLS

   Plasmid

   #145274

   Purpose

   Mammalian Expression of Nuclear YFP with N-terminal MCS and SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   MCS and YFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Split-RESCUE-C

   Plasmid

   #170154

   Purpose

   AAV vector carrying the RESCUE-DDC-λN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   RESCUE-DDC-λN-NES
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. G273D HA-dynamin 1 pUHD10-3

   Plasmid

   #34689

   Depositor

   Sandra Schmid

   Article

   Damke et al J Cell Biol. 1995 Oct;131(1):69-80.

   Insert

   dynamin 1 (DNM1 Human)
   

   Use

   Tags

   HA (variant)

   Expression

   Mammalian

   Mutation

   G273D

   Promoter

   Available Since

   Jan. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

5. SERA5-bio-His

   Plasmid

   #50817

   Purpose

   Expresses enzymatically monobiotinylated full length SERA5

   Depositor

   Gavin Wright

   Article

   Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

   Insert

   SERA5
   

   Use

   Tags

   ratCD4d3+4,biotinylation sequence, 6xHis

   Expression

   Mammalian

   Mutation

   all potential N-linked glycosylation sites (N-X-S…

   Promoter

   CMV

   Available Since

   Feb. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEM-sgIGR_dual-MTS-3x-Set I

   Plasmid

   #162764

   Purpose

   Separately expressing 3 unique sgIGR_dual-MTS (SetI)

   Depositor

   Antony K Chen

   Article

   Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

   Insert

   sgIGR_dual-MTS_1; sgIGR_dual-MTS_2; sgIGR_dual-MTS_3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMXs-1002

   Plasmid

   #31711

   Depositor

   Duanqing Pei

   Article

   Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

   Insert

   miR-302b, c, a, d
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pLentiGuide_mCherry_sgEPAS1#2

   Plasmid

   #183123

   Purpose

   pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   sgEPAS1-2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCA-ATG-intron-tdT3Myc

   Plasmid

   #36883

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Insert

   tdTomato-3Myc
   

   Use

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   beta-globin intron containing a loxP site inserte…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer)

   Available Since

   Aug. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EK0499 SFFV-mCherry-SGc(PPIB.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191183

    Purpose

    Expression of PPIB sensor in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:PPIB.s1:EGFP-3xMS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAGGS EFF-1::V5

    Plasmid

    #133584

    Purpose

    Expresses EFF-1 A full length protein in mammalian cells

    Depositor

    Benjamin Podbilewicz

    Article

    Avinoam et al Science. 2011 Apr 29;332(6029):589-92. d

    Insert

    EFF-1::V5
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EK0289 SFFV-mCherry-SG(s17d9b)SG-EGFP (FLP-IN)

    Plasmid

    #191142

    Purpose

    Expression of Sensor 2 (for EK0211) in mammalian cells; mCherry marker, EGFP output.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s17d9b:EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-GATA2-DNzf

    Plasmid

    #1291

    Depositor

    Gokhan Hotamisligil

    Article

    Tong et al Science 2000 Oct 6;290(5489):134-8.

    Insert

    GATA2 (GATA2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S263G and M434V

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pRK5_tAKARγ

    Plasmid

    #119915

    Purpose

    Mammalian expression of the tAKARγ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARγ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAID1.2-CMV-NmScarlet-mAID

    Plasmid

    #140618

    Purpose

    Expression of OsTIR1 and mScarlet-mAID protein in DT40 cells under the control of CMV promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBL119-2

    Plasmid

    #104336

    Purpose

    Expresses thsR from a TetR regulated promoter under ATC control. Expresses sfGFP from a thsR regulated promoter. Expresses mCherry from a constituative promoter.

    Depositor

    Jeffrey Tabor

    Article

    Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

    Insert

    Thiosulfate response regulator
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLTetO1

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MSRP5-bio-His

    Plasmid

    #50805

    Purpose

    Expresses enzymatically monobiotinylated full length MSRP5

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    MSRP5
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP Snail 4SA

    Plasmid

    #16227

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    4SA: S108A, S112A, S116A, S120A

    Promoter

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

19. IFI16-(192-393)-HinA

    Plasmid

    #35052

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16-(192-393)-HinA (IFI16 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains HinA domain: aa 192-393

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA-GATA2-DC+Nzf

    Plasmid

    #1293

    Depositor

    Gokhan Hotamisligil

    Article

    Tong et al Science 2000 Oct 6;290(5489):134-8.

    Insert

    GATA2 (GATA2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

21. GFP Snail 2A4E

    Plasmid

    #16231

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    2A4E: S97A, S101A, S108E, S112E, S116E, S120E

    Promoter

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

22. H2B-mScarlet- hMeikin(1-332, E151R, R154E)-mNeonGreen

    Plasmid

    #174715

    Purpose

    Stable expression of fluorescent Separase cleavage sensor with human Meikin with Separase-resistant mutations

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Meikin
    

    Use

    Retroviral

    Tags

    H2B-mScarlet and mNeonGreen

    Expression

    Mammalian

    Mutation

    1-332, E151R, R154E

    Promoter

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pZH511

    Plasmid

    #102665

    Purpose

    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMM2_12

    Plasmid

    #183061

    Purpose

    Control for biosensor in pMM2_9: BM3R1-HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus

    Depositor

    Yvonne Nygård

    Article

    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

    Inserts

    mCherry
    BM3R1-HAA1-mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    mCherry and mTurquoise2

    Expression

    Yeast

    Mutation

    Promoter

    HAA1 and Synthetic promoter

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMM2_10

    Plasmid

    #183059

    Purpose

    Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus

    Depositor

    Yvonne Nygård

    Article

    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

    Inserts

    mCherry
    HAA1-mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    mCherry and mTurquoise2

    Expression

    Yeast

    Mutation

    Promoter

    HAA1 and Synthetic promoter

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMM2_9

    Plasmid

    #183058

    Purpose

    Biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under HAA1p and mCherry under BM3R1ENO1cp with flanks for genome integration in toHO locus

    Depositor

    Yvonne Nygård

    Article

    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

    Inserts

    mCherry
    BM3R1-HAA1-mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    mCherry and mTurquoise2

    Expression

    Yeast

    Mutation

    Promoter

    HAA1 and Synthetic promoter

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMM2_11

    Plasmid

    #183060

    Purpose

    Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsENO1cp with flanks for genome integration into HO locus

    Depositor

    Yvonne Nygård

    Article

    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

    Inserts

    mCherry
    HAA1-mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    mCherry and mTurquoise2

    Expression

    Yeast

    Mutation

    Promoter

    HAA1 and Synthetic promoter

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pWPI-NP1

    Plasmid

    #65060

    Purpose

    Constitutive expression of NP1

    Depositor

    Ramon Trullas

    Article

    Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

    Insert

    Neuronal Pentraxin 1 (Nptx1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    * see comments

    Promoter

    EF1alpha

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pOPTXLUC

    Plasmid

    #68504

    Purpose

    cyclic nucleotide reporter for bacteria or plant cells

    Depositor

    Helen Irving

    Article

    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

    Insert

    OPTX promoter (AT1G33440 Mustard Weed)
    

    Use

    Tags

    luciferase

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    OPTX

    Available Since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Flag Snail 4SE

    Plasmid

    #16223

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    4SE: S108E, S112E, S116E, S120E

    Promoter

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

31. PFD1130w-bio-His

    Plasmid

    #50823

    Purpose

    Expresses enzymatically monobiotinylated full length PFD1130w

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    PFD1130w
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDGB3_omega1

    Plasmid

    #68238

    Purpose

    GoldenBraid level 1 Omega plant expression vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDGB3_alpha2

    Plasmid

    #68229

    Purpose

    GoldenBraid level 1 Alpha plant expression vector.

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>

    Plasmid

    #72240

    Purpose

    Histidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    GLT1 GAL1p 24xPP7 V3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSW179 - pML1

    Plasmid

    #115977

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Jan Lohmann

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pML1
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-Flag-YAP-del 50-59

    Plasmid

    #33065

    Depositor

    Kunliang Guan

    Article

    Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 50-59

    Promoter

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

37. pMC8b-XI3up

    Plasmid

    #176170

    Purpose

    yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-3

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    5' homology for integration site XI-3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMC7-XI3dw

    Plasmid

    #176171

    Purpose

    yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-3

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    3' homology for integration site XI-3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMC8b-XI5up

    Plasmid

    #176172

    Purpose

    yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-5

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    5' homology for integration site XI-5
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMC7-XI5dw

    Plasmid

    #176173

    Purpose

    yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-5

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    3' homology for integration site XI-5
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge