We narrowed to 12,031 results for: cel.2

1. pAA1

   Plasmid

   #154161

   Purpose

   Firefly luciferase under the control of 3 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   12 wt NF-κB sites were replaced with Rel1A-specif…

   Promoter

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKM62

   Plasmid

   #154160

   Purpose

   Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel2-specific (VII) NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   4 wt NF-κB sites were replaced with Rel2-specific…

   Promoter

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEThT-MAHS delta MTP

   Plasmid

   #90032

   Purpose

   To express His-tagged MAHS in bacteria

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

   Insert

   MAHS
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Deletion of mitochondria targeting peptide

   Promoter

   T7

   Available Since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mCherry-miniSOG-Zyxin-6

   Plasmid

   #55101

   Purpose

   Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   Zyxin (ZYX Human)
   

   Use

   Tags

   mCherry-miniSOG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pPP441

   Plasmid

   #158801

   Purpose

   Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   CasPhi-2
   

   Use

   Tags

   2xSV40NLS-2xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-CalfluxCTN

   Plasmid

   #190068

   Purpose

   Expresses BRET Ca2+ sensor CalfluxCTN in mammalian cells

   Depositor

   Carl Johnson

   Article

   Cumberbatch et al Sci Signal. 2022 Aug 16;15(747):eabq7618

   Insert

   CalfluxCTN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDisplay-ZIBG2

   Plasmid

   #169458

   Purpose

   cell surface localized zinc sensor with ~ 300 nM Kd

   Depositor

   Huiwang Ai

   Article

   Chen et al Anal Chem. 2019 Oct 1;91(19):12212-12219

   Insert

   ZIBG2
   

   Use

   Tags

   pDisplay backbone elements for export and membran…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mg180

   Plasmid

   #34660

   Depositor

   Miriam Goodman

   Article

   Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

   Insert

   slo-1(A2;B0;C1) (slo-1 Nematode)
   

   Use

   Xenopus oocyte expression

   Tags

   Xenopus beta-globulin 3' UTR and Xenopus bet…

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKDC.015

   Plasmid

   #184992

   Purpose

   Express -Eco2 RT and ncRNA in human cells

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco2: RT and ncRNA(wt), a1/a2 length: 13
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Human codon optimized RT

   Promoter

   TetOn-3g

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKS2311

    Plasmid

    #37835

    Depositor

    Gary Silverman

    Article

    Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

    Insert

    Pnhx-2cpl-1W32A;Y35A::YFP (cpl-1 Nematode)
    

    Use

    Tags

    YFP

    Expression

    Worm

    Mutation

    W32A;Y35A

    Promoter

    nhx-2

    Available Since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CMV-Y-GECO2m

    Plasmid

    #105070

    Purpose

    A yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

    Insert

    Y-GECO2m
    

    Use

    Tags

    6x His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTorPE-Y-GECO2.2s

    Plasmid

    #105069

    Purpose

    A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

    Depositor

    Robert Campbell

    Article

    Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

    Insert

    Y-GECO2.2s
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. H5_fold-0 _Chantal

    Plasmid

    #201826

    Purpose

    Expresses H5_fold-0_Chantal in bacterial cells

    Depositor

    Nobuyasu Koga

    Article

    Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

    Insert

    H5_fold-0 _Chantal
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTorPE-Y-GECO2m

    Plasmid

    #105066

    Purpose

    A yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in bacterial cells

    Depositor

    Robert Campbell

    Article

    Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

    Insert

    Y-GECO2m
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTorPE-Y-GECO2s

    Plasmid

    #105067

    Purpose

    A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

    Depositor

    Robert Campbell

    Article

    Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

    Insert

    Y-GECO2s
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTorPE-Y-GECO2.1s

    Plasmid

    #105068

    Purpose

    A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

    Depositor

    Robert Campbell

    Article

    Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

    Insert

    Y-GECO2.1s
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mCherry-FKBP-TPD54(L53P,L67P)

    Plasmid

    #172458

    Purpose

    Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag L53P,L67P mutant

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    TPD54 (TPD52L2 Human)
    

    Use

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Mutation

    changed Leucines 53 and 67 to proline (breaks coi…

    Promoter

    CMV

    Available Since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-hSTING (N154S)

    Plasmid

    #214160

    Purpose

    Transient expression of gain-of-function STING mutant

    Depositor

    Nan Yan

    Article

    Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

    Insert

    STING (N154S) (STING1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N154S

    Promoter

    CMV

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSFDuet-1_mNeongreen-Erythropoietin

    Plasmid

    #182422

    Purpose

    Bacterial expression of mNeongreen-Erythropoietin fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    mNeongreen-eythropoietin (EPO Human)
    

    Use

    Tags

    mNeongreen

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSFDuet-1_VNp-DNAseI

    Plasmid

    #182409

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-DNaseI fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-DNase I (DNASE1 Bovine)
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MAC_N_AURKB

    Plasmid

    #111682

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    AURKB (AURKB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. dmBACCS2-IRES-dOrai-IRES-mCherry

    Plasmid

    #72894

    Purpose

    Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2-IRES-dOrai-IRES-mCherry
    

    Use

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-hSTING (V155M)

    Plasmid

    #214161

    Purpose

    Transient expression of gain-of-function STING mutant

    Depositor

    Nan Yan

    Article

    Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

    Insert

    STING (V155M) (STING1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V155M

    Promoter

    CMV

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSH-EFIRES-P-AtAFB2-mCherry

    Plasmid

    #129716

    Purpose

    AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    EF1a

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1-HA-mSTING (V154M)

    Plasmid

    #214159

    Purpose

    Transient expression of gain-of-function STING mutant

    Depositor

    Nan Yan

    Article

    Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

    Insert

    STING (V154M) (Sting1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N153S

    Promoter

    CMV

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEMS2232

    Plasmid

    #158401

    Purpose

    High Copy number plasmid containing Ple316 (NR2E1 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple316 (NR2E1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Ple316

    Available Since

    Feb. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pcDNA3.1-HA-mSTING (N153S)

    Plasmid

    #214158

    Purpose

    Transient expression of gain-of-function STING mutant

    Depositor

    Nan Yan

    Article

    Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

    Insert

    STING (N153S) (Sting1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N153S

    Promoter

    CMV

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDNA3-HA-Akt1-K179M

    Plasmid

    #73409

    Purpose

    Expresses kinase-inactive mutant of Akt with HA tag

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    changed K179M to make Kinase inactive mutant

    Promoter

    CMV

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. OPA1-isoform2

    Plasmid

    #70174

    Purpose

    Mammalian expression of hsOPA1 isoform 2

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    hsOPA1 isoform 2 (OPA1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA-IgKappa-myc-dL5- 2XG4S-GPI

    Plasmid

    #153308

    Purpose

    Expresses GPI-anchored dL5** in mammalian cells. (FAP-GPI)

    Depositor

    Marcel Bruchez

    Article

    Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

    Insert

    IgKappa-myc-dL5-2XG4S-GPI
    

    Use

    Tags

    myc-dL5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. StrKDEL-IRES-ManII-mScarlet-i

    Plasmid

    #117274

    Purpose

    Expresses an Str-KDEL Hook for the RUSH system and separately mannosidase II-mScarlet-i

    Depositor

    David Stephens

    Article

    McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180

    Inserts

    partial sequence of mannosidase II (Man2a1 Mouse)
    Streptavidin-KDEL
    

    Use

    Tags

    mScarlet-i

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV and IRES

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PH-Btk(R28C)-GFP

    Plasmid

    #51464

    Purpose

    Non-PIP3 binding control for PH-Btk-GFP

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    BTK (aa 1–177) (BTK Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    R28C

    Promoter

    CMV

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pAAV-CAGGS-Flex/3'USS-GFP(ATG mut)

    Plasmid

    #197885

    Purpose

    Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGM190

    Plasmid

    #69614

    Purpose

    inducible gene expression in Streptomyces

    Depositor

    Günther Muth

    Article

    Reuther et al Mol Microbiol. 2006 Jul;61(2):436-46. do

    Inserts

    aphII
    tsr
    rep
    sso
    TipA promoter
    dso
    

    Use

    Streptomyces- e. coli shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. hCEP120-Myc

    Plasmid

    #50381

    Purpose

    Tet-On inducible expression of Myc-tagged hCEP120

    Depositor

    Tang Tang

    Article

    Lin et al J Cell Biol. 2013 Jul 22;202(2):211-9. d

    Insert

    Human CEP120 (CEP120 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    This hCEP120 contains 4 SNPs (rs6876883, rs659544…

    Promoter

    CMV-2xTetO

    Available Since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pENN.AAV.CB7.CI.CNDP2-flag.WPRE.rBG

    Plasmid

    #132685

    Purpose

    AAV plasmid encoding mouse CNDP2 with C-terminal flag tag

    Depositor

    Jonathan Long

    Article

    Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

    Insert

    Carnosine dipeptidase 2 (Cndp2 Mouse)
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Chicken b-actin

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCDNA3-HA-Akt1-aa1-149

    Plasmid

    #73410

    Purpose

    Expresses HA tagged aa1-149 of AKT1

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Expresses aa 1-149 only of AKT1

    Promoter

    CMV

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

38. pCDNA3-HA-Akt1-aa120-433

    Plasmid

    #73411

    Purpose

    Expresses HA tagged aa120-433 AKT1

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Expresses aa 120-433 of AKT1

    Promoter

    CMV

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

39. pAAV-EF1α-Flex/3'USS-mCherry(ATG full mut)

    Plasmid

    #197893

    Purpose

    Can be used to generate AAV virus that will express mCherry in the presence of Cre and the leakage expression is significantly reduced without Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    EF1a

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV-hNR2F2-RFP

    Plasmid

    #22926

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    human nuclear receptor subfamily 2 group F member 2 promoter driving DsRedExpress (NR2F2 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBabe HA-∆Raf1(S642A):ER* puro

    Plasmid

    #72572

    Purpose

    Expresses hormone-responsive, feedback-resistant Raf allele with an N-terminal HA tag.

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

    Insert

    Raf1 (RAF1 Human)
    

    Use

    Retroviral

    Tags

    ER* and HA

    Expression

    Mammalian

    Mutation

    amino acids 306-648, serine 642 mutated to alanine

    Promoter

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

42. dmBACCS2-IRES-dOrai-IRES-GFP

    Plasmid

    #72893

    Purpose

    Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2-IRES-dOrai-IRES-GFP
    

    Use

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. dmBACCS2NS-IRES-dOrai-IRES-GFP

    Plasmid

    #72895

    Purpose

    Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2NS-IRES-dOrai-IRES-GFP
    

    Use

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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44. pCDNA3-HA-Akt1-aa1-408

    Plasmid

    #73412

    Purpose

    Expresses HA tagged aa1-408 of AKT1

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Expresses aa 1-408 of AKT1

    Promoter

    CMV

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

45. CIB-VP16 (B1014)

    Plasmid

    #92036

    Purpose

    Expresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CIB1 (CIB1 Mustard Weed)
    

    Use

    Tags

    Fusion of plant CIB1 (intact NLS) with VP16 activ…

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pGL3Basic-miR126-EGFL7-Promoter

    Plasmid

    #32244

    Depositor

    Charles Lowenstein

    Article

    Harris et al Arterioscler Thromb Vasc Biol. 2010 Oct;

    Insert

    miR-126 Promoter 5' UTR (MIR126, EGFL7 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter region 5' UTR

    Promoter

    5' UTR

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pYL214

    Plasmid

    #171031

    Purpose

    CRISPR plasmids encodes Cas9 and a sgRNA targeting EZH2 exon 2 - intron 2 junction (sequence: GCAGACGAGCTGATGAAGTAA)

    Depositor

    Thomas Cech

    Article

    Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

    Insert

    EZH2 (EZH2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pRSFDuet-1_VNp-FGF21

    Plasmid

    #182407

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-fgf21 fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-fgf21 (FGF21 Human)
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pAAV-CAGGS-Flex/3'USS(Luc)-GFP

    Plasmid

    #197887

    Purpose

    Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. HA-VHL C162F-pRc/CMV

    Plasmid

    #22042

    Depositor

    William Kaelin

    Article

    Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    point mutant, converting aa 162 from Cysteine to …

    Promoter

    Available Since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge